Aspergillus oryzae NRRL 35191 from coffee, a non-toxigenic endophyte with the ability to synthesize kojic acid

2011 ◽  
Vol 11 (1) ◽  
pp. 263-267 ◽  
Author(s):  
Fabio C. Chaves ◽  
Thomas J. Gianfagna ◽  
Madhu Aneja ◽  
Francisco Posada ◽  
Stephen W. Peterson ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Kojic Acid

Overexpression of a novel gene Aokap2 affects the growth and kojic acid production in Aspergillus oryzae

2022 ◽  
Author(s):  
Yuzhen Li ◽  
Huanxin Zhang ◽  
Ziming Chen ◽  
Junxia Fan ◽  
Tianming Chen ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Acid Production ◽  
Kojic Acid ◽  
Novel Gene ◽  
Kojic Acid Production

Improvement of production of Kojic acid by a mutant strain Aspergillus oryzae, MK107-39

2001 ◽  
Vol 91 (3) ◽  
pp. 272-276 ◽  
Author(s):  
Takafumi Futamura ◽  
Mitsuyasu Okabe ◽  
Takayoshi Tamura ◽  
Kyouko Toda ◽  
Toshio Matsunobu ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Aspergillus Oryzae ◽  
Kojic Acid

Improvement of kojic acid production in Aspergillus oryzae B008 mutant strain and its uses in fermentation of concentrated corn stalk hydrolysate

2013 ◽  
Vol 37 (6) ◽  
pp. 1095-1103 ◽  
Author(s):  
Shoubao Yan ◽  
Huijuan Tang ◽  
Shunchang Wang ◽  
Liangting Xu ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Aspergillus Oryzae ◽  
Acid Production ◽  
Kojic Acid ◽  
Corn Stalk ◽  
Kojic Acid Production

scholarly journals Aspergillus oryzae laeARegulates Kojic Acid Synthesis Genes

2011 ◽  
Vol 75 (9) ◽  
pp. 1832-1834 ◽  
Author(s):  
Ken ODA ◽  
Akiko KOBAYASHI ◽  
Shinichi OHASHI ◽  
Motoaki SANO
Keyword(s):  
Aspergillus Oryzae ◽  
Kojic Acid ◽  
Acid Synthesis

scholarly journals Cleaning the Cellular Factory–Deletion of McrA in Aspergillus oryzae NSAR1 and the Generation of a Novel Kojic Acid Deficient Strain for Cleaner Heterologous Production of Secondary Metabolites

2021 ◽  
Vol 2 ◽  
Author(s):  
Trong T. Dao ◽  
Kate M. J. de Mattos-Shipley ◽  
Ian M. Prosser ◽  
Katherine Williams ◽  
Marija K. Zacharova ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Aspergillus Oryzae ◽  
Kojic Acid ◽  
Negative Regulator ◽  
Secondary Metabolite Production ◽  
Phenotypic Change ◽  
Metabolite Production ◽  
Host Strains

The use of filamentous fungi as cellular factories, where natural product pathways can be refactored and expressed in a host strain, continues to aid the field of natural product discovery. Much work has been done to develop host strains which are genetically tractable, and for which there are multiple selectable markers and controllable expression systems. To fully exploit these strains, it is beneficial to understand their natural metabolic capabilities, as such knowledge can rule out host metabolites from analysis of transgenic lines and highlight any potential interplay between endogenous and exogenous pathways. Additionally, once identified, the deletion of secondary metabolite pathways from host strains can simplify the detection and purification of heterologous compounds. To this end, secondary metabolite production in Aspergillus oryzae strain NSAR1 has been investigated via the deletion of the newly discovered negative regulator of secondary metabolism, mcrA (multicluster regulator A). In all ascomycetes previously studied mcrA deletion led to an increase in secondary metabolite production. Surprisingly, the only detectable phenotypic change in NSAR1 was a doubling in the yields of kojic acid, with no novel secondary metabolites produced. This supports the previous claim that secondary metabolite production has been repressed in A. oryzae and demonstrates that such repression is not McrA-mediated. Strain NSAR1 was then modified by employing CRISPR-Cas9 technology to disrupt the production of kojic acid, generating the novel strain NSARΔK, which combines the various beneficial traits of NSAR1 with a uniquely clean secondary metabolite background.

Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis

2019 ◽  
Vol 42 (5) ◽  
pp. 753-761 ◽  
Author(s):  
Weimeng Feng ◽  
Jinrui Liang ◽  
Bingbing Wang ◽  
Jianhua Chen
Keyword(s):  
Mutant Strain ◽  
Aspergillus Oryzae ◽  
Acid Production ◽  
Kojic Acid ◽  
Kojic Acid Production ◽  
Combined Mutagenesis

Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484

1998 ◽  
Vol 85 (5) ◽  
pp. 488-494 ◽  
Author(s):  
Yasushi Wakisaka ◽  
Takayuki Segawa ◽  
Koreyoshi Imamura ◽  
Takaharu Sakiyama ◽  
Kazuhiro Nakanishi
Keyword(s):  
Aspergillus Oryzae ◽  
Liquid Culture ◽  
Kojic Acid ◽  
Membrane Surface ◽  
Surface Liquid
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