scholarly journals Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

2017 ◽  
Vol 5 (2) ◽  
pp. 15-26 ◽  
Author(s):  
Raihan I Raju ◽  
Shyamal K Roy
Keyword(s):  
In Vitro Culture ◽  
Basal Medium ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Mass Propagation ◽  
Surface Sterilization ◽  
Half Strength

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog’s (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.Jahangirnagar University J. Biol. Sci. 5(2): 15-26, 2016 (December)

scholarly journals Establishment of in vitro regeneration protocol for Adhatoda vasica Nees

2016 ◽  
Vol 51 (1) ◽  
pp. 75-80 ◽  
Author(s):  
S Khan ◽  
S Akter ◽  
A Habib ◽  
TA Banu ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Adhatoda Vasica

An in vitro regeneration protocol of Adhatoda vasica has been developed using excised nodal segments and juvenile leaves for multiple shoots regeneration directly or through callus induction. Explants were cultured on MS medium with different concentrations of IAA, NAA, BAP, GA3 and Kn singly or in combinations. MS medium supplemented with BAP (10.0 mg/l) was found best for multiple shoot formation, in which 93.33% explants produced multiple shoots. After two months, maximum number of multiple shoots were 10.6 ± 1.82, highest length of plantlets was 5.2 ± 2.20 cm. 100% calli formation were observed on MS medium supplemented with IAA (0.05 mg/l) + NAA (0.05 mg/l) + BAP (1.0 mg/l). Callus initiation started after 14 days and gave light green colored callus. Best callus mediated shoot regeneration was found on MS+10.0 mg/l BAP medium. Root induction of in vitro raised shoots was best on ½ MS + IBA (1.0 mg/l). Well rooted plantlets were transferred to plastic pots containing garden soil and compost in a ratio of 2:1 for hardening. The ultimate survival rate under natural condition was about 80%.Bangladesh J. Sci. Ind. Res. 51(1), 75-80, 2016

scholarly journals In vitro regeneration for mass propagation in commercial scale of medicinal plant vitex nigundo L.

1970 ◽  
Vol 18 ◽  
pp. 140-145 ◽  
Author(s):  
Md Abu Hena Mostofa Jamal ◽  
ANM Rubaiyath-Bin Rahman ◽  
Dipak Kumar Paul ◽  
Md Rezuanul Islam
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Commercial Scale

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferation and root induction were recorded after three weeks of inoculation and used for calculation. For each treatment 15 explants were used and all the treatments were repeated thrice. Established plantlets were transplanted in earthen pots under natural conditions and the survival rate was recorded. Results: The most effective surface sterilization treatment has been found 0.1 % HgCl2 for 7 minutes. Highest number of shoot was observed in MS medium supplemented with 3.0 mg/ BAP. It was rooted well in full MS containing 2.0 mg/l IAA. The survival rate was 85 % and propagated plantlets were successfully acclimatized in soil. Conclusion: It was observed that shoot tips are more responsive for micropropagation of Vitex nigundo L . Thus the fruitful utilization of rapid clonal propagation, germplasm conservation and distribution of Vitex nigundo, important medicinal plant of Bangladesh, is possible. Keywords: Vitex nigundo; Medicinal plant; Shoot induction; Micropropagation; Regeneration. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8790 JBS 2010; 18(0): 140-145

scholarly journals Micropropagation of an important medicinal herb Eclipta alba (L.) Hassk.

2016 ◽  
Vol 4 (1) ◽  
pp. 61-69 ◽  
Author(s):  
S Yesmin ◽  
A Hashem ◽  
MS Islam
Keyword(s):  
Morphological Variation ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Induction Medium ◽  
Root Induction ◽  
Ms Medium ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

Nodal segments from naturally grown Eclipta alba (L.) Hassk.were used as explants for organogenesis. Multiple shoots were obtained from the explants cultured on MS medium supplemented with various concentrations of BAP and Kn alone or in combination with NAA and IAA. Maximum number of multiple shoots (18.40±0.67) were induced on MS medium supplemented with 1.0 mg/l BAP and 0.1mg/NAA. In vitro raised shoots were cultured onto half and full strength MS medium supplemented with different concentration of IBA, IAA and NAA. The best root induction medium was found to be half strength MS containing 0.1 mg/l IBA where 96% shoots rooted. Regenerated plantlets grew normally without showing any morphological variation and flowered after 45 days of transplantation.Jahangirnagar University J. Biol. Sci. 4(1): 61-69, 2015 (June)

scholarly journals In Vitro Regeneration and Mass Propagation of Ruta graveolens L.—A Multipurpose Shrub

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1478-1480 ◽  
Author(s):  
Mohd Faisal ◽  
Naseem Ahmad ◽  
Mohammad Anis
Keyword(s):  
High Frequency ◽  
In Vitro Regeneration ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ruta Graveolens ◽  
Mass Propagation ◽  
Regeneration Frequency ◽  
Whole Plant ◽  
Half Strength

A protocol for rapid in vitro propagation of Ruta graveolens L. through high-frequency shoot induction from nodal explants was established. Proliferation of shoots from nodal segments was achieved on Murashige and Skoog medium supplemented with various concentrations of BA, Kin, IAA, and NAA, either singly or in various combinations. The highest shoot regeneration frequency (98.5%) and the highest number of shoots per explant (40.2 ± 2.8) was obtained on MS medium supplemented with 10 μm BA and 2.5 μm NAA. In vitro regenerated shoots rooted best on half-strength MS medium containing 0.5 μm IBA. Rooted shoots, following acclimatization in the greenhouse, were successfully transferred to field conditions, and 90% of plants survived. The efficient in vitro regeneration of the whole plant can be used as a fast and reliable method to transform R. graveolens genetically for its active principles.

scholarly journals Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

HortScience ◽  
2020 ◽  
Vol 55 (4) ◽  
pp. 424-428 ◽  
Author(s):  
Alvine Ornella Tchouga ◽  
Vincent Deblauwe ◽  
Stephanie Astride Mouafi Djabou ◽  
Giovanni Forgione ◽  
Rachid Hanna ◽  
...  
Keyword(s):  
Survival Rate ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Tap Root ◽  
Conservation Effort ◽  
Long Time ◽  
Half Strength ◽  
Time Required

The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts.

scholarly journals In vitro multiplication, micromorphological studies and ex vitro rooting of Hybanthus enneaspermus (L.) F. Muell. – a rare medicinal plant

2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Nodal Segments ◽  
Ms Medium ◽  
Ex Vitro ◽  
Culture Bottle ◽  
Hybanthus Enneaspermus ◽  
Half Strength

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.

scholarly journals Research on in vitro micropropagation of Lilium brownii F.E. Brown

2016 ◽  
Vol 14 (1) ◽  
pp. 121-129
Author(s):  
Vũ Hoài Sâm ◽  
Bùi Đức Quỳnh ◽  
Nguyễn Thị Hương ◽  
Nguyễn Văn Khiêm
Keyword(s):  
Subtropical Climate ◽  
Ms Medium ◽  
In Vitro Plant Regeneration ◽  
Medicinal Species ◽  
Surface Sterilization ◽  
The North ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Over Exploitation

Lilium brownii Brown belonging Lilium genus and Liliaceae family is well-known as a popular medicinal species, as well as food source and beautiful ornamental flowers. The specie has unique and ornamental floral characteristics such as light and elegant fragrance and perianth color rapidly changing from yellowish cream to white during anthesis. In traditional medicine, it is used for treatment cough, sedation diuretic, bronchitis... In nature, it can be found in subtropical climate moutainous areas in the North such as Sa Pa, Bat Xat, Mu Cang Chai; Sin Ho and Phong Tho, Quang Ba and Dong Van. In recent years, this species has been listed in the Red List for medicinal plants in Vietnam due to over-exploitation. The only effective strategy for sustaible conservation this species is in vitro micropropagation. In this study, in vitro plant regeneration and micropropagation of L. brownii was established from bubles and stem nodes. After surface sterilization with 0.1% HgCl­2 in 10 minutes, healthy young shoots were obtained from initial bubles and stem nodes on MS medium supplemented with 0.5 mg/l BAP or 0.5 mg/l NAA, respectively.  Bulblets also were formed from young shoot on MS supplemented with 0.5 mg/l NAA. The highest number of 4.5 bulblets per an explant was recorded from longitude-divided bubbles on MS medium containing 0.5 mg/l NAA and 0.2 mg/l BAP after 60 days in culture. The regererated plants produced quality roots on half strength MS supplemented with the combination of 1.0 mg/ l NAA and 0.2 mg / l BAP. More than 90% of rooted plants in vitro were survival on artificial soil TN1 in the nursery.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

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