Blockade of protease-activated receptor 2 attenuates allergen-mediated acute lung inflammation and leukocyte recruitment in mice

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
Natália Alves de Matos ◽  
Onésia Cristina Oliveira Lima ◽  
Josiane Fernandes da Silva ◽  
Annie Rocio Piñeros ◽  
Juliana Carvalho Tavares ◽  
...  
Keyword(s):  
Lung Inflammation ◽  
Leukocyte Recruitment ◽  
Acute Lung Inflammation ◽  
Protease Activated Receptor 2

scholarly journals The metalloproteinase ADAM8 promotes leukocyte recruitment in vitro and in acute lung inflammation

2017 ◽  
Vol 313 (3) ◽  
pp. L602-L614 ◽  
Author(s):  
Daniela Dreymueller ◽  
Jessica Pruessmeyer ◽  
Julian Schumacher ◽  
Sandra Fellendorf ◽  
Franz Martin Hess ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Lung Inflammation ◽  
Leukocyte Recruitment ◽  
Acute Lung Inflammation ◽  
Blood Leukocytes ◽  
Epithelial Layers ◽  
Deficient Mice

Alveolar leukocyte recruitment is a hallmark of acute lung inflammation and involves transmigration of leukocytes through endothelial and epithelial layers. The disintegrin and metalloproteinase (ADAM) 8 is expressed on human isolated leukocytic cells and can be further upregulated on cultured endothelial and epithelial cells by proinflammatory cytokines. By shRNA-mediated knockdown we show that leukocytic ADAM8 is required on monocytic THP-1 cells for chemokine-induced chemotaxis as well as transendothelial and transepithelial migration. Furthermore, ADAM8 promotes αL-integrin upregulation and THP-1 cell adhesion to endothelial cells. On endothelial cells ADAM8 enhances transendothelial migration and increases cytokine-induced permeability. On epithelial cells the protease facilitates migration in a wound closure assay but does not affect transepithelial leukocyte migration. Blood leukocytes and bone marrow-derived macrophages (BMDM) from ADAM8-deficient mice show suppressed chemotactic response. Intranasal application of LPS to mice is accompanied with ADAM8 upregulation in the lung. In this model of acute lung inflammation ADAM8-deficient mice are protected against leukocyte infiltration. Finally, transfer experiments of BMDM in mice indicate that ADAM8 exerts a promigratory function predominantly on leukocytes. Our study provides in vitro and in vivo evidence that ADAM8 on leukocytes holds a proinflammatory function in acute lung inflammation by promoting alveolar leukocyte recruitment.

scholarly journals Protease-Activated Receptor-2 Activation Induces Acute Lung Inflammation by Neuropeptide-Dependent Mechanisms

2005 ◽  
Vol 175 (4) ◽  
pp. 2598-2605 ◽  
Author(s):  
Xiao Su ◽  
Eric Camerer ◽  
Justin R. Hamilton ◽  
Shaun R. Coughlin ◽  
Michael A. Matthay
Keyword(s):  
Lung Inflammation ◽  
Acute Lung Inflammation ◽  
Protease Activated Receptor 2

scholarly journals Combined In Vitro and In Vivo Approaches to Propose a Putative Adverse Outcome Pathway for Acute Lung Inflammation Induced by Nanoparticles: A Study on Carbon Dots

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 180
Author(s):  
Maud Weiss ◽  
Jiahui Fan ◽  
Mickaël Claudel ◽  
Luc Lebeau ◽  
Françoise Pons ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Lung Inflammation ◽  
Adverse Outcome ◽  
Cellular Responses ◽  
Target Cells ◽  
Inflammasome Activation ◽  
Adverse Outcome Pathway ◽  
Acute Lung Inflammation

With the growth of nanotechnologies, concerns raised regarding the potential adverse effects of nanoparticles (NPs), especially on the respiratory tract. Adverse outcome pathways (AOP) have become recently the subject of intensive studies in order to get a better understanding of the mechanisms of NP toxicity, and hence hopefully predict the health risks associated with NP exposure. Herein, we propose a putative AOP for the lung toxicity of NPs using emerging nanomaterials called carbon dots (CDs), and in vivo and in vitro experimental approaches. We first investigated the effect of a single administration of CDs on mouse airways. We showed that CDs induce an acute lung inflammation and identified airway macrophages as target cells of CDs. Then, we studied the cellular responses induced by CDs in an in vitro model of macrophages. We observed that CDs are internalized by these cells (molecular initial event) and induce a series of key events, including loss of lysosomal integrity and mitochondrial disruption (organelle responses), as well as oxidative stress, inflammasome activation, inflammatory cytokine upregulation and macrophage death (cellular responses). All these effects triggering lung inflammation as tissular response may lead to acute lung injury.

Loss of Nucleobindin-2/Nesfatin-1 increases lipopolysaccharide-induced murine acute lung inflammation

2021 ◽  
Author(s):  
Jasmine Hui ◽  
Gurpreet Kaur Aulakh ◽  
Suraj Unniappan ◽  
Baljit Singh
Keyword(s):  
Lung Inflammation ◽  
Acute Lung Inflammation

scholarly journals Blockade of Secondary Lymphoid Tissue Chemokine ExacerbatesPropionibacterium acnes-Induced Acute Lung Inflammation

2001 ◽  
Vol 166 (3) ◽  
pp. 2071-2079 ◽  
Author(s):  
Meiji Itakura ◽  
Atsuko Tokuda ◽  
Hiroshi Kimura ◽  
Shigenori Nagai ◽  
Hiroyuki Yoneyama ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Lung Inflammation ◽  
Acute Lung Inflammation ◽  
Secondary Lymphoid Tissue

scholarly journals Pulmonary Endothelial Protein Kinase C-Delta (PKCδ) Regulates Neutrophil Migration in Acute Lung Inflammation

2014 ◽  
Vol 184 (1) ◽  
pp. 200-213 ◽  
Author(s):  
Mark J. Mondrinos ◽  
Ting Zhang ◽  
Shuang Sun ◽  
Paul A. Kennedy ◽  
Devon J. King ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Lung Inflammation ◽  
Neutrophil Migration ◽  
Acute Lung Inflammation ◽  
Protein Kinase C Delta ◽  
Kinase C

scholarly journals Early Detection of Susceptibility to Acute Lung Inflammation by Molecular Imaging in Mice Exposed to Cigarette Smoke

2011 ◽  
Vol 10 (5) ◽  
pp. 7290.2011.00010 ◽  
Author(s):  
Sandra Pérez-Rial ◽  
Laura del Puerto-Nevado ◽  
Nicolás González-Mangado ◽  
Germán Peces-Barba
Keyword(s):  
Molecular Imaging ◽  
Early Detection ◽  
Cigarette Smoke ◽  
Lung Inflammation ◽  
Acute Lung Inflammation

scholarly journals Induction of acute lung inflammation in mice with hemorrhagic shock and resuscitation: role of HMGB1

2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Raymond LC Kao ◽  
Xuemei Xu ◽  
Anargyros Xenocostas ◽  
Neil Parry ◽  
Tina Mele ◽  
...  
Keyword(s):  
Hemorrhagic Shock ◽  
Lung Inflammation ◽  
Acute Lung Inflammation

Montelukast, Leukotriene Inhibitor, Reduces LPS-Induced Acute Lung Inflammation and Human Neutrophil Activation

2019 ◽  
Vol 55 (11) ◽  
pp. 573-580 ◽  
Author(s):  
Jaime Eduardo Davino-Chiovatto ◽  
Manoel Carneiro Oliveira-Junior ◽  
BreAnne MacKenzie ◽  
Alana Santos-Dias ◽  
Ana Roberta Almeida-Oliveira ◽  
...  
Keyword(s):  
Human Neutrophil ◽  
Lung Inflammation ◽  
Neutrophil Activation ◽  
Acute Lung Inflammation
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