Gibberellic acid improved shoot multiplication in Cephaelis ipecacuanha

Multiple adventitious shoot formed from internodal segments of Cephaelis ipecacuanha cultured 25 days on Gamborg basal medium (GAMBORG et al., 1968) supplemented with 6.66mM 6-benzylaminopurine there was a maximum of nine shoots per segment and an average of five shoots per segment formed. The presence of gibberellic acid in the subculture media promoted shoot elongation in all treatments. The shoots attained 3cm in height and rooting of 100% after 35 days of culturing upon Murashige and Skoog's basal medium (MS), added with 4.92mM indole-3-butyric acid, 0.87m gibberellic acid and 0.1% activated charcoal. Further growth was accelerated after the transfer to 1/2 MS without growth regulators. Rooted plantlets transferred to potting soil could be successfully established.

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Micropropagation of Two Selected Male Kiwifruit and Analysis of Genetic Variation with AFLP Markers

HortScience ◽

10.21273/hortsci.40.3.740 ◽

2005 ◽

Vol 40 (3) ◽

pp. 740-746 ◽

Cited By ~ 8

Author(s):

M.J. Prado ◽

M.T. Herrera ◽

R.A. Vázquez ◽

S. Romo ◽

M.V. González

Keyword(s):

Genetic Variation ◽

Gibberellic Acid ◽

Shoot Multiplication ◽

Aflp Markers ◽

Naphthaleneacetic Acid ◽

Multiplication Rate ◽

Variation Analysis ◽

Half Strength ◽

Species Being ◽

Regenerated Plantlets

A simple and reliable protocol for micropropagation during 12 subcultures of two field growth male plants of kiwifruit [Actinidia deliciosa (A.Chev.) Liang and Ferguson] is described. The best results of shoot multiplication and elongation were obtained in Cheng's K(h) medium in the presence of 0.5 μm NAA, 22 μm BA and 1.4 μm GA3 for `Tomuri' explants, and of 0.1 μm NAA, 4.4 μm BA, and 0.3 μm GA3 for clone A explants. In addition, the cytokinin compounds TDZ and mT were also tested allowing improving the multiplication rate in `Tomuri' explants. For rooting, `Tomuri' and clone A developed shoots were treated by basal immersion in a 5 mm IBA solution for 15 seconds. Treated shoots were then cultured in half-strength K(h) medium without growth regulators showing 100% rooting after 30 days. Regenerated plantlets were successfully transplanted to soil (90% survival) and they are actively growing in the field. Somaclonal variation analysis by AFLP was carried out using 15 primer combinations, yielding reproducible and well-resolved bands with a 57% of polymorphism. AFLP markers showed to be effective to discriminate genetic variation in this species, being greater in clone A than `Tomuri'. Chemical names used: N6-benzyladenine (BA); gibberellic acid (GA3); indole-3-butyric acid (IBA); meta-topolin (mT); naphthaleneacetic acid (NAA); thidiazuron (TDZ).

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Gibberellic acid promotes Atriplex shoot multiplication and elongation

Plant Science Letters ◽

10.1016/0304-4211(80)90169-8 ◽

1980 ◽

Vol 17 (3) ◽

pp. 363-369 ◽

Cited By ~ 14

Author(s):

Zachary S. Wochok ◽

Carolyn J. Sluis

Keyword(s):

Gibberellic Acid ◽

Shoot Multiplication

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Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L.

Plant Growth Regulation ◽

10.1007/s10725-010-9505-6 ◽

2010 ◽

Vol 62 (2) ◽

pp. 181-188 ◽

Cited By ~ 1

Author(s):

Radomirka Nikolić ◽

Nevena Mitić ◽

Slavica Ninković ◽

Branka Vinterhalter ◽

Snežana Zdravković-Korać ◽

...

Keyword(s):

Gibberellic Acid ◽

In Vitro Regeneration ◽

Shoot Multiplication ◽

Lotus Corniculatus

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Micrografting Compatibility Between Two Almond Cultivars and Four Wild Species

Basic and Applied Sciences - Scientific Journal of King Faisal University ◽

10.37575/b/agr/0057 ◽

2021 ◽

Vol 22 (1) ◽

pp. 1-6

Author(s):

Wafaa Koaym ◽

Mohamed Battha ◽

Mhasen Twaklna

Keyword(s):

Gibberellic Acid ◽

Growth Regulators ◽

Shoot Multiplication ◽

Prunus Dulcis ◽

Shoot Length ◽

Ms Medium ◽

Success Rates ◽

Benzyl Adenine ◽

High Quality ◽

Wild Almond

Micropropagation and micrografting techniques are important methods used to obtain high quality plants. This research aimed to investigate the effect of wild almond species (Prunus communis, P. orientalis, P. korschinskii, and P. arabica) on the success of micrografting almond cultivars (Prunus dulcis cvs. Shami Furk and Dafadii)and determine which combination of growth regulators lead to the highest rate of multiplication in micrografted cultivars. The shoot tips were grafted onto the rooted rootstocks by inverted T-budding. The results indicated that Murashige and Skoog (MS) medium, supplemented with 1 mg/l benzyl adenine (BA), 0.1 mg/l indole-3-butyric acid (IBA) and 0.2mg/l gibberellic acid (GA3), achieved the highest shoot multiplication with an average of 5.31 and 3.67 shoots per explant and an average of 6.23cm and 4.98cm shoot length in cultivars Shami Furk and Dafadii, respectively. The highest grafting success rates were 80% and 74.26% obtained from Shami Furk/P. arabica and Dafadii/P. arabica combinations, respectively, while the lowest success rate was 50.63% with the Dafadii/P. orientalis combination. The liquid MS medium supplemented with 0.5 mg/l BA + 0.1 mg/l IBA achieved the highest micrografting success and scion shoot length. This research can be used to improve almond cultivation.

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