The Effect of Nutrients Mixture on The Biomass and Lipid Production from Microalgae Botryococcus braunii Mutated by UV-C Rays

Nutrient is one of the most important factors in the growth of microalgae. This research was conducted to study the effect of nutrient mixture on the biomass and lipid production of Botryococcus braunii. Microalgae B. braunii was cultivated in the commercial nutrient medium of agricultural fertilizer combinations of ammonium sulphate (ZA), urea, and triple superphosphate (TSP). Before the cultivation process, B. braunii was exposed to UV-C rays (254 nm) for 3 minutes. The concentration and type of fertilizer as a nitrogen source divided into four types of mixtures, namely FM-1, FM-2, FM-3, and FM-4 were compared with Walne nutrients to study their effects on microalgae growth and lipids. FM-1 consisting of 150 mg/L of ZA, 7.5 mg/L of urea, and 25 mg/L of TSP led to the best growth for native and mutated microalgae strains compared to Walne nutrients and other nutrient mixtures. The mutated microalgae showed less growth than the native microalgae strains. However, the mutation process significantly increased the lipid content in the microalgae. In native microalgae strains, FM-4 consisting of 136.3 mg/L of urea and 50 mg/L of TSP produced the lowest lipid at 8.96%. After being exposed to UV-C rays, the lipids in FM-4 medium increased to 55.11%. The results show that the use of commercial fertilizers and exposure to UV-C rays on microalgae have high potential in preparing lipids as raw material for biodiesel which can be effectively applied in large-scale microalgae cultivation.

BUDI DAYA KELAPA DAN PEMASARAN KOPRA DI BUOL 1970-2019

As the price of copra increased in the global market, coconut cultivation in Buol received great attention in the early 20th century. During the New Order, the government paid attention again to people’s plantations, especially coconuts. Coconut cultivation was promoted in several areas, including Buol. This paper scrutinizes the cultivation process and development of copra production in Buol. The commodities were closely related because the cultivation process greatly affected the production of copra. This study used the historical method to suggest that the people of Buol were cultivating coconut because of its great benefits, including how easy it was to be processed. In addition, other findings of this study indicate that the price of copra at the plantation level was determined by interconnected factors such as the quality of copra, the marketing network, and the absence of koperasi (economic enterprise) as a market counterweight. The study also found that although they were seen as economic symbols for the Buolese, these two commodities did not contribute significantly to the welfare of Buolese, particularly the small farmers who do not have vast cultivating lands.

A report on characterization of submerged fermentation of Phellinus linteus in Vietnam

Phellinus linteus strain GC was cultured for harvesting biomass by submerged fermentation method in 100 L fermenter which produced in Vietnam. The process of cultivation of this fungus was performed in aerobic conditions with aeration at 1 vvm, stirred at 150 rpm, temperature from 27oC to 29oC. The observation of cultivation was performed to evaluate the optimum time of growth which produce highest biomass of the mycelium for application purpose of using as functional food. The pH of medium was slightly changed with decrease then increase again, and reached about 6.0 when cultivation process ends. The biomass of mycelium was gradually increased until about 11 days of cultivation with about 30 g/L of dried biomass and the content of of sugar reduced in medium decreased from 40 g/L to 6 g/L.

Analisis Kualitatif Residu Pestisida Pada Bahan Pangan Dengan Menggunakan Metode Kromatografi Lapis Tipis (KLT)

Vegetables and fruits are foodstuffs whose cultivation process requires pesticides. In addition to farmers who apply pesticides, pesticide poisoning can also be experienced by people who consume agricultural products including vegetables through the pesticide residues contained in them, so that it will have a negative impact on human health who consume them continuously. The purpose of the study was to determine the presence of pesticide residues in foodstuffs. This research is a laboratory observation with a simple random sampling technique. In this study, a qualitative analysis of organophosphate and carbamate pesticide residues in food ingredients was carried out using the Thin Layer Chromatography (TLC) method. Based on the results of the study, from 15 (fifteen) samples of vegetables, no organophosphate chlorpyrifos pesticide content was detected, but the carbamate group was detected in samples of spinach, cabbage, and carrots marked with spots and the Rf value was the same as the Furadan standard. Thus, it is hoped that the community will wash the vegetables before they are processed and consumed.

PENERAPAN TEKNOLOGI ALAT PENANAM BENIH JAGUNG TIPE ROW SEEDER DI KELOMPOK TANI SUKA MAJU UNTUK MENDUKUNG KETAHANAN PANGAN WILAYAH PERBATASAN KALIMANTAN UTARA

ABSTRAKPermasalahan yang dihadapi oleh Kelompok tani Suka Maju hingga saat ini yaitu masih menggunakan metode manual dalam proses penanaman benih jagung, hal ini membuat waktu pengerjaan lebih lama dan posisi lubang tidak sejajar dan presisi. Dampak dari dua hal tersebut yaitu akan menambah biaya tenaga kerja dan posisi jagung yang tidak sejajar ataupun berhimpitan akan membuat petumbuhan tanaman jagung tidak optimal. Pengabdian ini bertujuan untuk menerapkan teknologi alat penanam benih tipe row seeder untuk mengurangi jerih kerja petani pada saat proses penanaman benih khususnya tanaman jagung. Kegiatan PKM ini dilakukan di lahan Kelompok tani Suka Maju Kelurahan Juata Laut Kecamatan Tarakan Utara, Kota Tarakan. Kegiatan ini dibagi dalam beberapa tahapan yaitu survey, sosialisasi, pembuatan alat, bimbingan teknis serta evaluasi pelaksanaan dan keberlanjutan program oleh Kelompok Tani. Kegiatan PKM penerapan teknologi alat penanam tipe row seeder di kelompok tani Suka Maju berjalan dengan baik serta para petani bersemangat untuk mengaplikasikan alat penanam di setiap lahan mereka. Selain itu terjadi peningkatan efisiensi dalam proses budidaya tanaman jagung dikelompok tani suka maju yaitu proses penananam jagung bisa lebih cepat 45% dibandingkan pada saat penanaman benih jagung secara manual Kata kunci: penerapan; alat penanam benih jagung; tipe row seeder. ABSTRACTThe problem faced by the Suka Maju farmer group until now is that they still use manual methods in the process of planting corn seeds, this makes the processing time longer and the position of the planting holes is not parallel and precise. The impact of these two things is that it will increase labor costs and the position of corn that is not parallel or coincides will make corn plant growth not optimal. This service aims to apply row seeder type seed planter technology to reduce the labor of farmers during the seed planting process, especially for corn plants. This PKM activity was carried out on agricultural land belonging to the Suka Maju farmer group, Juata Laut Village, North Tarakan District, Tarakan City. This activity is divided into several stages, namely survey, socialization, tool making, technical guidance and evaluation of the implementation and sustainability of the program by the Farmer Group. The PKM activity for the application of row seeder type planter technology in the Suka Maju farmer group is going well and the farmers are excited to apply the planter in each of their lands. In addition, there is an increase in efficiency in the corn cultivation process in the advanced farmer group, namely the corn planting process can be 45% faster than when planting corn seeds manually. Keywords: application; corn seed planter; row seeder type.

Formation of Streptomyces protoplasts during cultivation in liquid media with lytic enzyme

Many streptomycetes strains are hardly or not at all transformable via protoplasts, or there is a problem with the regeneration of protoplasts. We found that protoplasts are formed directly in cultivation media under submerged conditions in the presence of lytic enzyme. Actinophage μ1/6 endolysin and lysozyme were used in this study. Streptomyces strains were cultivated in several media with glycine and lytic enzyme for 24 and 48h. The highest amounts of protoplasts (about 3 x 107 cfu/ml of cultivation medium) together with the highest regeneration (95%) and transformation frequency (about 2 x 106 – 107 cfu/μg DNA) were obtained reproducibly in YEME medium with high sucrose content. S. aureofaciens B96, as hardly transformable strain because of difficulties with protoplast preparation and their further regeneration, was used in this study. The same procedure was applied to S. lividans 66 TK24 and S. coelicolor A3(2), streptomycetes model strains, to confirm the general use of this method. Moreover, such cultivation process was appropriate for additional quick isolation of either chromosomal as well as plasmid DNA that could be further used in recombinant DNA techniques.

A biosurfactant-producing yeast Rhodotorula sp.CC01 utilizing landfill leachate as nitrogen source and its broad degradation spectra of petroleum hydrocarbons

In this study, a biosurfactant producing strain, Rhodotorula sp. CC01 was isolated using landfill leachate as nitrogen source, while olive oil was determined as the best sole carbon source for producing biosurfactants. The biosurfactant produced by Rhodotorula sp. CC01 was characterized as glycolipids with a critical micelle concentration of 70 mg/L, which showed stability over a wide range of pH (2–12), salinity (0–100%), and temperature (20–100°C). During the cultivation process, the surface tension decreased from 51.87 to 28.20 mN/m in 15 h, and the removal efficiency of NH4+-N reached 84.2% after 75 h cultivation with a maximum NH4+-N removal rate of 3.92 mg·L-1·h−1. In addition, Rhodotorula sp. CC01 has proven to be of great potential in remediating petroleum hydrocarbons, as revealed by chromogenic assays. The findings of this study prove a cost-effective strategy for the production of BS by yeast through the utilization of landfill leachate.

Construction of mathematical modelling of a population of microalgae

This article devoted to the development of the mathematic model of the technological process of the chlorella cultivation process, its features and solving of this mathematic model. The Exponential growth of microalgae population under conditions of unlimited nutrient resources and population space proceeds at a rate proportional to the number of species of predominant cells and is described by the differential equation. In the presence of several inhibitors, specific velocity equations with the number of inhibitors can be used, but, as a rule, there are practically no elements acting as inhibitors in the cultivation of Chlorella microalgae. The modeling of this particular class of objects did not take into account the effect of inhibitors on the growth of microalgae. The consumption of nutrients to support the life of microalgae is described by the differential equation. In the course of this work, the processes of cultivation of microalgae were brought together into a system of equations. As a result, a system of differential equations of the technological process of Chlorella cultivation was obtained. Thus, the obtained system of equations describes the process of cultivation of microalgae and its technological process, implemented in a periodic mode.