scholarly journals A quantitative assay for measuring of bovine immunodeficiency virus using a luciferase-based indicator cell line

2010 ◽  
Vol 25 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Xue Yao ◽  
Hong-yan Guo ◽  
Chang Liu ◽  
Xuan Xu ◽  
Jian-sen Du ◽  
...  
Keyword(s):  
Cell Line ◽  
Quantitative Assay ◽  
Bovine Immunodeficiency Virus ◽  
Indicator Cell Line ◽  
Immunodeficiency Virus ◽  
Indicator Cell

A quantitative assay for measuring human foamy virus using an established indicator cell line

2001 ◽  
Vol 94 (1-2) ◽  
pp. 155-162 ◽  
Author(s):  
Hsiao-Yun Tai ◽  
Kuang-Hui Sun ◽  
Szu-Hao Kung ◽  
Wu-Tse Liu
Keyword(s):  
Cell Line ◽  
Foamy Virus ◽  
Quantitative Assay ◽  
Human Foamy Virus ◽  
Indicator Cell Line ◽  
Indicator Cell

Establishment of an indicator cell line to quantify Bovine Foamy Virus infection

2008 ◽  
Vol 48 (4) ◽  
pp. 278-283 ◽  
Author(s):  
Zhe Ma ◽  
Peng Hao ◽  
Xue Yao ◽  
Chang Liu ◽  
Juan Tan ◽  
...  
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
Foamy Virus ◽  
Bovine Foamy Virus ◽  
Indicator Cell Line ◽  
Indicator Cell

A New Indicator Cell Line to Monitor Human Foamy Virus Infection and Stability in vitro

Intervirology ◽  
2002 ◽  
Vol 45 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Zhi Li ◽  
Ping Yang ◽  
Hui Liu ◽  
Wen-xin Li
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
Foamy Virus ◽  
Human Foamy Virus ◽  
Indicator Cell Line ◽  
Indicator Cell

scholarly journals A novel, sensitive dual-indicator cell line for detection and quantification of inducible, replication-competent latent HIV-1 from reservoir cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fanny Salasc ◽  
David W. Gludish ◽  
Isobel Jarvis ◽  
Saikat Boliar ◽  
Mark R. Wills ◽  
...  
Keyword(s):  
Cell Line ◽  
Parental Cell ◽  
Luciferase Reporter ◽  
Clinical Samples ◽  
Parental Cell Line ◽  
Dependent Manner ◽  
Indicator Cell Line ◽  
Repeated Sampling ◽  
Hiv Research ◽  
Indicator Cell

AbstractUnderstanding the mechanisms involved in HIV infection and latency, and development of a cure, rely on the availability of sensitive research tools such as indicator cells, which allow rigorous quantification of viral activity. Here we describe the construction and validation of a novel dual-indicator cell line, Sup-GGR, which offers two different readouts to quantify viral replication. A construct expressing both Gaussia luciferase and hrGFP in a Tat- and Rev-dependent manner was engineered into SupT1-CCR5 to create Sup-GGR cells. This cell line supports the replication of both X4 and R5-tropic HIV as efficiently as its parental cell line, SupT1-CCR5, and allows repeated sampling without the need to terminate the culture. Sup-GGR demonstrates comparable sensitivity and similar kinetics in virus outgrowth assays (VOA) to SupT1-CCR5 using clinical samples. However the Gaussia luciferase reporter is significantly less labor-intensive and allows earlier detection of reactivated latent viruses compared to the conventional HIV p24 ELISA assay. The Sup-GGR cell line constitutes a versatile new tool for HIV research and clinical trials.

Establishment of a GFP-based indicator cell line to quantitate feline foamy virus

2003 ◽  
Vol 109 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Hang T.T Phung ◽  
Yukinobu Tohya ◽  
Masayuki Shimojima ◽  
Kentaro Kato ◽  
Takayuki Miyazawa ◽  
...  
Keyword(s):  
Cell Line ◽  
Foamy Virus ◽  
Indicator Cell Line ◽  
Indicator Cell

scholarly journals Detection and analysis of bovine foamy virus infection by an indicator cell line

2007 ◽  
Vol 28 (7) ◽  
pp. 994-1000 ◽  
Author(s):  
Zhe MA ◽  
Wen-tao QIAO ◽  
Cheng-hao XUAN ◽  
Jin-hui XIE ◽  
Qi-min CHEN ◽  
...  
Keyword(s):  
Virus Infection ◽  
Cell Line ◽  
Foamy Virus ◽  
Bovine Foamy Virus ◽  
Indicator Cell Line ◽  
Indicator Cell

Rapid phenotypic drug susceptibility assay for HIV-1 with a CCR5 expressing indicator cell line

2000 ◽  
Vol 85 (1-2) ◽  
pp. 151-161 ◽  
Author(s):  
Maria Pirounaki ◽  
Nancy A Vander Heyden ◽  
Max Arens ◽  
Lee Ratner
Keyword(s):  
Cell Line ◽  
Drug Susceptibility ◽  
Indicator Cell Line ◽  
Hiv 1 ◽  
Susceptibility Assay ◽  
Indicator Cell
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