Effects of gibberellin GA7 on kinetics of growth and tropane alkaloid accumulation in hairy roots of Brugmansia candida

1997 ◽  
Vol 33 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Sandra Irene Pitta-Alvarez ◽  
Ana María Giulietti
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid ◽  
Brugmansia Candida ◽  
Kinetics Of

Biotechnological Interventions of Hairy Roots of Tropane Alkaloid-Bearing Plants

Hairy Roots ◽  
2018 ◽  
pp. 71-93
Author(s):  
Guoyin Kai ◽  
Weiwei Zhao ◽  
Min Shi ◽  
Yao Wang
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid

Tropane Alkaloid Production by the Establishment of Hairy Root Cultures of Brugmansia candida and Elicitation

2020 ◽  
pp. 123-132
Author(s):  
Alejandra B. Cardillo ◽  
María Perassolo ◽  
Juan M. Minoia ◽  
Julián Rodríguez Talou ◽  
Ana M. Giulietti
Keyword(s):  
Hairy Root ◽  
Tropane Alkaloid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Brugmansia Candida

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Occurrence of cadaverine in hairy roots of Brugmansia candida

2001 ◽  
Vol 57 (5) ◽  
pp. 759-763 ◽  
Author(s):  
Carla N. Carrizo ◽  
Sandra I. Pitta-Alvarez ◽  
Marcelo J. Kogan ◽  
Ana M. Giulietti ◽  
Marı́a L. Tomaro
Keyword(s):  
Hairy Roots ◽  
Brugmansia Candida

Tropane alkaloid patterns in plants and hairy roots of Hyoscyamus albus

1993 ◽  
Vol 35 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Katja Doerk-Schmitz ◽  
Ludger Witte ◽  
A.Wilhelm Alfermann
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid ◽  
Hyoscyamus Albus

Hairy Roots of Brugmansia candida That Grow without Agitation: Biotechnological Implications

2001 ◽  
Vol 17 (4) ◽  
pp. 661-663
Author(s):  
S.I. Pitta-Alvarez ◽  
A.M. Giulietti
Keyword(s):  
Hairy Roots ◽  
Brugmansia Candida

Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.

2017 ◽  
Vol 54 (1) ◽  
pp. 117-126 ◽  
Author(s):  
A. Moussous ◽  
C. Paris ◽  
M. Khelifi-Slaoui ◽  
M. Bekhouche ◽  
D. Zaoui ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Root Biomass ◽  
Tropane Alkaloid ◽  
Pseudomonas Spp ◽  
Transgenic Hairy Roots
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