High frequency in vitro propagation of Trichopus zeylanicus subsp. travancoricus using branch–petiole explants

2010 ◽  
Vol 33 (4) ◽  
pp. 1141-1148 ◽  
Author(s):  
Kottackal Poulose Martin ◽  
A. K. Pradeep ◽  
Joseph Madassery
Keyword(s):  
High Frequency ◽  
In Vitro Propagation ◽  
Petiole Explants

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds

1998 ◽  
Vol 18 (3-4) ◽  
pp. 336-340 ◽  
Author(s):  
E. Wildi ◽  
W. Schaffner ◽  
K. Berger Büter
Keyword(s):  
In Vitro Propagation ◽  
Petiole Explants

High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara

2009 ◽  
Vol 32 (2) ◽  
pp. 405-409 ◽  
Author(s):  
R. Thirugnanasampandan ◽  
G. Mahendran ◽  
V. Narmatha Bai
Keyword(s):  
High Frequency ◽  
In Vitro Propagation

scholarly journals In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

2009 ◽  
Vol 18 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H. David Raja ◽  
D.I. Arockiasamy
Keyword(s):  
High Frequency ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Root Induction ◽  
Root Proliferation ◽  
Key Words Micropropagation ◽  
Shoot Tip Explants ◽  
Important Medicinal Plant

A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 - 4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 - 2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 - 95% after 25 days. Key words: Micropropagation, Mentha viridis, Node, Shoot tip, Multiple shoots D.O.I. 10.3329/ptcb.v18i1.3243 Plant Tissue Cult. & Biotech. 18(1): 1-6, 2008 (June)

scholarly journals In-vitro Conservation of Bacopa monniera (L.) Wettst.: A Memory Booster Plant

Our Nature ◽  
1970 ◽  
Vol 8 (1) ◽  
pp. 40-47 ◽  
Author(s):  
J. Prabha ◽  
U. Rani ◽  
A. Sen ◽  
R.N. Verma ◽  
A. Batra
Keyword(s):  
Growth Regulators ◽  
High Frequency ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Segment ◽  
Bud Break ◽  
Ms Medium ◽  
Bacopa Monniera ◽  
In Vitro Shoot Regeneration

A mass in-vitro propagation system of Bacopa monniera (L.) Wettst., a traditional medicinal herb, has been developed. Shoot proliferation and growth were greatly influenced by the months of the year during which the explant were collected. High frequency bud break coupled with maximum number of shoots through nodal segment were found in the month of June and August. Of the different growth regulators tried, for in vitro shoot regeneration, MS medium with BAP (1.0 mg/l) induced maximum number of proliferative shoots (65.00±1.453). Multiplication and elongation of the shoots were obtained after regular sub culturing on the same medium after 15-21 days. Rooting from basal end of the shoots occurred on MS medium with the addition of IBA (0.5 mg/l). After a hardening phase of 4 weeks, almost 95% transplantation was success in the field.DOI: 10.3126/on.v8i1.4311

scholarly journals AN EFFICIENT IN VITRO REGENERATION METHOD TO PRODUCE ADVENTITIOUS PLANTS IN SWEETPOTATO

HortScience ◽  
1993 ◽  
Vol 28 (4) ◽  
pp. 262B-262
Author(s):  
C. S. Prakash ◽  
R. Gosukonda ◽  
A. Porobo Dessai ◽  
E. Blay ◽  
K. Dumenvo
Keyword(s):  
Gene Transfer ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Zeatin Riboside ◽  
Limiting Factor ◽  
Ms Medium ◽  
Petiole Explants ◽  
Green House

Lack of suitable methods to develop adventitious plantlets in vitro is a limiting factor in producing transgenic sweetpotato plants through gene transfer. Studies were conducted to develop an in vitro high frequency regeneration protocol for sweetpotato that is rapid and consistent. When 27 genotypes of sweetpotato were screened, five were identified as highly regenerative (318846-3, PI 531143, Hi Dry, Rojoblanco and Beauregard). High frequency regeneration of shoots (in 60 to 80% explants) was observed within 30 days when leaf explants with intact petioles from the apical portions of the in vitro shoots were cultured on a MS medium with 2,4-D (0.2 mg/l) for three days and then transferred to a medium with zeatin riboside (ZR) (0.2 mg/l). However, thidiazuron (0.2 mg/l) had to be substituted for ZR to achieve regeneration of shoots from petiole (0.5 to 1 cm) explants (the most responsive organ for transformation by Agrobacterium). Petiole explants developed shoots efficiently (80-90%) and rapidly (10 to 21 d), but were specific to the genotype 318846-3. The resulting plantlets were vigorous and normal, and were transferred to the green house with little or no mortality.

Sign in / Sign up

Export Citation Format

Share Document