Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor

2021 ◽  
Author(s):  
Li-Qun Jin ◽  
Xian-Xiao Chen ◽  
Yi-Ting Jin ◽  
Jun-Kang Shentu ◽  
Zhi-Qiang Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Packed Bed ◽  
Glucose Isomerase ◽  
Packed Bed Reactor ◽  
Recombinant Escherichia Coli ◽  
High Fructose Corn Syrup ◽  
Corn Syrup ◽  
Escherichia Coli Cells ◽  
High Fructose ◽  
Modified Diatomite

R-mandelic acid production with immobilized recombinant Escherichia coli cells in a recirculating packed bed reactor

2016 ◽  
Vol 34 (5) ◽  
pp. 205-211 ◽  
Author(s):  
Xin-Hong Zhang ◽  
Zhi-Qiang Liu ◽  
Ya-Ping Xue ◽  
Bo Yang ◽  
Ming Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Production ◽  
Mandelic Acid ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Recombinant Escherichia Coli ◽  
Escherichia Coli Cells

scholarly journals KINETIC STUDY COMPARISON OF IMMOBILIZED GLUCOSE ISOMERASE (GENSWEET AND SWEETZYME IT) IN STIRRED TANK REACTOR AND PACKED BED REACTOR

2021 ◽  
Vol 10 (4) ◽  
pp. 3115-3119
Author(s):  
Debabrata Bera
Keyword(s):  
Enzyme Activity ◽  
Packed Bed ◽  
Glucose Isomerase ◽  
Packed Bed Reactor ◽  
Stirred Tank ◽  
High Fructose Corn Syrup ◽  
Enzymatic Conversion ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
High Fructose

D- Glucose/xylose isomerase catalysis the reversible isomerization of aldoses to ketoses such as D-glucose and D-xylose to D-fructose and D-xylose respectively. High fructose corn syrup (HFCS), a low calorie sugar substitute for cane sugar, utilizes Glucose isomerase enzyme for conversion of glucose to fructose. The conversion of glucose to fructose favours more at high temperature, providing an incentive to utilize thermostable and thermoactive glucose isomerase in High fructose corn syrup (HFCS) production. Present studies emphasize on enzymatic conversion and optimization using Sweetzyme IT extra & Gensweet, commercially available glucose isomerases. The experiments were carried out for enzymatic conversion of glucose to fructose using Gensweet and Sweetzyme in Packed bed reactor (PBR) and Stirred tank reactor (STR). Maximum conversion was seen in Stirred tank reactor (STR) using both of these enzymes, approx 10 % more Fructose conversion comparing it to packed bed reactor (PBR). Also, Stirred tank reactor (STR) reaction conditions such as pH, buffers, cofactor (MgSO4) requirement were optimized to achieve optimum enzyme activity. Analysis of enzymatic conversion samples was done using HPLC-RID (using Zorbax Column). The importance of the divalent cation MgCl2 for optimal enzyme activity was investigated. The enzyme performed best at pH 7.5 and 60°C, using 10mM MgSO4 as a cofactor. Utilizing Gensweet in Stirred tank reactor (STR), the maximum fructose transformation was 44 %. The most activity was detected with Sodium phosphate buffers, and EPPS buffers at pH 7 and 8, accordingly, whereas the least activity was reported with TRIS HCl buffer.

scholarly journals A Novel Glucose Isomerase from Caldicellulosiruptor bescii with Great Potentials in the Production of High-Fructose Corn Syrup

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Chenxia Dai ◽  
Tingting Miao ◽  
Jinping Hai ◽  
Yunyi Xiao ◽  
Ying Li ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Cost Effective ◽  
Glucose Isomerase ◽  
Substrate Affinity ◽  
High Fructose Corn Syrup ◽  
Industrial Enzymes ◽  
Corn Syrup ◽  
Caldicellulosiruptor Bescii ◽  
High Fructose ◽  
The Cost

Glucose isomerase (GI) that catalyzes the conversion of D-glucose to D-fructose is one of the most important industrial enzymes for the production of high-fructose corn syrup (HFCS). In this study, a novel GI (CbGI) was cloned from Caldicellulosiruptor bescii and expressed in Escherichia coli. The purified recombinant CbGI (rCbGI) showed neutral and thermophilic properties. It had optimal activities at pH 7.0 and 80°C and retained stability at 85°C. In comparison with other reported GIs, rCbGI exhibited higher substrate affinity (Km=42.61 mM) and greater conversion efficiency (up to 57.3% with 3M D-glucose as the substrate). The high catalytic efficiency and affinity of this CbGI is much valuable for the cost-effective production of HFCS.

Safety Evaluation of Glucose Isomerase Derived from Flavobacterium arborescens and Used in Production of High Fructose Corn Syrup

1984 ◽  
Vol 47 (5) ◽  
pp. 359-371 ◽  
Author(s):  
MICHAEL C. PORTER ◽  
RALPH E. HARTNAGEL ◽  
ROBERT L. KOWALSKI ◽  
GEORGE R. CLEMENS ◽  
VENKATANARYANA JASTY ◽  
...  
Keyword(s):  
Crosslinking Agent ◽  
Safety Evaluation ◽  
Viable Cell ◽  
Glucose Isomerase ◽  
Exposure Level ◽  
High Fructose Corn Syrup ◽  
Biochemical Tests ◽  
Corn Syrup ◽  
High Fructose ◽  
Polycationic Polymers

Flavobacterium arborescens is a common rod-shaped, gram-negative bacterium which, when cultivated in a nutrient medium, is an efficient source of glucose isomerase (GI). GI is then used in the production of high fructose corn syrup. Studies were conducted to assure product safety and establish GRAS status for GI derived from F. arborescens. A viable cell suspension of F. arborescens and the cell-free medium in which the organism was cultured were administered i.v. to rats and rabbits. For feeding studies, the cells were immobilized using polycationic polymers and a crosslinking agent (i.e., chitosan, polyethylenimine and glutaraldehyde). GI, in the whole cell immobilized form, was offered at concentrations of 0, 1.5, 3.0 or 5.0% (wt/wt) of the diet to dogs for a minimum of 90 consecutive days and to rats over three generations. Animals were observed daily for signs of toxicosis; body weight and food consumption were monitored; biochemical tests, hematologic determinations, and urinalyses were done on blood and urine samples; and thorough gross and microscopic tissue examinations were performed at terminations. There were no signs of infection or toxicosis following i.v. administration of F. arborescens or the cell-free supernatant fluid. This, and the lack of toxicity in dogs and rats which received daily dietary concentrations of GI many times above the projected highest possible human exposure level, suggest that there should be virtually no risk of toxicity associated with the consumption of food and beverages containing high fructose syrup produced by GI derived from F. arborescens.

scholarly journals Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor

2020 ◽  
Vol 309 ◽  
pp. 125710 ◽  
Author(s):  
Sawssan Neifar ◽  
Fadia V. Cervantes ◽  
Amel Bouanane-Darenfed ◽  
Hajer BenHlima ◽  
Antonio O. Ballesteros ◽  
...  
Keyword(s):  
Packed Bed ◽  
Glucose Isomerase ◽  
Packed Bed Reactor ◽  
High Fructose ◽  
High Fructose Syrup

High-level expression of Thermobifida fusca glucose isomerase for high fructose corn syrup biosynthesis

2020 ◽  
Vol 135 ◽  
pp. 109494
Author(s):  
Fucheng Zhu ◽  
Hui Deng ◽  
Xiaomei He ◽  
Xiangwen Song ◽  
Naifu Chen ◽  
...  
Keyword(s):  
Glucose Isomerase ◽  
Thermobifida Fusca ◽  
High Fructose Corn Syrup ◽  
High Level Expression ◽  
Corn Syrup ◽  
High Fructose ◽  
High Level
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