Maximizing Hydrogen Production and Substrate Consumption by Escherichia coli WDHL in Cheese Whey Fermentation

2013 ◽  
Vol 171 (3) ◽  
pp. 704-715 ◽  
Author(s):  
Luis Manuel Rosales-Colunga ◽  
Zazil Donaxí Alvarado-Cuevas ◽  
Elías Razo-Flores ◽  
Antonio De León Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Production ◽  
Cheese Whey ◽  
Substrate Consumption

scholarly journals Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate

2013 ◽  
Vol 30 (6) ◽  
pp. 585-590 ◽  
Author(s):  
Zazil D. Alvarado-Cuevas ◽  
Leandro G. Ordoñez Acevedo ◽  
José Tomás Ornelas Salas ◽  
Antonio De León-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Production ◽  
Cheese Whey ◽  
Nitrogen Sources

Hydrogen production by Escherichia coli ΔhycA ΔlacI using cheese whey as substrate

2010 ◽  
Vol 35 (2) ◽  
pp. 491-499 ◽  
Author(s):  
Luis Manuel Rosales-Colunga ◽  
Elías Razo-Flores ◽  
Leandro G. Ordoñez ◽  
Felipe Alatriste-Mondragón ◽  
Antonio De León-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Production ◽  
Cheese Whey

scholarly journals Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material

2020 ◽  
Vol 47 (12) ◽  
pp. 1117-1132
Author(s):  
Katharina Novak ◽  
Juliane Baar ◽  
Philipp Freitag ◽  
Stefan Pflügl
Keyword(s):  
Escherichia Coli ◽  
Cheese Whey ◽  
Strain Improvement ◽  
Defined Medium ◽  
Raw Material ◽  
Efficient Production ◽  
Substrate Uptake ◽  
Chemically Defined Medium ◽  
E Coli ◽  
Plasmid Library

AbstractThe aim of this study was to establish isobutanol production on chemically defined medium in Escherichia coli. By individually expressing each gene of the pathway, we constructed a plasmid library for isobutanol production. Strain screening on chemically defined medium showed successful production in the robust E. coli W strain, and expression vector IB 4 was selected as the most promising construct due to its high isobutanol yields and efficient substrate uptake. The investigation of different aeration strategies in combination with strain improvement and the implementation of a pulsed fed-batch were key for the development of an efficient production process. E. coli W ΔldhA ΔadhE Δpta ΔfrdA enabled aerobic isobutanol production at 38% of the theoretical maximum. Use of cheese whey as raw material resulted in longer process stability, which allowed production of 20 g l−1 isobutanol. Demonstrating isobutanol production on both chemically defined medium and a residual waste stream, this study provides valuable information for further development of industrially relevant isobutanol production processes.

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