DNA Methylation and Methylation Polymorphism in Genetically Stable In vitro Regenerates of Jatropha curcas L. Using Methylation-Sensitive AFLP Markers

ABSTRACT Jatropha curcas L. is a plant species with many potential applications, especially medicinal uses (hypoglycemic, anti-inflammatory, haemostatic, healing, anti-tumor). The objective of this study was to test germination in moist paper rolls for whole seeds and in vitro for excised embryonic axes, in an attempt to identify the best method to assess the quality of J. curcas seed germplasm, cryopreserved with different water contents. The experimental sample with a 6.2% moisture content (MC) was divided in subsamples which were hydrated and dehydrated for 0 (control), 4, 8, 11 and 24h. The initial germination percentages were 63% for whole seeds and 81% for excised embryonic axes. After exposure to liquid nitrogen (LN), germination percentages were 48% (whole seeds) and 57% (excised embryonic axes). There was no significant difference between germination percentages in embryonic excised from seeds subjected or not subjected to freezing, with different MC. In contrast, there was a reduction of the whole seed germination percentage when exposed to LN (contrast = 0.17, standard error = 0.04, t = 4.09, p = 0.001) and not for the hydration and dehydration treatments. The methodology based on in vitro cultures of the embryonic axis isolated from seeds stored in LN with distinct MC values was more efficient than the standard germination test to evaluate the viability of J. curcas seeds before and after LN storage.

Download Full-text

Induction of different types of callus in Jatropha curcas L. hybrid accession at in vitro condition

Australian Journal of Crop Science ◽

10.21475/ajcs.17.11.07.pne526 ◽

2017 ◽

pp. 874-879

Author(s):

Alexandre Bosco de Oliveira ◽

◽

Wagner A. Vendrame ◽

Luciana Cardoso Nogueira Londe ◽

Massy Sanaey ◽

...

Keyword(s):

Jatropha Curcas ◽

Jatropha Curcas L ◽

Different Types

Download Full-text

PENGARUH FRAKSI EKSTRAK DAUN NIMBA (Azadirachta indica A.) DAN DAUN JARAK (Jatropha curcas L.) TERHADAP DIAMETER KOLONI DAN JUMLAH SPORA JAMUR Colletotrichum capsici PENYEBAB PENYAKIT ANTRAKNOSA PADA CABAI (Capsicum annum L.)

Jurnal Agrotek Tropika ◽

10.23960/jat.v1i3.2059 ◽

2013 ◽

Vol 1 (3) ◽

Author(s):

Yanti Ningsih ◽

Efri Efri ◽

Titik Nur Aeny

Keyword(s):

Capsicum Annuum ◽

Azadirachta Indica ◽

Jatropha Curcas ◽

Jatropha Curcas L ◽

Capsicum Annum ◽

Colletotrichum Capsici ◽

Capsicum Annuum L

Tanaman cabai (Capsicum annuum L.) merupakan tanaman semusim yang tergolong dalam famili solanaceae. Budidaya cabai seringkali menghadapi banyak kendala terutama dalam usaha meningkatkan produktivitas, baik dalam hal kualitas maupun kuantitasnya. Salah satu penyakit yang menjadi kendala pada pertanaman cabai adalah penyakit antraknosa. Penyakit ini disebabkan oleh jamur Colletotrichum capsici dan pada tingkat tertentu dapat mengakibatkan kerugian yang cukup besar. Penelitian ini bertujuan untuk mengetahui efektivitas fraksi ekstrak daun nimba dan daun jarak sebagai biofungisida terhadap pertumbuhan C. capsici secara in vitro penyebab penyakit antraknosa pada cabai. Hasil penelitian menunjukkan bahwa ekstrak daun nimba fraksi alkohol 90% , ekstrak daun jarak fraksi alkohol 10%, fraksi alkohol 90%, fraksi etil asetat 10% dan fraksi n-heksana 90% berpotensi sebagai fungisida nabati yang dapat menghambat pertumbuhan koloni dan pembentukan spora C. capsici.

Download Full-text

Utilization of Endophytes Fungi from Jatropha Leaves (Jatropha curcas L.) Against Fusarium oxysporum Causing Tuber Rot Disease Onion (Allium cepa var. Ascalonicum (L.) Backer)

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.14.4.42 ◽

2020 ◽

Vol 14 (4) ◽

pp. 2655-2660

Author(s):

Akhmad Rizali ◽

Rabiatul Wahdah

Keyword(s):

Fusarium Oxysporum ◽

Natural Enemies ◽

Jatropha Curcas ◽

Plant Diseases ◽

Control Effort ◽

Growth Ratio ◽

Jatropha Curcas L ◽

Randomized Design ◽

Tuber Rot

Tuber rot is one of the important diseases in onion caused by Fusarium oxysporum. Endophytes are biological agents that are currently widely used in controlling plant diseases. Excessive use of pesticides and continuously has shown negative impacts such as resurgence, resistance to pests and pathogens, and the death of natural enemies. Currently, pests and pathogens control effort are directed at the utilization of natural enemies or better known as biological control. Endophyte is a microorganism that grows in plant tissues without causing symptoms. Endophytes allegedly capable of producing a variety of phytochemical compounds generated by their host. The leaves of Jatropha (Jatropha curcas L.) is one part of the plant that contains secondary metabolites.Phytochemical tests on the Jatropha show that Jatropha contains alkaloids, flavonoids, and saponins that are an antimicrobial potential of plant-pathogen control agents. This study aimed to determine the effect of some Endophytes isolates and their impact on the growth of F. oxysporum in vitro in onion.Exploration (endophyte isolation from Jatropha healthy leaves) and observation (antagonist test of Endophytes of the Jatropha leaves) method used in this study was a Completely Randomized Design (CRD) with a single factor (10 treatment) repeated three times. There were 14 endophytes isolated from Jatropha leaves with a growth ratio of 4.5 cm/2 days. The best treatment to inhibit the growth of F. oxysporum in vitro is Jc5, Jc8, Jc10.

Download Full-text