Production of salmosin, a snake venom-derived disintegrin, in recombinant Pichia pastoris using high cell density fed-batch fermentation

2012 ◽  
Vol 17 (5) ◽  
pp. 1068-1075 ◽  
Author(s):  
Myung-Ji Seo ◽  
Hak-Jong Choi ◽  
Kwang-Hoe Chung ◽  
Yu-Ryang Pyun
Keyword(s):  
Pichia Pastoris ◽  
Cell Density ◽  
Snake Venom ◽  
Batch Fermentation ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Recombinant Pichia Pastoris ◽  
Fed Batch Fermentation

scholarly journals High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

2010 ◽  
Vol 76 (23) ◽  
pp. 7890-7895 ◽  
Author(s):  
Mohammad H. A. Ibrahim ◽  
Alexander Steinb�chel
Keyword(s):  
Cell Density ◽  
Batch Fermentation ◽  
High Cell Density ◽  
Dry Weight ◽  
Thermophilic Bacterium ◽  
High Cell ◽  
Fed Batch ◽  
G Cell ◽  
Fed Batch Fermentation

ABSTRACT Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 � 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 � 4.3 g cell dry weight/liter was achieved.

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