Immobilization of Recombinant Glucose Isomerase for Efficient Production of High Fructose Corn Syrup

2017 ◽  
Vol 183 (1) ◽  
pp. 293-306 ◽  
Author(s):  
Li-Qun Jin ◽  
Qi Xu ◽  
Zhi-Qiang Liu ◽  
Dong-Xu Jia ◽  
Cheng-Jun Liao ◽  
...  
Keyword(s):  
Glucose Isomerase ◽  
Efficient Production ◽  
High Fructose Corn Syrup ◽  
Corn Syrup ◽  
High Fructose

scholarly journals A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Chenxia Dai ◽  
Tingting Miao ◽  
Jinping Hai ◽  
Yunyi Xiao ◽  
Ying Li ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Cost Effective ◽  
Glucose Isomerase ◽  
Substrate Affinity ◽  
High Fructose Corn Syrup ◽  
Industrial Enzymes ◽  
Corn Syrup ◽  
Caldicellulosiruptor Bescii ◽  
High Fructose ◽  
The Cost

Glucose isomerase (GI) that catalyzes the conversion of D-glucose to D-fructose is one of the most important industrial enzymes for the production of high-fructose corn syrup (HFCS). In this study, a novel GI (CbGI) was cloned from Caldicellulosiruptor bescii and expressed in Escherichia coli. The purified recombinant CbGI (rCbGI) showed neutral and thermophilic properties. It had optimal activities at pH 7.0 and 80°C and retained stability at 85°C. In comparison with other reported GIs, rCbGI exhibited higher substrate affinity (Km=42.61 mM) and greater conversion efficiency (up to 57.3% with 3M D-glucose as the substrate). The high catalytic efficiency and affinity of this CbGI is much valuable for the cost-effective production of HFCS.

Safety Evaluation of Glucose Isomerase Derived from Flavobacterium arborescens and Used in Production of High Fructose Corn Syrup

1984 ◽  
Vol 47 (5) ◽  
pp. 359-371 ◽  
Author(s):  
MICHAEL C. PORTER ◽  
RALPH E. HARTNAGEL ◽  
ROBERT L. KOWALSKI ◽  
GEORGE R. CLEMENS ◽  
VENKATANARYANA JASTY ◽  
...  
Keyword(s):  
Crosslinking Agent ◽  
Safety Evaluation ◽  
Viable Cell ◽  
Glucose Isomerase ◽  
Exposure Level ◽  
High Fructose Corn Syrup ◽  
Biochemical Tests ◽  
Corn Syrup ◽  
High Fructose ◽  
Polycationic Polymers

Flavobacterium arborescens is a common rod-shaped, gram-negative bacterium which, when cultivated in a nutrient medium, is an efficient source of glucose isomerase (GI). GI is then used in the production of high fructose corn syrup. Studies were conducted to assure product safety and establish GRAS status for GI derived from F. arborescens. A viable cell suspension of F. arborescens and the cell-free medium in which the organism was cultured were administered i.v. to rats and rabbits. For feeding studies, the cells were immobilized using polycationic polymers and a crosslinking agent (i.e., chitosan, polyethylenimine and glutaraldehyde). GI, in the whole cell immobilized form, was offered at concentrations of 0, 1.5, 3.0 or 5.0% (wt/wt) of the diet to dogs for a minimum of 90 consecutive days and to rats over three generations. Animals were observed daily for signs of toxicosis; body weight and food consumption were monitored; biochemical tests, hematologic determinations, and urinalyses were done on blood and urine samples; and thorough gross and microscopic tissue examinations were performed at terminations. There were no signs of infection or toxicosis following i.v. administration of F. arborescens or the cell-free supernatant fluid. This, and the lack of toxicity in dogs and rats which received daily dietary concentrations of GI many times above the projected highest possible human exposure level, suggest that there should be virtually no risk of toxicity associated with the consumption of food and beverages containing high fructose syrup produced by GI derived from F. arborescens.

High-level expression of Thermobifida fusca glucose isomerase for high fructose corn syrup biosynthesis

2020 ◽  
Vol 135 ◽  
pp. 109494
Author(s):  
Fucheng Zhu ◽  
Hui Deng ◽  
Xiaomei He ◽  
Xiangwen Song ◽  
Naifu Chen ◽  
...  
Keyword(s):  
Glucose Isomerase ◽  
Thermobifida Fusca ◽  
High Fructose Corn Syrup ◽  
High Level Expression ◽  
Corn Syrup ◽  
High Fructose ◽  
High Level

Fructose and high fructose corn syrup: are they a two-edged sword?

2021 ◽  
pp. 1-23
Author(s):  
Nasim Khorshidian ◽  
Mahdi Shadnoush ◽  
Maryam Zabihzadeh Khajavi ◽  
Sara Sohrabvandi ◽  
Mojtaba Yousefi ◽  
...  
Keyword(s):  
High Fructose Corn Syrup ◽  
Corn Syrup ◽  
High Fructose
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