Inhibitory effects of chlorogenic acid on aldose reductase activity in vitro and cataractogenesis in galactose-fed rats

Oxidative stress and aldose reductase activity have been implicated in the development of diabetic complications. In this study, the antioxidant and aldose reductase (AR) inhibitory effects ofMaackia amurensis(MA) were investigated. The ethyl acetate fraction of the MA extract showed the highest inhibitory activity in antioxidant and rat lens AR (RLAR). To identify and isolate the active components in the ethyl acetate fraction of the MA extract, high-speed countercurrent chromatography and Sephadex LH-20 column chromatography were performed and guided by an offline HPLC-ABTS assay and HPLC microfractionation AR assay. Four antioxidants, namely, piceatannol (IC50= 6.73 μM), resveratrol (IC50= 11.05 μM),trans-ferulic acid (IC50= 13.51 μM), and chlorogenic acid (IC50= 27.23 μM), and six AR inhibitors, namely, chlorogenic acid (IC50= 4.2 μM), tectoridin (IC50= 50.4 μM), genistein (IC50= 57.1 μM), formononetin (IC50= 69.2 μM), resveratrol (IC50= 117.6 μM), and daidzein (IC50= 151.9 μM), were isolated and identified. The screening results of the offline HPLC-ABTS assay and HPLC microfractionation AR assay matched the activity of isolated compounds. Thus, MA is potentially valuable for antioxidant and AR inhibitor discovery and efficient drug design for the prevention and treatment of diabetic complications.

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The Inhibitory Effect ofPrunella vulgarisL. on Aldose Reductase and Protein Glycation

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/928159 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Hong Mei Li ◽

Jin Kyu Kim ◽

Jai Man Jang ◽

Sang Oh Kwon ◽

Cheng Bi Cui ◽

...

Keyword(s):

Aldose Reductase ◽

Inhibitory Effect ◽

Protein Glycation ◽

Prunella Vulgaris ◽

Inhibitory Effects ◽

Advanced Glycation ◽

High Concentration ◽

Potent Inhibition ◽

Glycation End Products

To evaluate the aldose reductase (AR) enzyme inhibitory ability ofPrunella vulgarisL. extract, six compounds were isolated and tested for their effects. The components were subjected toin vitrobioassays to investigate their inhibitory assays using rat lens aldose reductase (rAR) and human recombinant AR (rhAR). Among them, caffeic acid ethylene ester showed the potent inhibition, with the IC50values of rAR and rhAR at3.2±0.55 μM and12.58±0.32 μM, respectively. In the kinetic analyses using Lineweaver-Burk plots of 1/velocity and 1/concentration of substrate, this compound showed noncompetitive inhibition against rhAR. Furthermore, it inhibited galactitol formation in a rat lens incubated with a high concentration of galactose. Also it has antioxidative as well as advanced glycation end products (AGEs) inhibitory effects. As a result, this compound could be offered as a leading compound for further study as a new natural products drug for diabetic complications.

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Esculetin, a Coumarin Derivative, Inhibits Aldose Reductase Activity in vitro and Cataractogenesis in Galactose-Fed Rats

Biomolecules & Therapeutics ◽

10.4062/biomolther.2015.101 ◽

2016 ◽

Vol 24 (2) ◽

pp. 178-183 ◽

Cited By ~ 13

Author(s):

Chan-Sik Kim ◽

Junghyun Kim ◽

Yun Mi Lee ◽

Eunjin Sohn ◽

Jin Sook Kim

Keyword(s):

Aldose Reductase ◽

Reductase Activity ◽

Coumarin Derivative

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Screening inhibitory effects of selected flavonoids on human recombinant aldose reductase enzyme: in vitro and in silico study

Archives of Physiology and Biochemistry ◽

10.1080/13813455.2020.1771377 ◽

2020 ◽

pp. 1-7

Author(s):

Veysel Comakli ◽

Sevki Adem ◽

Aykut Oztekin ◽

Ramazan Demirdag

Keyword(s):

Aldose Reductase ◽

In Silico ◽

Inhibitory Effects ◽

In Silico Study

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Deferoxamine: a reversible S-phase inhibitor of human lymphocyte proliferation

Blood ◽

10.1182/blood.v64.3.748.bloodjournal643748 ◽

1984 ◽

Vol 64 (3) ◽

pp. 748-753 ◽

Cited By ~ 3

Author(s):

HM Lederman ◽

A Cohen ◽

JW Lee ◽

MH Freedman ◽

EW Gelfand

Keyword(s):

Ribonucleotide Reductase ◽

Lymphocyte Proliferation ◽

Potent Inhibitor ◽

Reductase Activity ◽

S Phase ◽

Ferric Ion ◽

Inhibitory Effects ◽

Ribonucleotide Reductase Activity ◽

B And T Lymphocytes

Deferoxamine is widely used therapeutically as a chelator of ferric ion in disorders of iron overload. This study demonstrates that this drug is a potent inhibitor of DNA synthesis by human B and T lymphocytes in vitro, but has relatively little effect on the synthesis of RNA and protein. The inhibitory effects of deferoxamine are completely reversible by washing or by adding stoichiometric amounts of Fe3+. Micromolar concentrations of deferoxamine decrease intracellular levels of deoxyribonucleoside triphosphates, which is similar to the effects of hydroxyurea. The binding of iron by deferoxamine likely causes an inhibition of ribonucleotide reductase activity, thereby preventing cells from completing the S phase of the cell proliferation cycle. As a reversible and nontoxic S-phase inhibitor, it may have important experimental and therapeutic applications.

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In Vitro Inhibitory Effects of Moringa oleifera Leaf Extract and Its Major Components on Chemiluminescence and Chemotactic Activity of Phagocytes

Natural Product Communications ◽

10.1177/1934578x1300801115 ◽

2013 ◽

Vol 8 (11) ◽

pp. 1934578X1300801 ◽

Cited By ~ 5

Author(s):

Boonyadist Vongsak ◽

Wandee Gritsanapan ◽

Yuvadee Wongkrajang ◽

Ibrahim Jantan

Keyword(s):

Chlorogenic Acid ◽

Oxidative Burst ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Boyden Chamber ◽

Inhibitory Effects ◽

Chemotactic Migration ◽

Human Polymorphonuclear Leukocytes ◽

Moringa Oleifera Lam

The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3- O-glucoside and kaempferol 3- O-glucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3- O-glucoside, crypto-chlorogenic acid, and kaempferol 3- O-glucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 μM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 μM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.

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