Validation of SSR markers associated with rust (Uromyces fabae) resistance in pea (Pisum sativum L.)

AbstractThe aim of this study was to identify the molecular and morphological characteristics of Turkish pea accessions (Pisum sativum L.). The genetic diversity among 130 Turkish landraces and 2 commercial varieties in a total of 132 pea accessions was assessed with 14 simple sequence repeat (SSR) markers. Forty-eight (48) polymorphic alleles were identified using 14 SSR markers. The pairwise Dice coefficients of similarity between accessions ranged from 0.091 to 0.960. The polymorphism information content (PIC) value ranged from 0.585 to 0.861. Overall, 50 morphological traits were evaluated. Cluster analysis was carried out on a matrix of Euclidean distances. The accessions were divided into three main groups. Principal component analysis (PCA) was used to identify the weight of each morphological characteristic. According to the results, the highest eigenvalue was observed in PC-I (13.88) followed by PC-II (11.42), and PC-III (7.32). The first fifteen PCs with eigenvalues > 1 explained 74.08% of the variability. The results showed that the molecular markers were useful and polymorphic, sufficient to allocate all the evaluated accessions. This research has provided significant insights into the genetic variability of Turkish pea accessions.

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Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03871-6 ◽

2021 ◽

Author(s):

Longfei Wu ◽

Rudolph Fredua-Agyeman ◽

Sheau-Fang Hwang ◽

Kan-Fa Chang ◽

Robert L. Conner ◽

...

Keyword(s):

Pisum Sativum ◽

Ssr Markers ◽

Root Rot ◽

Partial Resistance ◽

Snp Array ◽

Pisum Sativum L ◽

Mapping Qtl

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Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia

Revista Colombiana de Ciencias Hortícolas ◽

10.17584/rcch.2019v13i2.10177 ◽

2019 ◽

Vol 13 (2) ◽

pp. 208-218

Author(s):

Juan Diego Duque-Zapata ◽

Jaime Eduardo Muñoz ◽

Oscar Checa-Coral

Keyword(s):

Pisum Sativum ◽

Ssr Markers ◽

Polymorphic Information Content ◽

Genetic Relationships ◽

Similarity Index ◽

Morphological Characteristics ◽

High Similarity ◽

Expected Heterozygosity ◽

Pisum Sativum L ◽

Simple Sequence

The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.

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