In vitro propagation and field establishment of Eulophia cullenii (Wight) Bl., a critically endangered orchid of Western Ghats, India through culture of seeds and axenic seedling-derived rhizomes

The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

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Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey

In Vitro Cellular & Developmental Biology - Plant ◽

10.1007/s11627-020-10082-z ◽

2020 ◽

Vol 56 (6) ◽

pp. 911-914

Author(s):

Çiğdem Işıkalan ◽

Pınar Orcan ◽

Filiz Akbaş ◽

Süreyya Namlı ◽

İbrahim Selçuk Kuru ◽

...

Keyword(s):

In Vitro Propagation ◽

Critically Endangered

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Evaluation of in vitro antioxidant activity, HPLC and GC–MS analysis along with chemoprofiling of Decalepis arayalpathra: a critically endangered plant of Western Ghats, India

RENDICONTI LINCEI ◽

10.1007/s12210-017-0645-8 ◽

2017 ◽

Vol 28 (4) ◽

pp. 711-720 ◽

Cited By ~ 2

Author(s):

Ahmad Zishan ◽

Shahzad Anwar ◽

Sharma Shiwali

Keyword(s):

Antioxidant Activity ◽

Western Ghats ◽

Critically Endangered ◽

Endangered Plant ◽

Ms Analysis ◽

Decalepis Arayalpathra ◽

In Vitro Antioxidant Activity

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In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-019-0003-6 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 2

Author(s):

Avinash A. Adsul ◽

Jaykumar J. Chavan ◽

Nikhil B. Gaikwad ◽

Rajaram V. Gurav ◽

Ghansham B. Dixit ◽

...

Keyword(s):

Butyric Acid ◽

Western Ghats ◽

Large Scale ◽

In Vitro Regeneration ◽

Critically Endangered ◽

Ms Medium ◽

Regeneration System ◽

The Western Ghats ◽

Flowering In Vitro

Abstract The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.

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