Effect of culture container and carbohydrate content on in vitro slow growth storage of the cherry rootstock ‘Gisela®5’

2017 ◽  
Vol 39 (4) ◽  
Author(s):  
Elif Aylin Ozudogru ◽  
Carla Benelli ◽  
Giuliano Dradi ◽  
Maurizio Lambardi
2021 ◽  
Author(s):  
Paulo Hercilio Viegas Rodrigues ◽  
Emerson Oliveira ◽  
Christian Demetrio ◽  
Guilherme Ambrosano ◽  
Sônia Maria Stefano Piedade

Abstract Maintaining updated in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delay and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra did not survive after 140 days of in vitro slow-growth storage. The plantlets maintained under the B spectrum survived after 140 days of in vitro slow-growth storage and showed little browning.


2019 ◽  
pp. 397-416 ◽  
Author(s):  
Ravishankar Chauhan ◽  
Vikram Singh ◽  
Afaque Quraishi

2017 ◽  
pp. 101-106 ◽  
Author(s):  
A. Arbeloa ◽  
J.A. Marín ◽  
P. Andreu ◽  
E. García ◽  
P. Lorente

2012 ◽  
pp. 89-93 ◽  
Author(s):  
C. Benelli ◽  
E.A. Ozudogru ◽  
M. Lambardi ◽  
G. Dradi

2006 ◽  
pp. 483-488 ◽  
Author(s):  
M. Lambardi ◽  
R. Roncasaglia ◽  
A. Previati ◽  
A. De Carlo ◽  
G. Dradi ◽  
...  

2015 ◽  
Vol 37 (11) ◽  
Author(s):  
Alina Trejgell ◽  
Monika Kamińska ◽  
Andrzej Tretyn

2017 ◽  
pp. 37-44 ◽  
Author(s):  
F. Gomes ◽  
M. Clemente ◽  
P. Figueiredo ◽  
F. Plácito ◽  
H. Machado ◽  
...  

2021 ◽  
Vol 13 (12) ◽  
pp. 6743
Author(s):  
Veerala Priyanka ◽  
Rahul Kumar ◽  
Inderpreet Dhaliwal ◽  
Prashant Kaushik

Germplasm is a valuable natural resource that provides knowledge about the genetic composition of a species and is crucial for conserving plant diversity. Germplasm protection strategies not only involve rescuing plant species threatened with extinction, but also help preserve all essential plants, on which rests the survival of all organisms. The successful use of genetic resources necessitates their diligent collection, storage, analysis, documentation, and exchange. Slow growth cultures, cryopreservation, pollen and DNA banks, botanical gardens, genetic reserves, and farmers’ fields are a few germplasm conservation techniques being employed. However, the adoption of in-vitro techniques with any chance of genetic instability could lead to the destruction of the entire substance, but the improved understanding of basic regeneration biology would, in turn, undoubtedly increase the capacity to regenerate new plants, thus expanding selection possibilities. Germplasm conservation seeks to conserve endangered and vulnerable plant species worldwide for future proliferation and development; it is also the bedrock of agricultural production.


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