Fibroblast movements during contraction of collagen lattices—A quantitative study using a new three-dimensional time-lapse technique with phase-contrast laser scanning microscopy

1998 ◽  
Vol 34 (8) ◽  
pp. 640-645 ◽  
Author(s):  
Erkki Tarpila ◽  
Reza M. Ghassemifar ◽  
Lennart E. Franzén
Keyword(s):  
Quantitative Study ◽  
Phase Contrast ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Time Lapse ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Collagen Lattices

Behaviour of nucleolar proteins in nuclei lacking ribosomal genes. A study by confocal laser scanning microscopy

1991 ◽  
Vol 98 (1) ◽  
pp. 99-105
Author(s):  
D. Hernandez-Verdun ◽  
M. Robert-Nicoud ◽  
G. Geraud ◽  
C. Masson
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Specific Marker ◽  
Nucleolar Proteins ◽  
Fibrillar Component

The behaviour of nucleolar proteins in cycling PtK1 cells and in micronuclei with or without NORs was investigated by immunofluorescence using antibodies from autoimmune sera and confocal laser scanning microscopy. These antibodies were shown by electron microscopy to recognize antigens confined to only one of the three basic nucleolar components: fibrillar centres (FC), dense fibrillar component (DFC) and granular component (GC). Serial optical sections allowed us to determine the three-dimensional organization of these components in the nucleolus of cycling cells. Furthermore, clear differences were found in the distribution of the various antigens in micronucleated cells. Three patterns could be observed: (1) the FC antigens were found mainly in the nucleoli, but also in varying amounts in the dots; (2) surprisingly, the DFC antigens were found to accumulate preferentially in the dots; (3) the GC-specific marker stained intensively the nucleoli as well the dots. The results are interpreted with regard to possible mechanisms for targeting nucleolar proteins to the site of nucleolar formation.

scholarly journals Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy

2013 ◽  
Vol 251 (1) ◽  
pp. 14-18 ◽  
Author(s):  
A.P.M. ANTUNES ◽  
A.D. COVINGTON ◽  
N. PETFORD ◽  
T. MURRAY ◽  
D. WERTHEIM
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Skin Structure

Rayburst sampling, an algorithm for automated three-dimensional shape analysis from laser scanning microscopy images

2006 ◽  
Vol 1 (4) ◽  
pp. 2152-2161 ◽  
Author(s):  
Alfredo Rodriguez ◽  
Douglas B Ehlenberger ◽  
Patrick R Hof ◽  
Susan L Wearne
Keyword(s):  
Shape Analysis ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Scanning Microscopy ◽  
Dimensional Shape ◽  
Three Dimensional Shape ◽  
Microscopy Images

Toxicity Evaluation of Two Dental Composites: Three-Dimensional Confocal Laser Scanning Microscopy Time-Lapse Imaging of Cell Behavior

2013 ◽  
Vol 19 (3) ◽  
pp. 596-607 ◽  
Author(s):  
Ghania Nina Attik ◽  
Nelly Pradelle-Plasse ◽  
Doris Campos ◽  
Pierre Colon ◽  
Brigitte Grosgogeat
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Cell Metabolism ◽  
Three Dimensional ◽  
Time Lapse ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Dental Composites ◽  
Confocal Laser ◽  
Time Lapse Imaging

AbstractThe purpose of this study was to investigate thein vitrobiocompatibility of two dental composites (namely A and B) with similar chemical composition used for direct restoration using three-dimensional confocal laser scanning microscopy (CLSM) time-lapse imaging. Time-lapse imaging was performed on cultured human HGF-1 fibroblast-like cells after staining using Live/Dead®. Image analysis showed a higher mortality rate in the presence of composite A than composite B. The viability rate decreased in a time-dependent manner during the 5 h of exposure. Morphological alterations were associated with toxic effects; cells were enlarged and more rounded in the presence of composite A as shown by F-actin and cell nuclei staining. Resazurin assay was used to confirm the active potential of composites in cell metabolism; results showed severe cytotoxic effects in the presence of both no light-curing composites after 24 h of direct contact. However, extracts of polymerized composites induced a moderate decrease in cell metabolism after the same incubation period. Composite B was significantly better tolerated than composite A at all investigated end points and all time points. The finding confirmed that the used CLSM method was sufficiently sensitive to differentiate the biocompatibility behavior of two composites based on similar methacrylate monomers.

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