How to report the antinuclear antibodies (anti-cell antibodies) test on HEp-2 cells: guidelines from the ICAP initiative

Mice of the inbred NZB strain develop a spontaneous disease characterized by autoimmune hemolytic anemia, positive lupus erythematosus cell tests and antinuclear antibodies and nephritis. This disease is analogous to human systemic lupus erythematosus. In ultrastructural studies of the glomerular lesion in NZB mice, intraglomerular dense deposits in mesangial, subepithelial and subendothelial locations were described. In common with the findings in many examples of human and experimental nephritis, including many cases of human lupus nephritis, these deposits were amorphous or slightly granular in appearance with no definable substructure.We have recently observed structured deposits in the glomeruli of NZB mice. They were uncommon and were found in older animals with severe glomerular lesions by morphologic criteria. They were seen most commonly as extracellular elements in subendothelial and mesangial regions. The deposits ranged up to 3 microns in greatest dimension and were often adjacent to deposits of lipid-like round particles of 30 to 250 millimicrons in diameter and with amorphous dense deposits.

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LE cell test and antinuclear antibodies

JAMA ◽

10.1001/jama.200.12.1053 ◽

1967 ◽

Vol 200 (12) ◽

pp. 1053-1054 ◽

Cited By ~ 2

Author(s):

E. L. Dubois

Keyword(s):

Antinuclear Antibodies ◽

Cell Test

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Antinuclear Antibodies in both Healthy Individuals and Subjects with Raynaud's Phenomenon.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.55.915 ◽

1993 ◽

Vol 55 (5) ◽

pp. 915-919 ◽

Cited By ~ 1

Author(s):

Yoko KOMURA ◽

Hideyo YOSHIDA ◽

Setsuya FUJITA ◽

Ryoichi INABA ◽

Hirotoshi IWATA

Keyword(s):

Antinuclear Antibodies ◽

Raynaud’S Phenomenon ◽

Raynaud's Phenomenon ◽

Healthy Individuals

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FLUORESCENCE STAINING PATTERNS AND TITRE OF ANTINUCLEAR ANTIBODIES IN ELDERLY PATIENTS

10.26226/morressier.57bc1756d462b80290b4d7c2 ◽

2016 ◽

Author(s):

Deleted Account

Keyword(s):

Elderly Patients ◽

Antinuclear Antibodies ◽

Fluorescence Staining

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Canine Antinuclear Antibodies: Comparison of Immunofluorescence Staining Patterns and Precipitin Reactivity

Acta Veterinaria Scandinavica ◽

10.1186/bf03547018 ◽

1999 ◽

Vol 40 (3) ◽

pp. 205-212

Author(s):

H. Hansson ◽

A. Karlsson-Parra

Keyword(s):

Antinuclear Antibodies ◽

Immunofluorescence Staining

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Raynaud's phenomenon and positive antinuclear antibodies in a malignancy.

Annals of the Rheumatic Diseases ◽

10.1136/ard.49.11.935 ◽

1990 ◽

Vol 49 (11) ◽

pp. 935-936 ◽

Cited By ~ 12

Author(s):

C Tolosa-Vilella ◽

J Ordi-Ros ◽

M Vilardell-Tarres ◽

A Selva-O'Callaghan ◽

R Jordana-Comajuncosa

Keyword(s):

Antinuclear Antibodies ◽

Raynaud’S Phenomenon ◽

Raynaud's Phenomenon

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Evaluation of the performance of immunoblot and immunodot techniques used to identify autoantibodies in patients with autoimmune diseases

Open Chemistry ◽

10.1515/chem-2020-0101 ◽

2021 ◽

Vol 19 (1) ◽

pp. 237-244

Author(s):

Youssef EL Hassouni ◽

Mohammed Bourhia ◽

Ahmed Bari ◽

Riaz Ullah ◽

Hafiz Majid Mahmood ◽

...

Keyword(s):

Immune System ◽

Autoimmune Diseases ◽

Indirect Immunofluorescence ◽

Antinuclear Antibodies ◽

Pathological Conditions ◽

Nuclear Antigens ◽

Significant Difference ◽

U1 Snrnp

Abstract Autoimmune diseases are pathological conditions in which the immune system mistakenly attacks its own tissues. This study evaluates the performance of two techniques, which are identifiers of autoantibody specifics: immunoblot and immunodot. This study was conducted in 300 patients of whom 62 were tested positive for antinuclear antibodies. The patients were initially screened for antinuclear antibodies using indirect immunofluorescence. Then, the identification of specific autoantibodies such as anti-extractable nuclear antigens (ENAs) was carried out using the immunoblot and immunodot techniques. The results showed that immunoblot and immunodot did not present a significant difference in their sensitivity against anti-SSA/52, SSB, CENP-B, PCNA, U1-snRNP, Jo-1, Pm-scl, and Mi-2 (p > 0.05). However, the two techniques showed a significant difference in their sensitivity toward autoantibodies anti-DNAn, anti-histone, anti-SmD1, and anti-ds-DNA (p < 0.05). The immunoblot data were in complete accordance with the immunodot data (100%) regarding the detection of autoantibodies such as anti SSA/52, SSB, CENP-B, PCNA, U1-snRP, Jo-1, Pm-scl, and Mi-2, 80% regarding SmD1, and 75% concerning ds-DNA. We should certainly pay closer attention to the efficiency of the techniques used in the diagnosis of autoimmune diseases.

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Biologics modulate antinuclear antibodies, immunoglobulin E, and eosinophil counts in psoriasis patients

The Journal of Dermatology ◽

10.1111/1346-8138.16102 ◽

2021 ◽

Author(s):

Riichiro Sugiura ◽

Hitoshi Terui ◽

Ryoko Shimada‐Omori ◽

Emi Yamazaki ◽

Kenichiro Tsuchiyama ◽

...

Keyword(s):

Antinuclear Antibodies ◽

Immunoglobulin E ◽

Eosinophil Counts

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Clinical and immunological characteristics of 150 systemic lupus erythematosus patients in Jamaica: a comparative analysis

Lupus ◽

10.1177/0961203317707828 ◽

2017 ◽

Vol 26 (13) ◽

pp. 1448-1456 ◽

Cited By ~ 4

Author(s):

K C Maloney ◽

T S Ferguson ◽

H D Stewart ◽

A A Myers ◽

K De Ceulaer

Keyword(s):

Systemic Lupus Erythematosus ◽

Lupus Nephritis ◽

Disease Activity ◽

Renal Biopsy ◽

Diagnostic Criteria ◽

Lupus Erythematosus ◽

Antinuclear Antibodies ◽

Damage Index ◽

Systemic Lupus ◽

Dsdna Antibodies

Background Epidemiological studies in systemic lupus erythematosus have been reported in the literature in many countries and ethnic groups. Although systemic lupus erythematosus in Jamaica has been described in the past, there has not been a detailed evaluation of systemic lupus erythematosus patients in urban Jamaica, a largely Afro-Caribbean population. The goal of this study was to describe the clinical features, particularly disease activity, damage index and immunological features, of 150 systemic lupus erythematosus subjects. Methods 150 adult patients (≥18 years) followed in rheumatology clinic at a tertiary rheumatology hospital centre (one of two of the major public referral centres in Jamaica) and the private rheumatology offices in urban Jamaica who fulfilled Systemic Lupus International Collaborating Clinics (SLICC) criteria were included. Data were collected by detailed clinical interview and examination and laboratory investigations. Hence demographics, SLICC criteria, immunological profile, systemic lupus erythematosus disease activity index 2000 (SLEDAI-2K) and SLICC/American College of Rheumatology (ACR) damage index (SDI) were documented. Results Of the 150 patients, 145 (96.7%) were female and five (3.3%) were male. The mean age at systemic lupus erythematosus onset was 33.2 ± 10.9. Mean disease duration was 11.3 ± 8.6 years. The most prevalent clinical SLICC criteria were musculoskeletal, with 141 (94%) of subjects experiencing arthralgia/arthritis, followed by mucocutaneous manifestations of alopecia 103 (68.7%) and malar rash 46 (30.7%), discoid rash 45 (30%) and photosensitivity 40 (26.7%). Lupus nephritis (biopsy proven) occurred in 42 (28%) subjects and 25 (16.7%) met SLICC diagnostic criteria with only positive antinuclear antibodies/dsDNA antibodies and lupus nephritis on renal biopsy. The most common laboratory SLICC criteria were positive antinuclear antibodies 136 (90.7%) followed by anti-dsDNA antibodies 95 (63.3%) and low complement (C3) levels 38 (25.3%). Twenty-seven (18%) met SLICC diagnostic criteria with only positive antinuclear antibodies/anti-dsDNA antibodies and lupus nephritis on renal biopsy. Mean SLEDAI score was 6.9 ± 5.1 with a range of 0–32. Organ damage occurred in 129 (86%) patients; mean SDI was 2.4 ± 1.8, with a range of 0–9. Conclusion These results are similar to the clinical manifestations reported in other Afro-Caribbean populations; however, distinct differences exist with respect to organ involvement and damage, particularly with respect to renal involvement, which appears to be reduced in our participants.

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