Isolation and characterization of the aureobasidin A-resistant gene, aur1 R , on Schizosaccharomyces pombe : roles of Aur1p + in cell morphogenesis

1998 ◽  
Vol 33 (1) ◽  
pp. 38-45 ◽  
Author(s):  
T. Hashida-Okado ◽  
R. Yasumoto ◽  
Masahiro Endo ◽  
Kazutoh Takesako ◽  
Ikunoshin Kato
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Cell Morphogenesis ◽  
Resistant Gene ◽  
Isolation And Characterization ◽  
Aureobasidin A

Isolation and characterization of Schizosaccharomyces pombe mutants with defective NAD-dependent malic enzyme

1986 ◽  
Vol 32 (6) ◽  
pp. 481-486 ◽  
Author(s):  
C. Osothsilp ◽  
R. E. Subden
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Malic Enzyme ◽  
Pool Analysis ◽  
Starch Gel Electrophoresis ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Starch Gel ◽  
Colony Color ◽  
Color Indicator

To obtain NAD-dependent malic enzyme mutants of Schizosaccharomyces pombe, a colony color indicator screening system was developed. Mutants defective for malic acid utilization (mau mutants) are yellow, while wild-type colonies are blue on the defined bromcresol green based indicator medium. NAD-dependent malic enzyme mutants were distinguished from other mau mutants by subsequent, starch gel electrophoresis, spectrophotometry, complementation tests, and intermediate pool analysis with cell-free extracts.

Isolation and characterization of the Schizosaccharomyces pombe rad3 gene, involved in the DNA damage and DNA synthesis checkpoints

Gene ◽  
1992 ◽  
Vol 119 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Brent L. Seaton ◽  
Jennifer Yucel ◽  
Per Sunnerhagen ◽  
Suresh Subramani
Keyword(s):  
Dna Damage ◽  
Dna Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Isolation And Characterization

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals Isolation and characterization of Schizosaccharomyces pombe cut mutants that block nuclear division but not cytokinesis

1986 ◽  
Vol 5 (11) ◽  
pp. 2973-2979 ◽  
Author(s):  
Tatsuya Hirano ◽  
Shin-ichi Funahashi ◽  
Tadashi Uemura ◽  
Mitsuhiro Yanagida
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Nuclear Division ◽  
Isolation And Characterization

Isolation and characterization of SpTRK, a gene from schizosaccharomyces pombe predicted to encode a K+ transporter protein

Gene ◽  
1995 ◽  
Vol 161 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Viatcheslav A. Soldatenkov ◽  
Juan A. Velasco ◽  
Matias A. Avila ◽  
Anatoly Dritschilo ◽  
Vicente Notario
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Transporter Protein ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document