PENGARUH WARNA WADAH BERBEDA TERHADAP PERTUMBUHAN NANNOCHLOROPSIS SP

Pertumbuhan fitoplankton dipengaruhi oleh beberapa faktor fisika seperti suhu, cahaya matahari, kedalaman, kekeruhan, salinitas, dan kandungan oksigen, faktor kimia seperti pH, fosfat, nitrat, nitrit, dan silikat serta warna wadah kultur. Tujuan penelitian untuk mengetahui pengaruh warna wadah terhadap pertumbuhan Nanochloropsis sp. Penelitian didesain menggunakan Rencana Acak Lengkap (RAL). Perlakuan yang diuji adalah penggunaan wadah kultur yang memiliki warna berbeda dengan empat perlakuan dan tiga kali ulangan. Warna wadah yang digunakan adalah merah, kuning, biru dan bening. Hasil penelitian menunjukkan penggunaan warna wadah berpengaruh nyata terhadap pertumbuhan Nannochloropsis sp. Warna wadah terbaik untuk pertumbuhan Nannochloropsis sp. adalah bening. Phytoplankton growth is influenced by several physical factors such as temperature, sunlight, depth, turbidity, salinity, and oxygen content, chemical factors such as pH, phosphate, nitrate, nitrite, and silicate as well as the color of the culture container. The purpose of the study was to determine the effect of container color on the growth of Nanochloropsis sp. The study was designed using a Completely Randomized Plan (CRD). The treatment tested was the use of culture containers that had different colors with four treatments and three replications. The color of the container used is red, yellow, blue and clear. The results showed that the use of container color significantly affected the growth of Nannochloropsis sp. The best container color for the growth of Nannochloropsis sp. is clear.

Growth Response of Porphyridium sp. Culture on Glass and Plastic Container in Laboratory Scale

Utilization of Porphyridium sp. began to be developed as an anticancer, anti-inflammatory, antibacterial, antifungal, and production of biogas and biodiesel so that necessary to carry out culture activities for ensure continuity of Porphyridium sp. This study aims to determine the type of culture container that showed the best growth response of Porphyridium sp. with the highest biomass. The stages in this research were preparation of containers and media sterilization, making diatom fertilizers and agar media, Culture of Porphyridium sp. on agar media, Culture of Porphyridium sp. in the test tube, and Culture of Porphyridium sp. in glass and plastic containers. The results showed that the growth response of Porphyridium sp. that cultured in glass containers (18,9 ± 0,21 x 105 cells/mL) was higher than plastic (15.57 ± 0,03 x 105 cells/ml).

Effect of Container Culture Types on The Success of Cacao Somatic Embryos Formation

<p><em>Container culture have an important role in determining the success of in vitro culture since it will affect the development of culture, such as the formation of embryonic structures. The study aimed to determine the effect of culture container types on cacao somatic embryogenesis. The study was conducted at Tissue Culture Laboratory, Superior Seed Development Unit of IAARD, Bogor, from April to September 2016. The tests were conducted on the effect of container and explant types as well as the effect of container types and genotypes. The effects of container and explant types were tested using callus induced from petal and staminoid explants of Sca 6, whereas the effects of container types and genotypes were tested using callus induced from petal explants of Sca 6 and ICCRI 4. Afterwards, the somatic embryos were induced using petri dishes or culture bottles according to treatment. The results showed no significant interaction between container and explant types on the average percentage of the formation and number of somatic embryos (10.28% embryos/explants in culture bottles and 7.89% embryos/explants in petri dishes). Meanwhile, there was significant interaction between genotypes and container types in the initial period of somatic embryos formation (15 and 18 weeks after culture), but the effect was not significant in the final period of observation (21 weeks after culture). The results indicate that culture bottles, which have lower prices, can be used to replace petri dishes to induce the formation of somatic embryos in cacao.</em></p>

UTILIZATION OF TOFU AND TAPIOCA INDUSTRIAL LIQUID WASTE FOR NILE TILAPIA (Oreochromis niloticus) CULTURE WITHIN DIFFERENT BIOFLOC SYSTEMS

Liquid waste produced by tofu and tapioca industry was approximately 1,5-2 m3 and 4-6 m3 per day respectively. Tapioca liquid waste has concentration of carbon around 119,11 mg/l, while tofu liquid waste has around 133,03 mg/l of nitrogen in concentration. Therefore both of these waste have the potential to be used as biofloc that utilized as additional feed with high protein content for nile tilapia. The aim of this research was to know interaction between C/N ratio and place of biofloc production to the growth of nile tilapia. This research used completely randomized design based on factorial experiment which consisted of two level of each factor and three repetition. Level of C/N ratio were 15 and 20 whereas level of place of biofloc production were inside and outside fish culture container. The result of this research showed that interaction between place of biofloc production and C/N ratio affected the growth of nile tilapia. The treatment inside biofloc production with 20 C/N ratio gave provided the highest absolute growth (3,26 g) and daily growth rate (0,082 g per day).

Budidaya Maggot (Hermetia illuens) dengan menggunakan beberapa media

The purpose of this study was to determine the best culture medium for producing maggot (Hermetia illucens) and to determine the production quantity in each different medium. This research was controlled two times a day at 09:00 a.m. and 16:00 p.m. Cultivation media were prepared from coconut pulp, tofu pulp, rice bran and restaurant wastes. Each medium was weighed as much as one kg and then placed into a culture container. The research used Completely Randomized Design consisting of 4 treatments, each with three replications. The parameters observed consisted of temperature and pH of the media measured at 07.00 am and 17.00 pm, and maggot production measured on the last day. During the ten days of experiment, the production of maggot in the treatment using restaurant waste media was significantly different as compared to other treatments. This study found that a good medium for the production of maggot was prepared using restaurant waste. Keywords: Hermetia illucens, coconut pulp, restaurant waste

MANAJEMEN KULTUR ROTIFER DENGAN TANGKI VOLUME KECIL

Keberhasilan pembenihan ikan sangat dipengaruhi keberhasilan produksi jasad pakan rotifer secara tepat dan efisien. Penelitian kultur rotifer dengan tangki volume kecil bertujuan untuk mendapatkan efisiensi produksi yang paling optimal dan memenuhi prinsip dasar akuakultur low volume high density. Penelitian menggunakan tangki polyethylene dengan volume 500 L dan volume media awal 100 L, padat tebar awal 200 ind. rotifer per mL dengan sediaan pakan dasar fitoplankton Nannocloropsis occulata, ragi roti (0,05 g/mio.rot./feeding) dan suplemen Scott emulsion (0,005 g/mio.rot./feeding). Penelitian dilakukan secara bertahap; tahap pertama (I) tanpa penambahan air laut, peningkatan volume hanya dari penambahan 15 L Nannochloropsis tiap hari sampai hari kelima, tahap kedua (II) dengan penambahan alga 40 L dan air laut 40 L; serta tahap ketiga (III) dengan menggandakan pemberian ragi roti. Hasil penelitian menunjukkan bahwa pada percobaan tahap I: total produksi rata-rata 122,37 x 106 ind. rotifer, pada tahap II: 97,67 x 106 ind. rotifer, dan pada tahap III: dicapai rata-rata total produksi tertinggi dengan 187,17 x 106 ind. rotifer per tanki kultur 500 L. Pengelolaan kultur pada tahap III memberikan hasil terbaik dengan simpangan terkecil antar tangki kultur ulangan, dan membuktikan sebagai pengelolaan terbaik untuk kultur rotifer dengan tangki volume kecil. Success of marine seed production is highly influenced by effective and efficient production performance of life food rotifer. Observation on rotifer culture using small volume tank was aimed to get the optimum production and efficiency, to fulfill the basic principle of aquaculture “low volume high density”. Polyethylene tanks of 500 L. were used as culture container, with initial 100 liter sea water as culture medium and initial density of 200 ind. rotifer per mL. N. occulata, baker yeast (0.05 g/mio.rotifer/feeding) and Scott emulsion (0.005 g/mio.rotifer/feeding) were used as basic feed, and applied differently among three trials. First trial without seawater addition, increasing volume of culture media was only from 15 L. of N. occulata within 5 days culture, second trial was done with addition of seawater of 40 L and 40 L of N. occulata every day; and the last trial with twice dosage of baker yeast from trial I and II. The result showed that the average total production from the first trial was 122.37 x 106 ind. rotifer and the second trial was decreased to 97.67 x 106 ind. rotifer. Highest average total production was achieved by the last trial with 187.17 x 106 ind. rotifer per culture tank 500 L. Culture management on the third trial gave the best result with the lowest deviation among replication tanks, and proved as the best management practice for small-scale culture container.

The tubulogenic effect of aldosterone is attributed to intact binding and intracellular response of the mineralocorticoid receptor

Little is known about the extra- and intracellular stimuli inducing renal stem/progenitor cells to develop into three-dimensionally structured tubules. To study this specific development in a controlled environment, we used an advanced culture technique. Embryonic tissue derived from neonatal rabbit kidney was placed in a perfusion culture container at the interface of an artificial interstitium made of a polyester fleece. Culture was carried out in chemically defined Iscove’s Modified Dulbecco’s Medium (IMDM) for 13 days. Development of tubules was histochemically detected on cryosections labeled with Soybean Agglutinin (SBA). The experiments showed that aldosterone exerts a specific tubulogenic effect. Application of aldosterone (1 × 10−7 M) raised numerous SBA-labeled tubules, while in the absence of the steroid hormone the development of tubules was lacking. Specificity of hormone action was analyzed by the use of aldosterone antagonists. Administration of spironolactone (1 × 10−4 M) and canrenoate (1 × 10−5 M) completely inhibited the development of tubules. Finally, disrupting the intracellular molecular complex of the mineralocorticoid receptor (MR) and heat shock proteins by geldanamycin (2 μg/ml) prevented the development of tubules. Our results suggest that the tubulogenic effect induced by aldosterone is attributed to both hormone binding and an undisturbed intracellular response of the MR.