Evaluation of Point Mutation Detection in Mycobacterium tuberculosis with Isoniazid Resistance Using Real-Time PCR and TaqMan Probe Assay

2014 ◽  
Vol 175 (5) ◽  
pp. 2447-2455 ◽  
Author(s):  
F. Riahi ◽  
M. Derakhshan ◽  
A. Mosavat ◽  
S. Soleimanpour ◽  
S. A. Rezaee
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Point Mutation ◽  
Real Time Pcr ◽  
Mutation Detection ◽  
Taqman Probe ◽  
Isoniazid Resistance ◽  
Probe Assay

Improved real-time PCR for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates

2003 ◽  
Vol 45 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Maria J Torres ◽  
Antonio Criado ◽  
Maite Ruiz ◽  
Ana C Llanos ◽  
Jose C Palomares ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Clinical Isolates ◽  
Isoniazid Resistance

scholarly journals Dual-Probe Assay for Rapid Detection of Drug-Resistant Mycobacterium tuberculosis by Real-Time PCR

2004 ◽  
Vol 42 (11) ◽  
pp. 5277-5285 ◽  
Author(s):  
T. Wada ◽  
S. Maeda ◽  
A. Tamaru ◽  
S. Imai ◽  
A. Hase ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Drug Resistant ◽  
Probe Assay ◽  
Dual Probe

scholarly journals DESAIN TAQMAN PROBE SECARA IN SILICO SEBAGAI PENDETEKSI MUTASI PADA KODON 516 GEN rpoB Mycobacterium tuberculosis UNTUK METODE REAL-TIME PCR

2017 ◽  
Vol 4 (1) ◽  
pp. 22
Author(s):  
Dek Pueteri Dewi Suryani ◽  
Putu Sanna Yustiantara ◽  
Sagung Chandra Yowani
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Aspartic Acid ◽  
Real Time Pcr ◽  
In Silico ◽  
Rpob Gene ◽  
Taqman Probe ◽  
Probe Design ◽  
Accession Number ◽  
Pcr Method

The aim of this research was to in silico design of TaqMan probe. Design of TaqMan probe were conducted using software Clone Manager Suite 6. As a template, the rpoB gene of M. tuberculosis H37Rv (accession number U12205.1) was used. The results of this research were 8 sequences such as, R516MV-2, R516MV-3, R516MV-4, R516MV-5, R516MV-7, R516MV-8, R516MV-11, R516MV-13. These sequences were met the criteria of TaqMan probe, such as length of nucleotide (23-28 nucleotide), Tm value (72ºC), %GC (50-58%), runs and repeats (?4 bases), dimer structure in accordance to the requirements and does not form hairpin structures. In addition, these sequences were met labeling criteria of TaqMan probe which are including the location of G bases and the number of G-C bases in sequences. Therefore, these sequences could be labeled by FAM (reporter) at 5' end and TAMRA (quencher) at 3' end. The conclusion of this research, the sequences were met the criteria of TaqMan probe. Therefore, it could be targeted to detect mutations at codon 516 with a change of aspartic acid into valine (GAC ? GTC) by using real-time PCR method.

A real-time PCR assay for detection of isoniazid resistance in Mycobacterium tuberculosis clinical isolates

2003 ◽  
Vol 55 (3) ◽  
pp. 797-800 ◽  
Author(s):  
Laura Rindi ◽  
Lea Bianchi ◽  
Enrico Tortoli ◽  
Nicoletta Lari ◽  
Daniela Bonanni ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Clinical Isolates ◽  
Pcr Assay ◽  
Isoniazid Resistance

scholarly journals Locked Nucleic Acid Probe-Based Real-Time PCR Assay for the Rapid Detection of Rifampin-Resistant Mycobacterium tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0143444 ◽  
Author(s):  
Yong Zhao ◽  
Guilian Li ◽  
Chongyun Sun ◽  
Chao Li ◽  
Xiaochen Wang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Locked Nucleic Acid Probe
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