scholarly journals Assessing the Extent of Substitution Rate Variation of Retrotransposon Long Terminal Repeat Sequences in Oryza sativa and Oryza glaberrima

Rice ◽  
2010 ◽  
Vol 3 (4) ◽  
pp. 242-250 ◽  
Author(s):  
Andrea Zuccolo ◽  
Aswathy Sebastian ◽  
Yeisoo Yu ◽  
Scott Jackson ◽  
Steve Rounsley ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Long Terminal Repeat ◽  
Substitution Rate ◽  
Terminal Repeat ◽  
Rate Variation ◽  
Oryza Glaberrima ◽  
Repeat Sequences ◽  
Substitution Rate Variation

Human retroviral sequences on the Y chromosome

1987 ◽  
Vol 7 (4) ◽  
pp. 1559-1562
Author(s):  
J Silver ◽  
A Rabson ◽  
T Bryan ◽  
R Willey ◽  
M A Martin
Keyword(s):  
Gene Duplication ◽  
Long Terminal Repeat ◽  
Y Chromosome ◽  
Full Length ◽  
Terminal Repeat ◽  
Male And Female ◽  
Southern Blots ◽  
Repeat Sequences ◽  
Flanking Regions ◽  
Human Retrovirus

Novel endogenous human retroviral sequences were cloned by low-stringency hybridization, using the pol gene of endogenous human retrovirus 51-1. One clone, lambda NP-2, contained gag, pol, env, and long terminal repeat sequences related to the corresponding portions of clone 51-1 and the closely related full-length endogenous human retrovirus 4-1. The sequence of the env gene of NP-2 was 73% homologous to that of 4-1. Genomic Southern blots of male and female DNAs showed that NP-2 is located on the Y chromosome and that the Y chromosome also contains one other sequence closely related to the env and 3' flanking regions of NP-2. Conservation of flanking DNA suggests that the second Y chromosome copy of the NP-2 env sequence arose by gene duplication rather than provirus insertion.

scholarly journals Human retroviral sequences on the Y chromosome.

1987 ◽  
Vol 7 (4) ◽  
pp. 1559-1562 ◽  
Author(s):  
J Silver ◽  
A Rabson ◽  
T Bryan ◽  
R Willey ◽  
M A Martin
Keyword(s):  
Gene Duplication ◽  
Long Terminal Repeat ◽  
Y Chromosome ◽  
Full Length ◽  
Terminal Repeat ◽  
Male And Female ◽  
Southern Blots ◽  
Repeat Sequences ◽  
Flanking Regions ◽  
Human Retrovirus

Novel endogenous human retroviral sequences were cloned by low-stringency hybridization, using the pol gene of endogenous human retrovirus 51-1. One clone, lambda NP-2, contained gag, pol, env, and long terminal repeat sequences related to the corresponding portions of clone 51-1 and the closely related full-length endogenous human retrovirus 4-1. The sequence of the env gene of NP-2 was 73% homologous to that of 4-1. Genomic Southern blots of male and female DNAs showed that NP-2 is located on the Y chromosome and that the Y chromosome also contains one other sequence closely related to the env and 3' flanking regions of NP-2. Conservation of flanking DNA suggests that the second Y chromosome copy of the NP-2 env sequence arose by gene duplication rather than provirus insertion.

Viral Substitution Rate Variation Can Arise from the Interplay between Within-Host and Epidemiological Dynamics

2013 ◽  
Vol 182 (4) ◽  
pp. 494-513 ◽  
Author(s):  
Stacy O. Scholle ◽  
Rolf J. F. Ypma ◽  
Alun L. Lloyd ◽  
Katia Koelle
Keyword(s):  
Substitution Rate ◽  
Rate Variation ◽  
Substitution Rate Variation

Mapping of a major osteomagenic determinant of murine leukemia virus RFB-14 to non-long terminal repeat sequences.

1997 ◽  
Vol 71 (1) ◽  
pp. 645-649 ◽  
Author(s):  
M Ostergaard ◽  
L Pedersen ◽  
J Schmidt ◽  
A Luz ◽  
J Lovmand ◽  
...  
Keyword(s):  
Long Terminal Repeat ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Terminal Repeat ◽  
Repeat Sequences ◽  
Murine Leukemia

scholarly journals Differences in intracellular DNA ligation after microinjection and transfection.

1984 ◽  
Vol 4 (2) ◽  
pp. 240-246 ◽  
Author(s):  
J J Kopchick ◽  
D W Stacey
Keyword(s):  
Long Terminal Repeat ◽  
Plasmid Dna ◽  
Terminal Repeat ◽  
Viral Rna ◽  
Dna Molecule ◽  
Repeat Sequences ◽  
Dna Ligation ◽  
Avian Cell ◽  
Pbr322 Plasmid ◽  
Avian Sarcoma

An uninterrupted avian sarcoma viral genome terminated by viral long terminal repeat sequences was cloned into a pBR322 plasmid. After introduction into a cultured avian cell, transcription of either the circular plasmid molecule or one linearized within the pBR322 sequences could initiate and terminate at long terminal repeat sequences, yielding full-sized viral RNA. A plasmid DNA molecule linearized by cleavage within the viral pol gene, on the other hand, would have to undergo ligation to yield full-sized viral RNA. Microinjection of each of these three types of DNA into the nuclei of quail cells promoted the release of similar virus titers, indicating that the plasmid DNA cleaved within the viral pol gene had been efficiently and accurately ligated. When plasmid DNA was transfected into quail cells, circular and pBR322-cleaved molecules directed the synthesis of similar virus titers, indicating that they were similarly taken up and utilized by the cells. Compared with these results, plasmid DNA cleaved within the pol gene was reduced in activity over 95% after transfection. This reduction did not result from inefficient ligation but from the generation of mutations (of limited size) during ligation of the transfected molecules. Mutations were not observed after microinjection even into the cytoplasm. Consistent with these findings, transfected DNA termini were found to be joined regardless of their structure, whereas ligation after microinjection required that single-stranded protruding DNA termini be complementary.

Sign in / Sign up

Export Citation Format

Share Document