Enhancement of Copper Uptake of Yeast Through Systematic Optimization of Medium and the Cultivation Process of Saccharomyces cerevisiae

2022 ◽  
Author(s):  
Xue-Na Guo ◽  
Xiao-Xian He ◽  
Li-Bin Zhang ◽  
Yan-Fei Cheng ◽  
Xiu-Mei Bai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Copper Uptake ◽  
Systematic Optimization ◽  
Cultivation Process

The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1

Microbiology ◽  
2004 ◽  
Vol 150 (7) ◽  
pp. 2197-2208 ◽  
Author(s):  
Marcus E. Marvin ◽  
Robert P. Mason ◽  
Annette M. Cashmore
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Iron Uptake ◽  
Invasive Growth ◽  
Hostile Environment ◽  
Null Mutant ◽  
Copper Uptake ◽  
Similar System ◽  
Copper Transporter ◽  
High Affinity ◽  
Null Strain

The ability of Candida albicans to acquire iron from the hostile environment of the host is known to be necessary for virulence and appears to be achieved using a similar system to that described for Saccharomyces cerevisiae. In S. cerevisiae, high-affinity iron uptake is dependent upon the acquisition of copper. The authors have previously identified a C. albicans gene (CaCTR1) that encodes a copper transporter. Deletion of this gene results in a mutant strain that grows predominantly as pseudohyphae and displays aberrant morphology in low-copper conditions. This paper demonstrates that invasive growth by C. albicans is induced by low-copper conditions and that this is augmented in a Cactr1-null strain. It also shows that deletion of CaCTR1 results in defective iron uptake. In S. cerevisiae, genes that facilitate high-affinity copper uptake are controlled by a copper-sensing transactivator, ScMac1p. The authors have now identified a C. albicans gene (CaMAC1) that encodes a copper-sensing transactivator. A Camac1-null mutant displays phenotypes similar to those of a Cactr1-null mutant and has no detectable CaCTR1 transcripts in low-copper conditions. It is proposed that high-affinity copper uptake by C. albicans is necessary for reductive iron uptake and is transcriptionally controlled by CaMac1p in a similar manner to that in S. cerevisiae.

scholarly journals Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae

Open Biology ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 150223 ◽  
Author(s):  
Katherine E. Vest ◽  
Jing Wang ◽  
Micah G. Gammon ◽  
Margaret K. Maynard ◽  
Olivia L. White ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transport Systems ◽  
Growth Defect ◽  
Intermembrane Space ◽  
Copper Uptake ◽  
Copper Sensor ◽  
The Matrix ◽  
Mitochondrial Carrier Family ◽  
Dependent Growth ◽  
Copper Import

In Saccharomyces cerevisiae , the mitochondrial carrier family protein Pic2 imports copper into the matrix. Deletion of PIC2 causes defects in mitochondrial copper uptake and copper-dependent growth phenotypes owing to decreased cytochrome c oxidase activity. However, copper import is not completely eliminated in this mutant, so alternative transport systems must exist. Deletion of MRS3 , a component of the iron import machinery, also causes a copper-dependent growth defect on non-fermentable carbon. Deletion of both PIC2 and MRS3 led to a more severe respiratory growth defect than either individual mutant. In addition, MRS3 expressed from a high copy number vector was able to suppress the oxygen consumption and copper uptake defects of a strain lacking PIC2 . When expressed in Lactococcus lactis , Mrs3 mediated copper and iron import. Finally, a PIC2 and MRS3 double mutant prevented the copper-dependent activation of a heterologously expressed copper sensor in the mitochondrial intermembrane space. Taken together, these data support a role for the iron transporter Mrs3 in copper import into the mitochondrial matrix.

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