Activating Transcription Factor 6 Contributes to Functional Recovery After Spinal Cord Injury in Adult Zebrafish

2020 ◽  
Author(s):  
Zhe Ji ◽  
Zhi-Lan Zhou ◽  
Qin Hao ◽  
Lin Zhao ◽  
Chun Cui ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Transcription Factor ◽  
Functional Recovery ◽  
Adult Zebrafish ◽  
Cord Injury ◽  
Activating Transcription Factor

scholarly journals The Asparaginyl Endopeptidase Legumain Is Essential for Functional Recovery after Spinal Cord Injury in Adult Zebrafish

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e95098 ◽  
Author(s):  
Liping Ma ◽  
Yan-Qin Shen ◽  
Harsh P. Khatri ◽  
Melitta Schachner
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Adult Zebrafish ◽  
Asparaginyl Endopeptidase ◽  
Cord Injury

scholarly journals Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord injury

2020 ◽  
Author(s):  
Fusheng Zhao ◽  
Geng Wu ◽  
Yang Wu ◽  
Yunlong Bai ◽  
Yongjia Qiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Endoplasmic Reticulum ◽  
Transcription Factor ◽  
Endoplasmic Reticulum Stress ◽  
Rating Scale ◽  
Motor Dysfunction ◽  
Cord Injury ◽  
Activating Transcription Factor

Abstract Background: Spinal cord injury (SCI) is a severe neurological disorder for which there is currently no effective treatment. Electroacupuncture (EA) is a means of combining traditional acupuncture with modern electrotherapy, which has been widely used and verified to have neuroprotective effects. The aim of this study was to evaluate the effects of EA treatment on the repair of SCI and to investigate the possible mechanisms. Methods: Rats were randomly divided into sham, sham+EA, SCI and SCI+EA four groups after SCI model was established. Rat motor function was assessed by the Basso, Beattie and Bresnahan locomotor rating scale, inclined plane test and footprint analysis. Histological alterations were examined with hematoxylin-eosin and Nissl staining. Oxidative stress was evaluated by measuring reactive oxygen species (ROS), glutathione (GSH), total antioxidant capacity (T-AOC), 3-nitrotyrosine (3-NT), as well as 4-hydroxynonenal (4-HNE) levels. The expression of p66Shc and endoplasmic reticulum stress (ERS) were detected to explore the involved mechanisms.Results: EA treatment significantly improved motor functional recovery, reduced spinal cord lesion cavity and neuronal chromatolysis after SCI. Meanwhile, EA treatment alleviated oxidative stress, as indicated by suppression of ROS production, increase in GSH and T-AOC levels and reduction of 3-NT and 4-HNE expression. Further, EA stimulation markedly eliminated the aberrant increase of p66Shc due to SCI in rats. More notably, EA treatment also attenuated ERS via down-regulation of glucose-regulated protein 78, activating transcription factor 4, C/EBP homologous protein, X-box binding protein 1 and activating transcription factor 6 expression in rat spinal cord tissues after SCI. Conclusions: These findings suggest that EA is a potential strategy for treatment of SCI, and the mechanism might be, at least in part, associated with mitigation of p66Shc-mediated oxidative stress and ERS in rats.

scholarly journals Electroacupuncture Ameliorates Motor Dysfunction via Inhibiting p66Shc-Associated Oxidative Stress and Endoplasmic Reticulum Stress in Rats with Spinal Cord Injury

2020 ◽  
Author(s):  
Fusheng Zhao ◽  
Geng Wu ◽  
Yang Wu ◽  
Yunlong Bai ◽  
Yongjia Qiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Endoplasmic Reticulum ◽  
Transcription Factor ◽  
Endoplasmic Reticulum Stress ◽  
Rating Scale ◽  
Motor Dysfunction ◽  
Cord Injury ◽  
Activating Transcription Factor

Abstract Background: Spinal cord injury (SCI) is a severe neurological disorder for which there is currently no effective treatment. Electroacupuncture (EA) is a type of traditional acupuncture combined with modern electrotherapy, which has been widely used and verified to have neuroprotective effects. The aim of this study was to evaluate the effects of EA stimulation on the repair of SCI and to investigate the possible mechanisms. Methods: Sprague-Dawley rats were randomly divided into sham, sham+EA, SCI and SCI+EA four groups. Rat motor function was assessed by the Basso, Beattie and Bresnahan locomotor rating scale, inclined plane test and footprint analysis. Histological alterations of spinal cords were examined with hematoxylin-eosin and Nissl staining. Oxidative stress was evaluated by measuring reactive oxygen species (ROS), glutathione (GSH), total antioxidant capacity (T-AOC), 3-nitrotyrosine (3-NT), and 4-hydroxynonenal (4-HNE) levels. The changes in p66Shc expression and endoplasmic reticulum stress (ERS) were detected to explore the involved mechanisms.Results: EA stimulation significantly improved rat motor functional recovery, reduced spinal cord lesion cavity and neuronal chromatolysis after SCI. Concomitantly, EA stimulation alleviated oxidative stress, as indicated by suppression of ROS production, increase in GSH and T-AOC levels and reduction of 3-NT and 4-HNE expression. Further, EA stimulation markedly eliminated the aberrant increase of p66Shc due to SCI in rats. More notably, EA stimulation was also able to attenuate ERS via down-regulation of glucose-regulated protein 78, activating transcription factor 4, C/EBP homologous protein, X-box binding protein 1 and activating transcription factor 6 expression in rat spinal cord tissues after SCI. Conclusions: These findings suggest that EA is a potential strategy for treatment of SCI, and the mechanism might be, at least in part, associated with mitigation of p66Shc-associated oxidative stress and ERS in rats.

scholarly journals Temporal Profile of Endogenous Anatomical Repair and Functional Recovery following Spinal Cord Injury in Adult Zebrafish

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105857 ◽  
Author(s):  
Katarina Vajn ◽  
Denis Suler ◽  
Jeffery A. Plunkett ◽  
Martin Oudega
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Adult Zebrafish ◽  
Temporal Profile ◽  
Cord Injury

scholarly journals MicroRNA miR-133b is essential for functional recovery after spinal cord injury in adult zebrafish

2011 ◽  
Vol 33 (9) ◽  
pp. 1587-1597 ◽  
Author(s):  
Young-Mi Yu ◽  
Kurt M. Gibbs ◽  
Jonathan Davila ◽  
Neil Campbell ◽  
Simon Sung ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Functional Recovery ◽  
Adult Zebrafish ◽  
Cord Injury

MicroRNA-211-5p attenuates spinal cord injury via targeting of activating transcription factor 6

2021 ◽  
Vol 68 ◽  
pp. 101459
Author(s):  
Haocong Zhang ◽  
Meihui Piao ◽  
Mingming Guo ◽  
Lingzhi Meng ◽  
Hailong Yu
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Transcription Factor ◽  
Cord Injury ◽  
Activating Transcription Factor

scholarly journals The Cell Neural Adhesion Molecule Contactin-2 (TAG-1) Is Beneficial for Functional Recovery after Spinal Cord Injury in Adult Zebrafish

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52376 ◽  
Author(s):  
Jin-Fei Lin ◽  
Hong-Chao Pan ◽  
Li-Ping Ma ◽  
Yan-Qin Shen ◽  
Melitta Schachner
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Adhesion Molecule ◽  
Functional Recovery ◽  
Adult Zebrafish ◽  
Cord Injury ◽  
Neural Adhesion Molecule
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