Recently, iron (Fe) is introduced to substitute vanadium (V) in Ti-alloy. Therefore, new (α+β) titanium alloy, Ti-6Al-1Fe was designed through a complete replacement of V by Fe with major composition modifications of Ti-6Al-4V. This new alloy is believed could provide similar properties of Ti-6Al-4V through modification of its microstructures. Different heat treatments can lead to a diversity of microstructural permutations and combinations. Thus, it is very crucial to study in-depth understanding about the microstructure of Ti-6Al-1Fe. Results reveal that the microstructure of as-received alloy is a typical fine lamellar microstructure. The bi-modal microstructure can be obtained by hot rolling below beta-transus temperature (Tβ) followed by recrystallization treatment at 925°C. While cold rolling followed by recrystallization treatment at 925°C produce equiaxed microstructure.