Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus  praecox ssp. orientalis) plants at the flowering stage

The expression of GUS and ipt genes under control of a chalcone synthase (chs) promoter (PCHS) has been determined in tobacco (Nicotiana tabacum L.) plants and related to the development of plants expressing the chimaeric PCHS -ipt gene. GUS gene expression, which served as a model for the expression of the ipt gene, was highest in the internal phloem tissue of stems, in mature leaf laminae and in the upper part of corollas when fully open. Expression of the PCHS -ipt gene was assessed by quantifying the cytokinins produced, by determining incorporation of [3H]adenine into cytokinins and by quantifying ipt mRNA. Results from these studies were in general agreement with those based on expression of the PCHS -GUS gene. The chs promoter controlled expression of the ipt gene with some degree of tissue and temporal specificity. Expression of the ipt gene markedly elevated the cytokinin level in mature leaf laminae and the upper stems of flowering plants. The former was associated with retardation of leaf senescence and increased rates of transpiration due to changes in number, size and aperture of stomata, while the latter was associated with development of lateral shoots. In shoot tip cultures, 2-fold elevations in endogenous cytokinin level caused clear changes in development and this is discussed in relation to current concepts concerning the hormonal control of plant development. Using the transgenic tobacco tissues, it was shown that cis-zeatin is a substrate for cytokinin oxidase, that cis-zeatin is not converted to trans-zeatin in these tissues and that the endogenous cytokinin level influences the level of cytokinin oxidase activity in tissue and the rate of degradation of exogenous zeatin riboside to adenosine.

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Transient GUS gene expression in Brassica napus electroporated microspores

Plant Science ◽

10.1016/0168-9452(93)90047-4 ◽

1993 ◽

Vol 93 (1-2) ◽

pp. 177-184 ◽

Cited By ~ 30

Author(s):

Marie-Françoise Jardinaud ◽

André Souvré ◽

Gilbert Alibert

Keyword(s):

Gene Expression ◽

Brassica Napus ◽

Gus Gene ◽

Gus Gene Expression

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STUDI PERBANDINGAN METODE TRANSFORMASI DNA MENGGUNAKAN VEKTOR Agrobacterium tumefaciens PADA TANAMAN TEBU (Sacharum hybrid)

Journal of Biological Researches ◽

10.23869/bphjbr.13.1.20076 ◽

2007 ◽

Vol 13 (1) ◽

pp. 39-44

Author(s):

Sri Setyati ◽

Purnama Oktaviandari ◽

Muhammad Hazmi ◽

Bambang Sugiharto

Keyword(s):

Gene Expression ◽

Embryogenic Callus ◽

Multiple Shoots ◽

Suspension Cultures ◽

Gus Gene ◽

In Vitro Plants ◽

Histochemical Observation ◽

Selection Medium ◽

Gus Gene Expression

In order to compare transient expression of gus gene driven by CaMV 35S and rice ubiquitin RUBQ2 promoters, a DNA transformation was conducted using embryogenic callus and suspension cultures of sugarcane. The transient gus expression was observed by histochemical staining method. The histochemical observation of GUS activity after co-cultivation showed that RUBQ2 promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. The suspension cultures slightly increased transient gus gene expression compared to embryogenic callus. However, the histochemical analysis of regenerated putative transformant plants after 5 successive cycles on the selection medium showed no blue spots of gus gene expression. PCR amplification of DNA for CaMV35 or nptII in putative transformant plants confirmed that there was no integration of the transformed gene in the genome DNA. The results suggested a possibility of somaclonal variation with callus propagation, thus did not produce transformed plants. To avoid the somaclonal variation, the transformation was conducted using in vitro plants and multiple shoots without intervening callus phase. Histochemical observation of infected materials after co-cultivation showed that almost all of the infected materials partially exhibited blue color in the basal region. In case of in vitro plants, they rapidly grow and multiplied in the selection medium, thus the method provided an excellent system for the transformation in sugarcane. The results suggest that in vitro plants as well as multiple shoots need further investigation to be used as target tissues for Agrobacteriummediated transformation in sugarcane.

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GUS Gene expression and plant regeneration via somatic embryogenesis in cucumber (Cucumis sativus L.)

Journal of Plant Biotechnology ◽

10.5010/jpb.2008.35.4.275 ◽

2008 ◽

Vol 35 (4) ◽

pp. 275-280 ◽

Cited By ~ 5

Author(s):

Hyun-A Kim ◽

Boo-Youn Lee ◽

Jin-Jung Jeon ◽

Dong-Woog Choi ◽

Pil-Son Choi ◽

...

Keyword(s):

Gene Expression ◽

Somatic Embryogenesis ◽

Plant Regeneration ◽

Cucumis Sativus ◽

Gus Gene ◽

Cucumis Sativus L ◽

Gus Gene Expression

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Expression and Promoter Analysis of Six Heat Stress-Inducible Genes in Rice

The Scientific World JOURNAL ◽

10.1155/2013/397401 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 19

Author(s):

Wirat Rerksiri ◽

Xianwen Zhang ◽

Hairong Xiong ◽

Xinbo Chen

Keyword(s):

Gene Expression ◽

Heat Stress ◽

Cold Treatment ◽

Evolutionary Process ◽

High Heat ◽

Microarray Data Analysis ◽

Pcr Analysis ◽

Gus Gene ◽

Gus Gene Expression ◽

Inducible Genes

During the long evolutionary process, plant gradually formed a series of strategies and mechanisms to cope with stress environment such as drought, heat, cold, and high salinity. Six highly heat responsive genes were identified in rice by microarray data analysis. The qRT-PCR analysis confirmed that the expression of these six genes were highly heat inducible and moderately responded to salt stress, polyethylene glycol, and abscisic acid treatment, but little affected by cold treatment. Promoters of the three highly heat-inducible genes (OsHsfB2cp, PM19p, and Hsp90p) were used to drive GUS gene expression in rice. The results of the GUS gene expression, histochemical staining, and GUS activities in panicles and flag leaves of the transgenic rice plants confirmed high heat-induced GUS activities and moderate drought-induced activities. The three promoters exhibited similar high activity lever in rice leaf under heat, but OsHsfB2cp and PM19p showed much higher activities in panicles under heat stress. Our work confirmed that the OsHsfB2c and PM19 promoters were highly heat inducible and further characterization and reconstruction ofcis-elements in their promoters could lead to the development of highly effective heat-inducible promoters for plant genetic engineering.

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