Improvement of English walnut somatic embryo germination and conversion by desiccation treatments and plantlet development by lower medium salts

2000 ◽  
Vol 36 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Haoru Tang ◽  
Zhenglong Ren ◽  
Gari Krczal
Keyword(s):  
Somatic Embryo ◽  
Embryo Germination ◽  
Somatic Embryo Germination ◽  
English Walnut ◽  
Lower Medium

Effects of Polyethylene Glycol on Development of Grape (Vitis vinifera L. `Thompson Seedless') Somatic Embryos

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 557c-557
Author(s):  
Fred K. Westphal ◽  
Michael E. Compton
Keyword(s):  
Polyethylene Glycol ◽  
Somatic Embryo ◽  
Somatic Embryos ◽  
Vitis Vinifera L ◽  
Zygotic Embryos ◽  
Embryo Germination ◽  
Thompson Seedless ◽  
Germination Medium ◽  
Peg Treatment ◽  
Somatic Embryo Germination

Torpedo-stage somatic embryos were selected from actively growing cultures and trasferred to embryo maintenance medium [MS with (per liter) 412.5 mg NH4NO3, 475 mg KNO3, 1 g myo-inositol, 90 g sucrose, 2 g activated charcoal, and 7 g TC agar] supplemented with either 0%, 2.5%, 5%, 7.5%, or 10% polyethylene glycol (PEG) for 4, 8, or 12 weeks. Embryos placed on treatment media were transferred directly to grape somatic embryo germination medium [MS with (per liter) 1 g myo-inositol, 30 g sucrose, 1 M benzyladenine, and 7 g TC agar] once their PEG treatment was terminated. The number of embryos that germinated was recorded 4 weeks after transfer to somatic embryo germination medium. The number of germinated embryos that differentiated into plants was recorded at 8 weeks. There was no difference in germination rates and embryo differentiation among embryos incubated on medium with or without PEG for 4 weeks. A difference in embryo growth rate was observed after 8 weeks on medium with PEG. Embryo grew fastest on media containing 5% or 7.5% PEG. In addition, embryos grown on medium with 5% or 7.5% PEG were morphologically similar to zygotic embryos.

scholarly journals Metabolome and transcriptome profiling reveal new insights into somatic embryo germination in Norway spruce (Picea abies)

2017 ◽  
Vol 37 (12) ◽  
pp. 1752-1766 ◽  
Author(s):  
Izabela Dobrowolska ◽  
Edward Businge ◽  
Ilka N Abreu ◽  
Thomas Moritz ◽  
Ulrika Egertsdotter
Keyword(s):  
Picea Abies ◽  
Somatic Embryo ◽  
Norway Spruce ◽  
Transcriptome Profiling ◽  
Embryo Germination ◽  
Somatic Embryo Germination

scholarly journals Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines

Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1592
Author(s):  
Momi Tsuruta ◽  
Tsuyoshi E. Maruyama ◽  
Saneyoshi Ueno ◽  
Takumi Kaneeda ◽  
Yoshinari Moriguchi
Keyword(s):  
Growth Performance ◽  
Somatic Embryo ◽  
Japanese Cedar ◽  
In Vitro Growth ◽  
Embryo Germination ◽  
Embryogenic Cell ◽  
Somatic Embryo Maturation ◽  
Embryogenic Cell Lines ◽  
Somatic Embryo Germination

With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilities during somatic embryogenesis, initial in vitro growth traits, and subsequent growth of somatic seedlings. In the present study, we provide the first basic information on somatic embryo maturation efficiency, somatic embryo germination, and plantlet conversion frequencies, as well as on in vitro growth performance of pollen-free somatic plantlets derived from different embryogenic cell lines (ECLs). Somatic embryo maturation efficiency varied from 34 to 514 cotyledonary embryos per plate and the average for the 19 ECLs tested was 244 embryos per plate. Subsequently, the overall average rates of somatic embryo germination and conversion among ECLs were 87.8% and 85.3%, respectively. The results of in vitro growth performance of pollen-free somatic plantlets showed significant differences in growth rate among ECLs.

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