Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey

Isoëtes sabatina is an aquatic quillwort endemic to Italy. It is one of the rarest quillworts in Europe, and is critically endangered due to restricted range and to the continuous decline of both population and habitat quality. This study aims to develop an optimized protocol to reproduce and grow I. sabatina sporelings. Mature and immature megaspores were mixed with mature microspores to evaluate the influence of the developmental stage on germination and sporeling development. Two substrates, distilled water and water-agar medium, were tested for germination and sporeling emergence, and three substrates, sand, lake sediment and water-agar, were tested for transplants. A high percentage of megaspore germination (a total of 79.1%) was obtained in both substrates, higher for mature than immature spores. A total of 351 sporelings were produced in distilled water and water-agar cultures, with similar percentages (64.5% and 69.6%, respectively). The development stage of the megaspores affected both germination and sporeling development. Sporeling emergence showed significantly higher percentages in mature megaspores than immature ones (69.6% vs. 11.6%, respectively), with 85% of germinated spores developing sporelings. Only transplants over water-agar medium were successful. This protocol could be useful for the propagation of sporelings as the key step towards the planning of in situ actions to save this Mediterranean quillwort from extinction.

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In vitro propagation and assessment of genetic uniformity along with chemical characterization in Hildegardia populifolia (Roxb.) Schott & Endl.: a critically endangered medicinal tree

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-020-10085-w ◽

2020 ◽

Vol 56 (6) ◽

pp. 803-816 ◽

Cited By ~ 2

Author(s):

Anamica Upadhyay ◽

Anwar Shahzad ◽

Zishan Ahmad

Keyword(s):

In Vitro Propagation ◽

Chemical Characterization ◽

Critically Endangered ◽

Medicinal Tree ◽

Genetic Uniformity

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Efficient In Vitro Propagation Protocol of Swertia chirayita (Roxb. ex Fleming) Karsten: A Critically Endangered Medicinal Plant

National Academy Science Letters ◽

10.1007/s40009-018-0624-3 ◽

2018 ◽

Vol 41 (2) ◽

pp. 123-127 ◽

Cited By ~ 3

Author(s):

Rajeev Ranjan Kumar ◽

Vijay Kant Purohit ◽

P. Prasad ◽

Anant Ram Nautiyal

Keyword(s):

Medicinal Plant ◽

In Vitro Propagation ◽

Critically Endangered ◽

Swertia Chirayita ◽

Endangered Medicinal Plant

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Development of an in vitro propagation protocol for ex situ conservation of two critically endangered species of Commersonia (Malvaceae) from Western Australia

Australian Journal of Botany ◽

10.1071/bt10184 ◽

2010 ◽

Vol 58 (7) ◽

pp. 565 ◽

Cited By ~ 3

Author(s):

Shahab Nikabadi ◽

Eric Bunn ◽

Shane Turner ◽

Jason Stevens ◽

Kingsley Dixon

Keyword(s):

Endangered Species ◽

Incubation Period ◽

Western Australia ◽

In Vitro Propagation ◽

Leaf Explants ◽

Leaf Explant ◽

Critically Endangered ◽

Ms Medium ◽

Critically Endangered Species

Protocols for in vitro propagation of two critically endangered species, Commersonia adenothalia C.F.Wilkins ms and Commersonia sp. Mt Groper (R. Cranfield & D. Kabay 9157), from south-western Western Australia were established utilising both shoot and in vitro leaf explants. Regeneration from leaf explants was highest, with an average of four shoots per leaf explant per a 4-week incubation period on ½-strength Murashige and Skoog (MS) medium with 2.5 µM thidiazuron (TDZ) + 2.5 µM 3-indoleacetic acid (IAA) for C. adenothalia and 13 shoots per leaf explant on ½-strength MS medium + 4.5 µM 6-benzylaminopurine (BAP) and 2.5 µM 1-naphthaleneacetic acid (NAA) for C. sp. Mt Groper. Shoot proliferation using single shoot explants of C. adenothalia resulted in a maximum average of 3.5 shoots per shoot explant per a 5-week incubation period on ½-strength MS medium + 5 µM kinetin and 0.5 µM BAP, whereas maximum mean shoot multiplication with C. sp. Mt Groper (×30 shoots per shoot explant per a 5-week incubation period) was recorded with ½-strength MS medium + 2.5 µM kinetin and 1 µM BAP. In general, C. sp. Mt Groper was much more reactive to cytokinins than was C. adenothalia, with prolific regeneration of shoots from leaf explants or shoot explants. Both species produced roots readily on ½-strength MS medium without added hormones or with 5 µM indole-3-butyric acid (IBA) (100% rooting in 3–4 weeks) and rooted plantlets survived the transition to soil (~70% survival).

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