Biotechnological Interventions of Hairy Roots of Tropane Alkaloid-Bearing Plants

Hairy Roots ◽  
2018 ◽  
pp. 71-93
Author(s):  
Guoyin Kai ◽  
Weiwei Zhao ◽  
Min Shi ◽  
Yao Wang
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid

CRISPR/Cas9-mediated disruption of the PYRROLIDINE KETIDE SYNTHASE gene reduces the accumulation of tropane alkaloids in Atropa belladonna hairy roots

2021 ◽  
Author(s):  
Fumihito Hasebe ◽  
Honoka Yuba ◽  
Takashi Hashimoto ◽  
Kazuki Saito ◽  
Nobutaka Funa ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Biosynthetic Pathway ◽  
Polyketide Synthase ◽  
Tropane Alkaloids ◽  
Tropane Alkaloid ◽  
Atropa Belladonna ◽  
Type Iii Polyketide Synthase ◽  
Canonical Type ◽  
Solanaceae Family

Abstract Tropane alkaloids, including clinically important hyoscyamine and scopolamine, are produced in the roots of medicinal plant species, such as Atropa belladonna, from the Solanaceae family. Recent molecular and genomic approaches have advanced our understanding of the metabolic enzymes involved in tropane alkaloid biosynthesis. A non-canonical type III polyketide synthase (PKS), pyrrolidine ketide synthase (PYKS), catalyzes a two-step decarboxylative reaction, which involves imine-ketide condensation indispensable to tropane skeleton construction. In this study, we generated pyks mutant A. belladonna hairy roots via CRISPR/Cas9-mediated genome editing and analyzed the metabolic consequences of the loss of PYKS activity on tropane alkaloids, providing insights into a crucial role of the scaffold-forming reaction in the biosynthetic pathway.

Tropane alkaloid patterns in plants and hairy roots of Hyoscyamus albus

1993 ◽  
Vol 35 (1) ◽  
pp. 107-110 ◽  
Author(s):  
Katja Doerk-Schmitz ◽  
Ludger Witte ◽  
A.Wilhelm Alfermann
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid ◽  
Hyoscyamus Albus

Tailoring Tropane Alkaloid Accumulation in Transgenic Hairy Roots of Atropa baetica by Over-expressing the Gene Encoding Hyoscyamine 6β-hydroxylase

2006 ◽  
Vol 28 (16) ◽  
pp. 1271-1277 ◽  
Author(s):  
Rafael Zárate ◽  
Nabil el Jaber-Vazdekis ◽  
Braulio Medina ◽  
Ángel G. Ravelo
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid ◽  
Gene Encoding ◽  
Transgenic Hairy Roots ◽  
Atropa Baetica

Pseudomonas spp. increases root biomass and tropane alkaloid yields in transgenic hairy roots of Datura spp.

2017 ◽  
Vol 54 (1) ◽  
pp. 117-126 ◽  
Author(s):  
A. Moussous ◽  
C. Paris ◽  
M. Khelifi-Slaoui ◽  
M. Bekhouche ◽  
D. Zaoui ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Root Biomass ◽  
Tropane Alkaloid ◽  
Pseudomonas Spp ◽  
Transgenic Hairy Roots

Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production

2006 ◽  
Vol 39 (6) ◽  
pp. 1183-1189 ◽  
Author(s):  
Laiq ur Rahman ◽  
Yoshie Kitamura ◽  
Junko Yamaguchi ◽  
Miki Mukai ◽  
Kuniko Akiyama ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Tropane Alkaloid ◽  
Alkaloid Production
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