Anti-inflammatory activity of 6-hydroxy-2,7-dimethoxy-1,4-henanthraquinone from tuberous roots of Yam (Dioscorea batatas) through inhibition of prostaglandin D2 and leukotriene C4 production in mouse bone marrow-derived mast cells

2011 ◽  
Vol 34 (9) ◽  
pp. 1495-1501 ◽  
Author(s):  
Meihua Jin ◽  
Yue Lu ◽  
Ju Hye Yang ◽  
Tae Hyung Jo ◽  
Young In Park ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Inflammatory Activity ◽  
Prostaglandin D2 ◽  
Mouse Bone Marrow ◽  
Leukotriene C4 ◽  
Tuberous Roots ◽  
Anti Inflammatory ◽  
Dioscorea Batatas ◽  
C4 Production

scholarly journals IgE-mediated release of leukotriene C4, chondroitin sulfate E proteoglycan, beta-hexosaminidase, and histamine from cultured bone marrow-derived mouse mast cells.

1983 ◽  
Vol 157 (1) ◽  
pp. 189-201 ◽  
Author(s):  
E Razin ◽  
J M Mencia-Huerta ◽  
R L Stevens ◽  
R A Lewis ◽  
F T Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Mast Cells ◽  
Chondroitin Sulfate ◽  
Specific Antigen ◽  
Antigen Challenge ◽  
Prostaglandin D2 ◽  
Mouse Bone Marrow ◽  
Leukotriene C4 ◽  
Chondroitin Sulfate E

Mouse bone marrow-derived mast cells differentiated in vitro and sensitized with monoclonal IgE respond to antigen-initiated activation with the release of histamine, beta-hexosaminidase, chondroitin sulfate E proteoglycan, and leukotriene C4 (LTC4). The chondroitin sulfate E nature of the glycosaminoglycan side chain was established by demonstrating that the chondroitinase ABC disaccharide digestion products were composed of equal quantities of 4-sulfated and 4,6-disulfated N-acetyl-galactosamine. The single immunoreactive sulfidopeptide leukotriene, released and quantitated with a class-specific antibody, was identified as LTC4 by its retention time on reverse-phase high-performance liquid chromatography and by its specific spasmogenic activity on the guinea pig ileum. The release of the preformed mediators, as well as of LTC4, was related in a dose-response fashion to the concentration of monoclonal IgE used during the sensitization step and to the concentration of specific antigen used to initiate the activation-secretion response. The optimal concentrations of IgE for sensitization and of antigen for challenge were the same for the release of preformed mediators and of LTC4. In addition, the time courses of their release were superimposable, with a plateau at 5 min after antigen challenge. The release of three preformed mediators and of LTC4 after fixation of IgE, washing of the sensitized cells, and antigen challenge unequivocally indicates a bone marrow-derived mast cell origin for these products. Linear regression analyses of the net percent release of beta-hexosaminidase to histamine and of 35S-chondroitin sulfate E to beta-hexosaminidase yielded straight lines that intersected at the origin, which indicates that the three preformed mediators are localized in the secretory granules of the bone marrow-derived mast cells. The concomitant generation of 23 ng of LTC4/10(6) sensitized bone marrow-derived mast cells represents the first example of IgE-dependent release of substantial amounts of LTC4, a component of slow reacting substance of anaphylaxis, from a mast cell population of greater than 95% purity. The IgE-dependent generation of LTC4, rather than prostaglandin D2, by the chondroitin sulfate E proteoglycan-containing bone marrow-derived mast cells contrasts with the predominant generation of prostaglandin D2 by heparin proteoglycan-containing mast cells. These differences together support the existence of two phenotypically different mast cell subclasses.

Anti-Inflammatory and Anti-Allergic Effect of Agaricus blazei Extract in Bone Marrow-Derived Mast Cells

2012 ◽  
Vol 40 (05) ◽  
pp. 1073-1084 ◽  
Author(s):  
Hyuk-Hwan Song ◽  
Hee-Sung Chae ◽  
Sei-Ryang Oh ◽  
Hyeong-Kyu Lee ◽  
Young-Won Chin
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Mouse Bone Marrow ◽  
Agaricus Blazei ◽  
Soluble Extract ◽  
Anti Inflammatory ◽  
Allergic Effect

In this study, the anti-inflammatory and anti-allergic effects of the chloroform-soluble extract of Agaricus blazei in mouse bone marrow-derived mast cells (BMMCs) were investigated. The chloroform-soluble extract inhibited IL-6 production in PMA plus A23187-stimulated BMMCs, and down-regulated the phosphorylation of Akt. In addition, this extract demonstrated inhibition of the degranulation of β-hexosaminidase and the production of IL-6, prostaglandin D2 and leukotriene C4 in PMA plus A23187-induced BMMCs. In conclusion, the chloroform-soluble extract of Agaricus blazei exerted anti-inflammatory and anti-allergic activities mediated by influencing IL-6, prostaglandin D2, leukotriene C4, and the phosphorylation of Akt.

scholarly journals Generation of leukotriene C4 from a subclass of mast cells differentiated in vitro from mouse bone marrow.

1982 ◽  
Vol 79 (15) ◽  
pp. 4665-4667 ◽  
Author(s):  
E. Razin ◽  
J. M. Mencia-Huerta ◽  
R. A. Lewis ◽  
E. J. Corey ◽  
K. F. Austen
Keyword(s):  
Bone Marrow ◽  
Mast Cells ◽  
Mouse Bone Marrow ◽  
Leukotriene C4
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