Long-term culture of keratinocyte-like cells derived from mouse embryonic stem cells

2008 ◽  
Vol 44 (7) ◽  
pp. 193-203 ◽  
Author(s):  
Hai-Jun Huang ◽  
Qi-Shuang Gao ◽  
Bi-Fei Tao ◽  
Si-Wen Jiang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Long Term Culture

scholarly journals Assessment of Stem Cell Markers During Long-Term Culture of Mouse Embryonic Stem Cells

2004 ◽  
Vol 44 (1/2) ◽  
pp. 77-91 ◽  
Author(s):  
A. Berrill ◽  
H.L. Tan ◽  
S.C. Wuang ◽  
W.J. Fong ◽  
Andre B.H. Choo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Long Term Culture

Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix

2006 ◽  
Vol 142 (1) ◽  
pp. 119-122 ◽  
Author(s):  
I. I. Selezneva ◽  
I. V. Savintseva ◽  
E. F. Vikhlyantseva ◽  
G. A. Davydova ◽  
B. K. Gavrilyuk
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Chitosan Gel

Tumor progression of culture-adapted human embryonic stem cells during long-term culture

2008 ◽  
Vol 47 (8) ◽  
pp. 665-679 ◽  
Author(s):  
Sheng Yang ◽  
Ge Lin ◽  
Yue-Qiu Tan ◽  
Di Zhou ◽  
Lei-Yu Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Tumor Progression ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Long Term Culture

Long-term culture of human embryonic stem cells in feeder-free conditions

2004 ◽  
Vol 229 (2) ◽  
pp. 259-274 ◽  
Author(s):  
Elen S. Rosler ◽  
Gregory J. Fisk ◽  
Ximena Ares ◽  
John Irving ◽  
Takumi Miura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Long Term Culture

scholarly journals Inhibition of β-catenin–TCF1 interaction delays differentiation of mouse embryonic stem cells

2015 ◽  
Vol 211 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Sujash S. Chatterjee ◽  
Abil Saj ◽  
Tenzin Gocha ◽  
Matthew Murphy ◽  
Foster C. Gonsalves ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Fate ◽  
Protein Interactions ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Protein Protein Interactions ◽  
Nanog Expression ◽  
Context Specific

The ability of mouse embryonic stem cells (mESCs) to self-renew or differentiate into various cell lineages is regulated by signaling pathways and a core pluripotency transcriptional network (PTN) comprising Nanog, Oct4, and Sox2. The Wnt/β-catenin pathway promotes pluripotency by alleviating T cell factor TCF3-mediated repression of the PTN. However, it has remained unclear how β-catenin’s function as a transcriptional activator with TCF1 influences mESC fate. Here, we show that TCF1-mediated transcription is up-regulated in differentiating mESCs and that chemical inhibition of β-catenin/TCF1 interaction improves long-term self-renewal and enhances functional pluripotency. Genetic loss of TCF1 inhibited differentiation by delaying exit from pluripotency and conferred a transcriptional profile strikingly reminiscent of self-renewing mESCs with high Nanog expression. Together, our data suggest that β-catenin’s function in regulating mESCs is highly context specific and that its interaction with TCF1 promotes differentiation, further highlighting the need for understanding how its individual protein–protein interactions drive stem cell fate.

scholarly journals Hierarchical Micro-Nano Surface Topography Promotes Long-Term Maintenance of Undifferentiated Mouse Embryonic Stem Cells

Nano Letters ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 7146-7154 ◽  
Author(s):  
Mona Jaggy ◽  
Ping Zhang ◽  
Alexandra M. Greiner ◽  
Tatjana J. Autenrieth ◽  
Victoria Nedashkivska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Surface Topography ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Term Maintenance

scholarly journals Derivation and long-term culture of human parthenogenetic embryonic stem cells using human foreskin feeders

2010 ◽  
Vol 27 (6) ◽  
pp. 285-291 ◽  
Author(s):  
Zhenyu Lu ◽  
Wanwan Zhu ◽  
Yang Yu ◽  
Dan Jin ◽  
Yunqian Guan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Long Term Culture
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