Acquisition of seed dormancy breaking in rice (Oryza sativa L.) via CRISPR/Cas9-targeted mutagenesis of OsVP1 gene

2019 ◽  
Vol 13 (5) ◽  
pp. 511-520 ◽  
Author(s):  
Yu Jin Jung ◽  
Hyo Ju Lee ◽  
Sangsu Bae ◽  
Jong Hee Kim ◽  
Dong Hyen Kim ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L ◽  
Targeted Mutagenesis ◽  
Dormancy Breaking

scholarly journals Genetic Dissection of Seed Dormancy in Rice (Oryza sativa L.) by Using Two Mapping Populations Derived from Common Parents

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Chaopu Zhang ◽  
Zhiyang Yuan ◽  
Yuntong Wang ◽  
Wenqiang Sun ◽  
Xinxin Tang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L ◽  
Genetic Dissection ◽  
Mapping Populations

Identification of Marker Loci for Seed Dormancy in Rice (Oryza sativa L.)

Crop Science ◽  
1997 ◽  
Vol 37 (6) ◽  
pp. 1759-1763 ◽  
Author(s):  
J. Wan ◽  
T. Nakazaki ◽  
K. Kawaura ◽  
H. Ikehashi
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L ◽  
Marker Loci

Transcriptomics Analysis Identified Candidate Genes Colocalized with Seed Dormancy QTLs in Rice (Oryza sativa L.)

2010 ◽  
Vol 53 (5) ◽  
pp. 330-337 ◽  
Author(s):  
Huaide Qin ◽  
Fuqing Wu ◽  
Kun Xie ◽  
Zhijun Cheng ◽  
Xiuping Guo ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Candidate Genes ◽  
Seed Dormancy ◽  
Oryza Sativa L

Identification of QTLs for seed dormancy in rice (Oryza sativa L.)

2010 ◽  
Vol 130 (3) ◽  
pp. 328-332 ◽  
Author(s):  
Kun Xie ◽  
Ling Jiang ◽  
BingYue Lu ◽  
ChunYan Yang ◽  
LinFang Li ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L

scholarly journals OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)

2020 ◽  
Vol 21 (11) ◽  
pp. 4023
Author(s):  
Qingfei Wu ◽  
Cheng Zhang ◽  
Yue Chen ◽  
Kaiyue Zhou ◽  
Yihua Zhan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Oryza Sativa ◽  
Chloroplast Development ◽  
Oryza Sativa L ◽  
Rice Genome ◽  
Targeted Mutagenesis ◽  
Label Free ◽  
Microscopy Observation ◽  
Leaf Phenotype ◽  
Chaperonin 60

The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types—Cpn60α and Cpn60β and the rice genome encodes three α and three β plastid chaperonin subunits. However, the functions of Cpn60 family members in rice were poorly understood. In order to investigate the molecular mechanism of OsCpn60β1, we attempted to disrupt the OsCpn60β1 gene by CRISPR/Cas9-mediated targeted mutagenesis in this study. We succeeded in the production of homozygous OsCpn60β1 knockout rice plants. The OsCpn60β1 mutant displayed a striking albino leaf phenotype and was seedling lethal. Electron microscopy observation demonstrated that chloroplasts were severely disrupted in the OsCpn60β1 mutant. In addition, OsCpn60β1 was located in the chloroplast and OsCpn60β1 is constitutively expressed in various tissues particularly in the green tissues. The label-free qualitative proteomics showed that photosynthesis-related pathways and ribosomal pathways were significantly inhibited in OsCpn60β1 mutants. These results indicate that OsCpn60β1 is essential for chloroplast development in rice.

scholarly journals Effects of Cyanamide Contained in Lime Nitrogen on Seed Dormancy and Germination ability of Rice (Oryza sativa L.)

2014 ◽  
Vol 83 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Youichi Ohdaira ◽  
Hiroyuki Shiratsuchi ◽  
Hiromichi Yamaguchi ◽  
Akari Fukuda
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L ◽  
Germination Ability ◽  
Seed Dormancy And Germination

Genetic dissection of the seed dormancy trait in cultivated rice (Oryza sativa L.)

Plant Science ◽  
2006 ◽  
Vol 170 (4) ◽  
pp. 786-792 ◽  
Author(s):  
J.M. Wan ◽  
L. Jiang ◽  
J.Y. Tang ◽  
C.M. Wang ◽  
M.Y. Hou ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Seed Dormancy ◽  
Oryza Sativa L ◽  
Genetic Dissection ◽  
Cultivated Rice
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