Effect of light intensity on in vitro introduction and multiplication of Eucalyptus grandis × Eucalyptus urophylla

2021 ◽  
Author(s):  
Denys Matheus Santana Costa Souza ◽  
Sérgio Bruno Fernandes ◽  
Eduardo Oliveira Silva ◽  
Vinícius Politi Duarte ◽  
Douglas Santos Gonçalves ◽  
...  
Keyword(s):  
Light Intensity ◽  
Eucalyptus Grandis ◽  
Eucalyptus Urophylla ◽  
Effect Of Light

scholarly journals IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

2017 ◽  
Vol 41 (6) ◽  
Author(s):  
Ricardo Gallo ◽  
Aloisio Xavier ◽  
Luciana Coelho de Moura ◽  
Brener de Almeida Oliveira ◽  
Heloisa Rocha do Nascimento ◽  
...  
Keyword(s):  
Eucalyptus Grandis ◽  
Stem Diameter ◽  
Residual Effects ◽  
Seedling Height ◽  
Seedling Vigor ◽  
Eucalyptus Urophylla ◽  
Ex Vitro ◽  
Semisolid Medium ◽  
Hybrid Clones

ABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucalyptus grandis x E. urophylla clone and every 30 days for Eucalyptus urophylla x E. globulus clone. The following variables were evaluated under in vitro conditions: number of shoots > 0.5 cm, number of microcuttings > 2 cm, length of the longest microcutting, and shoots vigor. Under ex vitro conditions, in the greenhouse and shade house, the following variables were evaluated: seedling height, percentage of survival, stem diameter, percentage of root observed at the lower end of the tube, and seedling vigor. In full sun (ex vitro), the following variables were analyzed: seedling height, stem diameter, survival, number of roots, root volume, seedling vigor, and shoot and root dry matter. Good in vitro microcuttings productivity was observed over the successive collections. IBA levels were adjusted for each clone, ranging from 0.25 to 0.50 mg L-1 for Eucalyptus grandis x E. urophylla clone, and from 0.75 to 1.0 mg L-1 for Eucalyptus urophylla x E. globulus clone. IBA concentrations led to residual effects under ex vitro conditions, providing good rooting and survival for Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones at IBA concentrations between 0.25 and 0.50 mg L-1 and between 0.50 and 1.0 mg L-1, respectively.

In vitro rooting of genetically related Eucalyptus urophylla × Eucalyptus grandis clones in relation to the time spent in culture

Trees ◽  
2009 ◽  
Vol 23 (5) ◽  
pp. 931-940 ◽  
Author(s):  
François Mankessi ◽  
Aubin Saya ◽  
Christelle Baptiste ◽  
Sophie Nourissier ◽  
Olivier Monteuuis
Keyword(s):  
Eucalyptus Grandis ◽  
In Vitro Rooting ◽  
Eucalyptus Urophylla

Effect of light intensity on in vitro regeneration in some relict endemic species of the Crimean flora

2021 ◽  
pp. 27-34
Author(s):  
O.V. Mitrofanova ◽  
N.N. Ivanova ◽  
N.P. Lesnikova-Sedoshenko ◽  
V.A. Brailko ◽  
I.V. Zhdanova ◽  
...  
Keyword(s):  
Light Intensity ◽  
Endemic Species ◽  
In Vitro Regeneration ◽  
Effect Of Light

scholarly journals Organogênese in vitro a partir de explante caulinar na regeneração de clones de Eucalyptus grandis W. Hill ex Maiden X E. urophylla S. T. Blake

2004 ◽  
Vol 28 (5) ◽  
pp. 643-653 ◽  
Author(s):  
Elisa Cristina Soares de Carvalho Alves ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni
Keyword(s):  
Eucalyptus Grandis ◽  
Eucalyptus Urophylla

Com o objetivo de testar a regeneração in vitro por organogênese a partir de explante caulinar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla, foram avaliados os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tiadiazol-5-il)uréia], BAP (6-benzilaminopurina) e ANA (ácido naftalenoacético). De modo geral, pôde-se observar resposta diferenciada dos clones quanto a intensidade, textura, coloração e grau de oxidação dos calos, em função dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento foram dos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1) e ANA (0,1 mg L-1), obtendo-se 100% de calejamento no explante caulinar. Houve a formação de estruturas nodulares compactas, principalmente na extremidade dos explantes caulinares, sendo essas regiões responsáveis pela regeneração de gemas adventícias. Em relação à regeneração, a melhor resposta foi obtida com 1,0 mg L-1 BAP.

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