278: Gene Expression Profiling of Prostatic Epithelial Cells in Two-Dimensional Versus Three-Dimensional Cultures

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Toshiyuki Tsunoda ◽  
Junichi Inocuchi ◽  
Darren Tyson ◽  
Seiji Naito ◽  
David K. Ornstein
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Three Dimensional ◽  
Two Dimensional

scholarly journals Assessing Various Control Samples for Microarray Gene Expression Profiling of Laryngeal Squamous Cell Carcinoma

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 588
Author(s):  
Adam Ustaszewski ◽  
Magdalena Kostrzewska-Poczekaj ◽  
Joanna Janiszewska ◽  
Malgorzata Jarmuz-Szymczak ◽  
Malgorzata Wierzbicka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Epithelial Cells ◽  
Cell Carcinoma ◽  
Gene Expression Profiling ◽  
Cell Lines ◽  
Squamous Cell ◽  
Expression Profiling ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Control Samples

Selection of optimal control samples is crucial in expression profiling tumor samples. To address this issue, we performed microarray expression profiling of control samples routinely used in head and neck squamous cell carcinoma studies: human bronchial and tracheal epithelial cells, squamous cells obtained by laser uvulopalatoplasty and tumor surgical margins. We compared the results using multidimensional scaling and hierarchical clustering versus tumor samples and laryngeal squamous cell carcinoma cell lines. A general observation from our study is that the analyzed cohorts separated according to two dominant factors: “malignancy”, which separated controls from malignant samples and “cell culture-microenvironment” which reflected the differences between cultured and non-cultured samples. In conclusion, we advocate the use of cultured epithelial cells as controls for gene expression profiling of cancer cell lines. In contrast, comparisons of gene expression profiles of cancer cell lines versus surgical margin controls should be treated with caution, whereas fresh frozen surgical margins seem to be appropriate for gene expression profiling of tumor samples.

Gene expression profiling of human bronchial epithelial cells exposed to seasonal Milan particulate matter

2014 ◽  
Vol 229 ◽  
pp. S71
Author(s):  
Eleonora Longhin ◽  
Laura Capasso ◽  
Cristina Battaglia ◽  
Cristina Cosentino ◽  
Maria Carla Proverbio ◽  
...  
Keyword(s):  
Gene Expression ◽  
Particulate Matter ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

scholarly journals Noninvasive method for obtaining RNA from buccal mucosa epithelial cells for gene expression profiling

BioTechniques ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 484-487 ◽  
Author(s):  
Avrum Spira ◽  
Jennifer Beane ◽  
Frank Schembri ◽  
Gang Liu ◽  
Chunming Ding ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Buccal Mucosa ◽  
Expression Profiling ◽  
Noninvasive Method

scholarly journals Cellular-resolution gene expression profiling in the neonatal marmoset brain reveals dynamic species- and region-specific differences

2021 ◽  
Vol 118 (18) ◽  
pp. e2020125118
Author(s):  
Yoshiaki Kita ◽  
Hirozumi Nishibe ◽  
Yan Wang ◽  
Tsutomu Hashikawa ◽  
Satomi S. Kikuchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Neural Circuit ◽  
Expression Patterns ◽  
Three Dimensional ◽  
Control Of Gene Expression ◽  
Cellular Resolution

Precise spatiotemporal control of gene expression in the developing brain is critical for neural circuit formation, and comprehensive expression mapping in the developing primate brain is crucial to understand brain function in health and disease. Here, we developed an unbiased, automated, large-scale, cellular-resolution in situ hybridization (ISH)–based gene expression profiling system (GePS) and companion analysis to reveal gene expression patterns in the neonatal New World marmoset cortex, thalamus, and striatum that are distinct from those in mice. Gene-ontology analysis of marmoset-specific genes revealed associations with catalytic activity in the visual cortex and neuropsychiatric disorders in the thalamus. Cortically expressed genes with clear area boundaries were used in a three-dimensional cortical surface mapping algorithm to delineate higher-order cortical areas not evident in two-dimensional ISH data. GePS provides a powerful platform to elucidate the molecular mechanisms underlying primate neurobiology and developmental psychiatric and neurological disorders.

scholarly journals Global Gene Expression Profiling of Human Lung Epithelial Cells After Exposure to Nanosilver

2012 ◽  
Vol 130 (1) ◽  
pp. 145-157 ◽  
Author(s):  
Rasmus Foldbjerg ◽  
Eveline S. Irving ◽  
Yuya Hayashi ◽  
Duncan S. Sutherland ◽  
Kasper Thorsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Global Gene Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Global Gene Expression Profiling ◽  
Human Lung Epithelial Cells

scholarly journals Gene Expression Profiling of Gliadin Effects on Intestinal Epithelial Cells Suggests Novel Non-Enzymatic Functions of Pepsin and Trypsin

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e66307 ◽  
Author(s):  
Amarjit Parmar ◽  
Dario Greco ◽  
Jarkko Venäläinen ◽  
Massimiliano Gentile ◽  
Emma Dukes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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