Determination of genetic relationships among elite thermosensitive genic male sterile lines (TGMS) of rice (Oryza sativa L.) employing morphological and simple sequence repeat (SSR) markers

2011 ◽  
Vol 90 (1) ◽  
pp. 11-19 ◽  
Author(s):  
VIKAS KUMAR SINGH ◽  
PRITI UPADHYAY ◽  
PALLAVI SINHA ◽  
ASHISH KUMAR MALL ◽  
SANJAY KUMAR JAISWAL ◽  
...  
2014 ◽  
Vol 8 (2) ◽  
pp. 14-18
Author(s):  
Asmaa K. Aurabi ◽  
Kadhim M. Ibrahim ◽  
Shatha A. Yousif

Simple Sequence Repeat (SSR) markers were used to investigate the variations between drought sensitive and drought tolerant genotypes. Patterns obtained using RM328 and RM302 detected polymorphism between T14, T15 and Amber33, Amber Baghdad genotypes and those obtained using RM316 and RM201 for the tested genotypes suggested that these primers may have the ability to produce drought tolerance markers. According to the patterns obtained using RM189, RM3825 and RM212 primers indicated that these primers cannot be relied on as markers for drought tolerance.


Genome ◽  
2005 ◽  
Vol 48 (5) ◽  
pp. 802-810 ◽  
Author(s):  
Muwang Li ◽  
Li Shen ◽  
Anying Xu ◽  
Xuexia Miao ◽  
Chengxiang Hou ◽  
...  

To determine genetic relationships among strains of silkworm, Bombyx mori L., 31 strains with different origins, number of generations per year, number of molts per generation, and morphological characters were studied using simple sequence repeat (SSR) markers. Twenty-six primer pairs flanking microsatellite sequences in the silkworm genome were assayed. All were polymorphic and unambiguously separated silkworm strains from each other. A total of 188 alleles were detected with a mean value of 7.2 alleles/locus (range 2–17). The average heterozygosity value for each SSR locus ranged from 0 to 0.60, and the highest one was 0.96 (Fl0516 in 4013). The mean polymorphism index content (PIC) was 0.66 (range 0.12–0.89). Unweighted pair group method with arithmetic means (UPGMA) cluster analysis of Nei's genetic distance grouped silkworm strains based on their origin. Seven major ecotypic silkworm groups were analyzed. Principal components analysis (PCA) for SSR data support their UPGMA clustering. The results indicated that SSR markers are an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in the silkworm.Key words: silkworm, Bombyx mori L., microsatellites, simple sequence repeat (SSR), genetic diversity.


2011 ◽  
Vol 59 (2) ◽  
pp. 179-189 ◽  
Author(s):  
S. J. M. Gowda ◽  
G. J. Randhawa ◽  
I. S. Bisht ◽  
P. K. Firke ◽  
A. K. Singh ◽  
...  

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