MALDI-TOF MS: Foundations and a Practical Approach to the Clinically Relevant Filamentous Fungi Identification

2021 ◽  
Author(s):  
Juan C. Gómez-Velásquez ◽  
Iván L. Mojica-Figueroa ◽  
Cledir Santos ◽  
Nelson Lima ◽  
Ana C. Mesa-Arango
Keyword(s):  
Filamentous Fungi ◽  
Practical Approach ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper

2020 ◽  
Vol 58 (5) ◽  
Author(s):  
Marie Gladys Robert ◽  
Charlotte Romero ◽  
Céline Dard ◽  
Cécile Garnaud ◽  
Odile Cognet ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Extraction Protocol ◽  
Maldi Tof ◽  
Morphological Aspects ◽  
Routine Identification ◽  
Tof Ms

ABSTRACT MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol. Antifungal susceptibility testing and microscopic morphology after subculture on SAB and IDFP were also compared for ten molds. Growth rates and morphological aspects were similar for the three media. With IDFP, harvesting of fungal material for the extraction procedure was rapid and easy in 92.4% of cases, whereas it was tedious on SAB or CAN2 in 65.2% and 80.3% of cases, respectively. The proportion of scores above 1.7 (defined as acceptable identification) were comparable for both extraction protocols using IDFP (P = 0.256). Moreover, rates of acceptable identification after DT performed on IDFP (93.9%) were significantly higher than those obtained after EA extraction with SAB (69.7%) or CAN2 (71.2%) (P = <0.001 and P = 0.001, respectively). Morphological aspects and antifungal susceptibility testing were similar between IDFP and SAB. IDFP is a culture plate that facilitates and improves the identification of filamentous fungi, allowing accurate routine identification of molds with MALDI-TOF-MS using a rapid-extraction protocol.

scholarly journals Evaluation of the new Id‐Fungi plates from Conidia for MALDI‐TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Mycoses ◽  
2020 ◽  
Vol 63 (10) ◽  
pp. 1115-1127 ◽  
Author(s):  
Rosalie Sacheli ◽  
Anne‐Sophie Henri ◽  
Laurence Seidel ◽  
Marie Ernst ◽  
Rajae Darfouf ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Conventional Methods ◽  
Skin Samples ◽  
Tof Ms ◽  
Identification Tool

scholarly journals Assessment of various parameters to improve MALDI-TOF MS reference spectra libraries constructed for the routine identification of filamentous fungi

2013 ◽  
Vol 13 (1) ◽  
pp. 76 ◽  
Author(s):  
Anne-Cécile Normand ◽  
Carole Cassagne ◽  
Stéphane Ranque ◽  
Coralie L’Ollivier ◽  
Patrick Fourquet ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Routine Identification ◽  
Tof Ms

Multicenter evaluation of three different MALDI-TOF MS systems for identification of clinically relevant filamentous fungi

2020 ◽  
Vol 59 (1) ◽  
pp. 81-86
Author(s):  
Yue Sun ◽  
Jian Guo ◽  
Rong Chen ◽  
Liang Hu ◽  
Qihang Xia ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Filamentous Fungi ◽  
Reference Method ◽  
Species Level ◽  
Its Sequencing ◽  
Maldi Tof Ms ◽  
Genus Level ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Abstract Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) holds promise as a potential tool for clinical identification of filamentous fungi. However, due to the lack of an appropriate extraction protocol and the difficulty of database building, the identification power of each system differs. In this study, we selected 126 clinical mould isolates comprising 28 species identified using internal transcribed spacer (ITS) sequencing as the reference method to evaluate three MALDI-TOF MS systems. When using cultures and sample preparation as recommended by the respective vendors, of the 126 strains tested, VITEK MS identified 121 (96.0%) to species-level and 124 (98.4%) to genus-level; Biotyper identified 53 (42.1%) to species-level and 54 (42.9%) to genus-level; Autof identified 74 (58.7%) to species-level and 76 (60.3%) to genus-level. For the Autof system, the tube extraction method recommended by the vendor performed better (59%) than the on-plate lysis (51%). Our study demonstrates that MALDI-TOF MS systems can successfully identify most clinically relevant fungi, while performance is still highly dependent on the database and sample preparation protocol.

scholarly journals Increased species-assignment of filamentous fungi using MALDI-TOF MS coupled with a simplified sample processing and an in-house library

2018 ◽  
Vol 57 (1) ◽  
pp. 63-70 ◽  
Author(s):  
M E Zvezdanova ◽  
P Escribano ◽  
A Ruiz ◽  
M C Martínez-Jiménez ◽  
T Peláez ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Sample Processing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Species Assignment ◽  
Tof Ms

scholarly journals Species Identification and Delineation of Pathogenic Mucorales by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2018 ◽  
Vol 56 (4) ◽  
pp. e01886-17 ◽  
Author(s):  
Jin Shao ◽  
Zhe Wan ◽  
Ruoyu Li ◽  
Jin Yu
Keyword(s):  
Filamentous Fungi ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Reference Strains ◽  
Tof Ms

ABSTRACT This study aimed to validate the effectiveness of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)-based identification of filamentous fungi of the order Mucorales. A total of 111 isolates covering six genera preserved at the Research Center for Medical Mycology of Peking University were selected for MALDI-TOF MS analysis. We emphasized the study of 23 strains of Mucor irregularis predominantly isolated from patients in China. We first used the Bruker Filamentous Fungi library (v1.0) to identify all 111 isolates. To increase the identification rate, we created a compensatory in-house database, the Beijing Medical University (BMU) database, using 13 reference strains covering 6 species, including M. irregularis, Mucor hiemalis, Mucor racemosus, Cunninghamella bertholletiae, Cunninghamella phaeospora, and Cunninghamella echinulata. All 111 isolates were then identified by MALDI-TOF MS using a combination of the Bruker library and BMU database. MALDI-TOF MS identified 55 (49.5%) and 74 (66.7%) isolates at the species and genus levels, respectively, using the Bruker Filamentous Fungi library v1.0 alone. A combination of the Bruker library and BMU database allowed MALDI-TOF MS to identify 90 (81.1%) and 111 (100%) isolates at the species and genus levels, respectively, with a significantly increased accuracy rate. MALDI-TOF MS poorly identified Mucorales when the Bruker library was used alone due to its lack of some fungal species. In contrast, this technique perfectly identified M. irregularis after main spectrum profiles (MSPs) of relevant reference strains were added to the Bruker library. With an expanded Bruker library, MALDI-TOF MS is an effective tool for the identification of pathogenic Mucorales.

Capillary and gel electromigration techniques and MALDI-TOF MS – Suitable tools for identification of filamentous fungi

2012 ◽  
Vol 716 ◽  
pp. 155-162 ◽  
Author(s):  
Marie Horká ◽  
Anna Kubesová ◽  
Jiří Šalplachta ◽  
Eva Zapletalová ◽  
Jaroslav Horký ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification

2019 ◽  
Vol 5 (1) ◽  
pp. 4 ◽  
Author(s):  
Robin Patel
Keyword(s):  
Mass Spectrometry ◽  
Filamentous Fungi ◽  
Bacterial Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Fungal Identification ◽  
Routine Identification ◽  
Tof Ms

As a result of its being inexpensive, easy to perform, fast and accurate, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) is quickly becoming the standard means of bacterial identification from cultures in clinical microbiology laboratories. Its adoption for routine identification of yeasts and even dimorphic and filamentous fungi in cultures, while slower, is now being realized, with many of the same benefits as have been recognized on the bacterial side. In this review, the use of MALDI-ToF MS for identification of yeasts, and dimorphic and filamentous fungi grown in culture will be reviewed, with strengths and limitations addressed.

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