Germplasm Release: Saikai 35, a Male and Female Fertile Breeding Line Carrying Solanum Phureja-Derived Cytoplasm and Potato Cyst Nematode Resistance (H1) and Potato Virus Y Resistance (Ry chc ) Genes

The tomato Hero A gene is the only member of a multigene family that confers a high level (>80%) of resistance to all the economically important pathotypes of potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida. Although the resistance levels of transgenic tomato lines were similar to those of the tomato line LA1792 containing the introgressed Hero multigene family, transgenic potato plants expressing the tomato Hero A gene are not resistant to PCNs. Comparative microscopy studies of in vitro infected roots of PCN-susceptible tomato cv. Money Maker, the resistant breeding line LA1792, and transgenic line L10 with Ro1 pathotype have revealed no statistically significant difference in the number of juveniles invading roots. However, syncytia (specialized feeding cells) induced in LA1792 and L10 roots mostly were found to have degenerated a few days after their induction, and a few surviving syncytia were able to support only the development of males rather than females. Thus, the ratio between males and females was biased towards males on LA1792 and L10 roots. A series of changes occur in resistant plants leading to formation of a layer of necrotic cells separating the syncytium from stellar conductive tissues and this is followed by degradation of the syncytium. Although the Hero A gene is expressed in all tissues, including roots, stems, leaves, and flower buds, its expression is upregulated in roots in response to PCN infection. Moreover, the expression profiles of the Hero A correlates with the timing of death of the syncytium.

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Comparative sequence analysis of the potato cyst nematode resistance locus H1 reveals a major lack of co-linearity between three haplotypes in potato (Solanum tuberosum ssp.)

Theoretical and Applied Genetics ◽

10.1007/s00122-010-1472-9 ◽

2010 ◽

Vol 122 (3) ◽

pp. 595-608 ◽

Cited By ~ 33

Author(s):

Anna Finkers-Tomczak ◽

Erin Bakker ◽

Jan de Boer ◽

Edwin van der Vossen ◽

Ute Achenbach ◽

...

Keyword(s):

Solanum Tuberosum ◽

Sequence Analysis ◽

Nematode Resistance ◽

Comparative Sequence Analysis ◽

Cyst Nematode ◽

Potato Cyst Nematode ◽

Resistance Locus ◽

Comparative Sequence

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Phenotypic and molecular characterization of potato germplasm for potato cyst nematode resistance

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.79.2.2 ◽

2019 ◽

Vol 79 (02) ◽

Author(s):

R. Sudha ◽

Priyank Hanuman Mhatre ◽

Divya K. Lekshmanan ◽

E. P. Venkatasalam ◽

Aarti Bairwa ◽

...

Keyword(s):

Molecular Characterization ◽

Pollen Viability ◽

Nematode Resistance ◽

Cyst Nematode ◽

Potato Cyst Nematode ◽

Genotypic Resistance ◽

Potato Germplasm ◽

Resistant Varieties ◽

Parental Lines ◽

Breeding Efficiency

Potato cyst nematode (PCN) is considered to be the most devastating pest causing major yield losses in potato worldwide. Resistant potato cultivars would be the most effective strategy to control PCN however, they may not posses enough resistance to all pathotypes of PCN. Therefore breeding programme should focus on developing varieties with horizontal resistance which can be achieved by using parental lines with multiple resistant genes. Hence, the present study was conducted to characterize the phenotypic and genotypic resistance of parental lines against PCN and also an attempt was made to find out the pollen viability of the parental lines to improve the breeding efficiency. Sixty six genotypes were screened phenotypically as well as genotypically against PCN. Out of which, 54 lines were found phenotypically resistant to G. rostochiensis, 48 to G. pallida and 44 to both the species of PCN. The same set of parental lines was also validated using linked molecular markers. In molecular characterization, the markers linked with H1 gene (TG689 and 57R) were found in majority of the parental lines screened. Marker SPUD 1636 linked to GPa5 QTL was positive for three genotypes whereas HC marker (GPaVvrn QTL) was positive for 30 parental lines. TG432 linked to Grp1 gene is positive for 27 parental lines whereas the marker Gro 1-4-1 is not amplified in any of the screened parental lines. Pollen viability of parental lines varied from 15.8 to 93.6%. The results of the study will facilitate breeding of novel resistant varieties by efficient selection of ideal male parent with good pollen potential and multiple resistance to several PCN pathotypes.

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Evaluation and implementation of a potential diagnostic molecular marker for H1-conferred potato cyst nematode resistance in potato (Solanum tuberosum L.)

Plant Breeding ◽

10.1111/j.1439-0523.2012.01949.x ◽

2012 ◽

Vol 131 (2) ◽

pp. 315-321 ◽

Cited By ~ 22

Author(s):

Lee Schultz ◽

Noel O. I. Cogan ◽

Karen McLean ◽

M. Finlay B. Dale ◽

Glenn J. Bryan ◽

...

Keyword(s):

Solanum Tuberosum ◽

Molecular Marker ◽

Solanum Tuberosum L ◽

Nematode Resistance ◽

Cyst Nematode ◽

Potato Cyst Nematode

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Genetic mechanisms of resistance to golden potato cyst nematode Globodera rostochiensis in Solanum phureja

10.18699/bgrssb-2018-130 ◽

2018 ◽

pp. 159-159

Keyword(s):

Globodera Rostochiensis ◽

Cyst Nematode ◽

Potato Cyst Nematode ◽

Solanum Phureja ◽

Mechanisms Of Resistance ◽

Genetic Mechanisms

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Detection of the potato cyst nematode resistance gene Hero A in Japanese tomato cultivars by the PCR-RFLP method

Nematological Research (Japanese Journal of Nematology) ◽

10.3725/jjn.45.115 ◽

2015 ◽

Vol 45 (2) ◽

pp. 115-120 ◽

Cited By ~ 1

Author(s):

Taketo Uehara ◽

Takashi Narabu ◽

Kenji Ito ◽

Chikara Masuta

Keyword(s):

Resistance Gene ◽

Nematode Resistance ◽

Cyst Nematode ◽

Potato Cyst Nematode ◽

Nematode Resistance Gene ◽

Pcr Rflp ◽

Tomato Cultivars

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Potato Virus Y (PVY) y Potato Yellow Vein Virus (PYVV) en Infecciones Mixtas No Causan Síntomas Atípicos en Plantas de Papa

Revista Facultad de Ciencias Básicas ◽

10.18359/rfcb.1297 ◽

2016 ◽

Vol 11 (2) ◽

pp. 26 ◽

Cited By ~ 2

Author(s):

Jenny Paola Alfaro García ◽

Liliana Franco-Lara

Keyword(s):

Solanum Tuberosum ◽

Potato Virus ◽

Potato Virus Y ◽

Yellow Vein ◽

Solanum Phureja ◽

Rt Pcr ◽

Potato Yellow Vein Virus

Observaciones anteriores en cultivos de papa (Solanum tuberosum y Solanum phureja) mostraron síntomas llamados atípicos en este trabajo, que consistían en hojas con manchas irregulares verde oscuro sobre un fondo amarillo intenso, que sugieren la presencia de virus. Estos síntomas no correlacionan con ningún virus descrito para papa en Colombia. En plantas, existen reportes de infecciones virales con dos o más virus que llevan a interacciones como sinergismo o antagonismo. En este trabajo se evaluó la hipótesis de que los síntomas atípicos podrían ser resultado de infecciones mixtas entre PYVV (Potato yellow vein virus) (Crinivirus) y PVY (Potato virus Y) (Potyirus), pues ambos virus son comunes en cultivos de papa en Colombia. Se reportan resultados de RT-PCR (reacción en cadena de la polimerasa mediada por retrotranscripción) para la detección de PYVV y PVY, y de ELISA para PVX, PVS, PVM y PRLV en 57 plantas con y sin síntomas atípicos provenientes de campo. Los resultados no apoyan la hipótesis planteada, pues de 10 plantas con síntomas atípicos evaluadas solo una estaba infectada con los dos virus. Por otro lado, coinfecciones de PVY y PYVV se observaron en plantas sin síntomas aparentes (4 plantas de 5 evaluadas) y en plantas con síntomas característicos de PYVV (17 plantas de 37 evaluadas). Del total de 20 plantas evaluadas por ELISA, 18 presentaban infección por PVX aunque no se observaron síntomas asociados a este virus. Ocho de estas plantas, además de PVX estaban infectadas también con PVY y PYVV, pero mostraban síntomas de característicos de PYVV, lo que sugiere que PVX tampoco correlaciona con los síntomas atípicos. Se sugiere la presencia de un virus no reportado infectando el cultivo de papa en Colombia.

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