Sources of acid insoluble ash as marker of corn digestibility in broilers

The objective was to evaluate three sources of acid insoluble ash (AIA) (celite, kaolin, and sand), as indicators, to determine the apparent metabolizable energy of corn on a natural matter (AME) basis, dry matter digestibility coefficient (DMDC), and crude protein digestibility coefficient (CPDC) using total and partial excreta collection methods. Two hundred and ten Ross broiler chicks of 18 to 27 days of age were used. Broilers were adapted to experimental the diet for four days, and excreta were collected for 5 days. A reference diet (RD) based on corn and soybean meal was prepared to meet the nutritional requirements of the birds. A test diet was prepared with 40% replacement of RD with corn. The formulated diets were: D1, 99% Reference diet + 1% celite; D2, 59.4% RD + 39.6 % corn + 1% celite; D3, 99% Reference diet + 1% kaolin; D4, 59.4% RD + 39.6 % corn + 1% kaolin; D5, 99% Reference diet + 1% sand; D6, 59.4% RD + 39.6 % corn + 1% sand. The treatments were distributed in a completely randomized design, with six diets and five replicates of seven birds each in a 2x3 factorial arrangement. There were significant interactions for all variables investigated. It was observed that the AME and DMDC values of corn were similar in the two methods of collection, and PDC values using kaolin and celite makers were similar. However, the AME and CPDC of corn using sand with partial collection method were underestimated by 17.70 and 15.53%, respectively compared to those with the total collection method. The AME values of corn with the collection methods using celite and, the DMDC using celite and sand were significantly different. It was observed that the DMDC using celite and sand provided significantly lower values (4.67 and 5.15%), respectively, and the AME using celite was 2.86% lower than that obtained by total collection. To determine the EMA, DMDC, and CPDC in broilers, it is more efficient to use celiteTM and kaolin as markers with partial collection of excreta. Sand should not be used with partial excreta collection method, because it provides lower values of AME and CPDC compared to with total excreta collection method.

The Effect of Intercropping of Lablab (Lablab purpureus L.) and Cowpea (Vigna unguiculata L.) at Different Planting Densities on in vitro and in sacco Dry Matter Digestibility of Napier Grass (Pennisetum purpureum)

Background: The advantage of intercropping is the more efficient utilization of the all available resources and the increased productivity compared with each sole crop of the mixture. If cowpea and Lablab intercropping with Napier grass its nutritional values was improved. Methods: The experimental design was factorial combination arrangement in randomized complete block design with three inter and intra spaces (1 m × 0.5 m, 0.75 m × 0.5 m, 0.5 m × 0.5 m) and intercropping with two tropical legumes. Treatments were T1= Pure Napier grass at 1 m row spacing, T2= Napier grass intercropped with lablab at 0.75 m row spacing, T3= Napier grass intercropped with cowpea at 0.5 m row spacing, T4= Napier grass intercropped with cowpea at 1 m row spacing, T5= Napier grass intercropped with lablab at 0.5 m row spacing, T6= Pure Napier grass at 0.75 m row spacing, T7= Napier grass intercropped with lablab at 1 m row spacing, T8= Napier grass intercropped with cowpea at 0.75 m row spacing, T9= Pure Napier grass at 0.5 m row spacing and totally nine treatments were used. Soil samples were collected before and after forage harvested. Result: Napier grass intercropped with lablab and cowpea at different planting densities had significant effect (P less than 0.05) on the in vitro dry and organic matter digestibility (IVDMD, IVOMD) and increased digestibility. The OM degradation constant was significantly different (P less than 0.05) but ‘ED’ was not and for DM degradation ‘c’ and ‘b’ were non-significant (P greater than 0.05) for Napier grass intercropped with lablab and cowpea at different planting densities. In conclusion, Napier grass intercropped with lablab and cowpea at a planting density of 24 plants m-2 was better choice for high yield and forage quality.

Effect of replacement of forage sorghum silage with biomass sorghum silage in diets for dairy heifers

This trial aimed to analyze the replacement of forage sorghum silage (FS silage) with biomass sorghum silage (BRS 716 silage) in diets for heifers ¾ Holstein x ¼ Zebu on their nutrient intake and digestibility, nitrogen balance, microbial production, feeding behavior, and animal performance. Eight heifers with average age 18±4 months (mo) and average body weight (BW) 271 ± 57 kg were used. The experimental design was double-Latin square, 4×4, being four diets, four experimental periods and four animals. Four experimental diets with 0, 33, 67 and 100% substitution of forage sorghum silage with biomass sorghum silage were used. The roughage:concentrate ratio in the total dry matter (DM) of the diets was 75:25. The fraction “b” of DM was higher in the BRS 716 silage. There was no difference (p> 0.05) for dry matter intake (DMI; p = 0.45) and total digestible nutrients intake (p = 0.76). The dry matter digestibility (DMD), crude protein, ether extract and neutral detergent fiber increased linearly with the inclusion of BRS 716 silage. The feeding times, ruminating and idle have not been changed (p > 0.05). The replacement of FS silage with BRS 716 silage did not modify the average daily weight gain (ADG; p > 0.05; mean 1.30 ± 0.025 kg). Biomass sorghum silage can replace up to 100% forage sorghum silage in the diets of ¾ Holstein x ¼ Zebu heifers.

In-vitro nutrient degradability of complete feed containing myristic acid and tannins addition

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility

Penggunaan Cairan Ekstrak Isi Gizzard dan Duodenum Ayam pada Pengukuran Kecernaan In Vitro Daun Turi (Sesbania grandiflora)

The aim of this study was to examine the use of Gizzard Contents Extracts (GCE) and Duodenum Contents Extracts (DCE) of Chicken on In Vitro digestibility measurements of Turi Leaves (Sesbania grandiflora). The study was designed using a One-way Classification of Completely Randomized Design (CRD) with 4 treatments. Each treatment was repeated three times. Types of treatment in the form of using GCE and DCE, including: (1) control treatments (without GCE or DCE), (2) GCE, (3) DCE, and (4) a mixture of GCE and DCE. The observed variables were Coefficient of Dry Matter Digestibility (CDMD), Coefficient of Organic Matter Digestibility (COMD), and Coefficient of Soluble Protein Digestibility (CSPD). The results showed that the addition of GCE, DCE, and a mixture of GCE and DCE could not increase the CDMD, COMD, and CSPD of Turi Leaves compared to the control treatment. Keywords: Duodenal contents extract; Gizzard content extract; In vitro digestibility; Turi leaves. Abstrak Penelitian bertujuan untuk mengkaji penggunaan cairan ekstrak isi gizzard (CEIZ) dan duodenum (CEID) ayam pada pengukuran kecernaan in vitro daun turi. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 4 perlakuan. Masing-masing perlakuan diulang tiga kali. Macam perlakuan berupa penggunaan penggunaan CEIZ dan CEID ayam, meliputi perlakuan kontrol (tanpa CEIZ maupun CEID), CEIZ, CEID, dan campuran CEIZ dan CEID. Variabel pengamatan berupa koefisien cerna bahan kering (KCBK), koefisien cerna bahan organic (KCBO), dan koefisien cerna protein terlarut (KCPT). Hasil penelitian menunjukkan bahwa perlakuan penambahan cairan ekstrak isi gizzard, duodenum dan campuran keduanya tidak dapat meningkatkan KCBK, KCBO, dan KCPT daun turi dibandingkan dengan perlakuan control. Kata kunci: Cairan ekstrak isi duodenum; Cairan ekstrak isi gizzard; Daun turi; Kecernaan in vitro.

Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

The accuracy of several in vitro methods in estimating in vivo digestibility of the tropical dairy ration

In vitro digestibility methods have been developed to overcome problems in the in vivo digestibility measurement, but its accuracy should be tested in a local setting. In vitro methods developed by Tilley and Terry (T2), Theodorou (T3) and Sutardi (T4) have been compared to in vivo method (T1) in a block randomized design study. Four heifers FH (337.50 ± 45.87 kg BW) were used in T1, and two fistulated FH bulls (510 ± 20 kg BW) were used as inoculant sources in the in vitro methods. Dairy cattle ration consisted of 54.0% Napier grass and 46.0% concentrate with 58.8% DM, 12.1% ash, 10.0% CP, 3.3% EE, 26.5% CF, and 61.1% TDN. The observed parameters were ration fermentability (pH, NH3, and VFA concentration) and digestibility (DMD and OMD). The data were analyzed using analysis of variance (ANOVA) followed by the Tukey test. The correlation was made before regression analysis to estimate the in vivo parameters from the in vitro. The results showed that pH values are in the normal range (6.7 – 6.8), and insignificantly different between treatments (P>0.05). The concentration of NH3 and VFA were significantly different between the treatments (P<0.05), but T2 produced similar NH3 and VFA concentrations to T1. Similar results were also found in the DMD and OMD. Correlation analysis showed that pH value of T3 correlated significantly with T1, while DMD value of T4 correlated to T1. The T1 DMD (Y) could be estimated from T4 DMD (X) using formula Y (%) = y = -0.091x2 + 9.1632x - 168.4. It is concluded that tropical dairy feedstuffs in vitro digestibility using Tilley and Terry’s method produced similar result to in vivo digestibility method, but in vivo dry matter digestibility can be estimated accurately by in vitro dry matter digestibility using Sutardi method.

DETERMINATION OF HERBAGE YIELD AND QUALITY OF DIFFERENT SOYBEAN VARIETIES

Objectives of the present study are to determine the variations in forage yield and quality of soybean cultivars. Experiments were conducted in randomized complete blocks design with 3 replications during the growing season of 2016. A total of 10 cultivars were used as the plant material of the experiments (Bravo, A3127, Traksoy, İlksoy, Mersoy, Nova, SA-88, Arısoy, Safir, Atakişi). Cultivars had a significant effect on yield and chemical composition. Results revealed that green herbage yields varied between 826.39 - 1199.17 kg/da, dry hay yields between 247.71 - 357.90 kg/da, crude protein yields between 16.91 - 39.86 kg/da, acid detergent fiber (ADF) ratios between 26.56 - 34.61%, neutral detergent fiber (NDF) ratios between 38.43 - 44.85%, crude ash contents between 7.20 - 11.22%, crude protein contents between 6.66 - 13.53%, dry matter digestibility (DDM) values between 61.94 - 68.21%, dry matter intake (DMI) values between 2.68 - 3.12% and relative feed values (RFV) between 2.68 - 3.12. The results of the study showed that A3127 cultivar was found to be prominent with green herbage yield (1199.17 kg/da) and dry hay yield (357.90 kg/da) and Safir cultivar was found to be prominent with crude protein yield (39.86 kg/da). It was concluded that the soybean cultivars A3127 and Safir could be recommended for hay production.

Evaluation of Digestability and Rumen Parameters Through In-vitro Concentrate Containing Binahong Flour as Secondary Compound

This study was conducted to determine the effect of different levels of binahong flour used in concentrate feed on dry matter digestibility (DMD), organic matter digestibility (OMD), VFA and NH3 concentrations in vitro. The design used was a completely randomized design with 4 treatments and 4 replications. The treatments studied were: R0; concentrate feed without binahong flour, R1; concentrate feed contains 10% binahong flour, R2; concentrate feed containing 20% binahong flour, R3; concentrate feed contains 30% binahong flour. Based on the results of the study, the average DMD (%) R0 73.56±0.75, R1 74.50 ±0.61, R2 74.98±0.70, R3 76.65±0.90, OMD (%) R0 69.50±1.10, R1 69.82±1.00, R2 70.07±0.88, R3 72.61±0.99, VFA concentration (mM) R0 108.53±8.52, R1 110.75±6.51, R2 111.40±2.35, R3 112.77 ±14.76, NH3 concentration (mM) R0 9.74±0.74, R1 10.61±0.44, R2 11.55±0.41, R3 11.55±0.33. The results of statistical analysis confirmed that the treatment had a very significant effect on the increase in DMD, OMD, NH3 (P<0,01), but not significant on the total VFA concentration. The conclusion of this study is that the use of binahong flour up to a level of 30% in concentrate feed has an effect on increasing in DMD, OMD, VFA, and NH3 concentrations in vitro.

Effects of Alysacrpus Monilifer fodder intake on feed consumption and performance of desert goats

This study was conducted to study the effects of the intake of the Alysacrpus Monilifera on dry matter intake, digestibility of nutrients and performance of Desert bucks. Twelve Desert goats bucks 4-5 years old with an average weight of 11 + 0.250 kg, were divided into three equal groups. The first group was fed A. Monilifera fodder, while the second group consumed the natural pasture free of A. Monilifera while the third group consumed the groundnut haulms. After observing the cases of deadly diarrhea in the first group, the feeding pattern was modified with 50% natural pasture and 50% A. Monilifer fodder. The fodder was analyzed as well as conducting in vitro digestibility. The experimental design was randomized and the data were analyzed using the analysis of variance and the detection of differences among means using least significant difference test. The results showed that feeding A. Monilifer alone resulted in fatal diarrhea in bucks without affecting appetite, and the dry matter digestibility and organic matter in vitro digestibility was significantly (P<0.05) higher in A. Monilifer than compared to the groundnut haulms or the natural grazing. The weight gain was significantly (P<0.05) higher for the group on groundnut haulms and the natural grazing than those on A. Monilifer alone but when 50% of the plant biomass was replaced by the natural grazing the gain was significantly higher. The study concluded that the intake of A. Monilifer at the growth stage was good quality roughage when fed as part of the diet.