Artificial Inoculation Method of Pokkah Boeng Disease of Sugarcane and Screening of Resistant Germplasm Resources in Subtropical China

Sugar Tech ◽  
2016 ◽  
Vol 19 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Ze-Ping Wang ◽  
Hai-Jun Sun ◽  
Qiang Guo ◽  
Shi-Qiang Xu ◽  
Ji-Hua Wang ◽  
...  
Keyword(s):  
Artificial Inoculation ◽  
Germplasm Resources ◽  
Subtropical China ◽  
Inoculation Method ◽  
Pokkah Boeng

scholarly journals Metode inokulasi buatan untuk menguji infeksi Peronosclerospora maydis penyebab penyakit bulai tanaman jagung

Jurnal Agro ◽  
10.15575/4409 ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 77-85
Author(s):  
Satriyo Restu Adhi ◽  
Fitri Widiantini ◽  
Endah Yulia
Keyword(s):  
Downy Mildew ◽  
Artificial Inoculation ◽  
Resistance Testing ◽  
Infected Leaf ◽  
Suspension Spraying ◽  
Plant Materials ◽  
Inoculation Method ◽  
Resistant Varieties ◽  
Maize Plants ◽  
Leaf Insertion

Keberadaan penyakit bulai akibat Peronosclerospora maydis pada tanaman jagung mampu menyebabkan kehilangan hasil hingga 100%. Pengendalian penyakit bulai dengan menggunakan varietas tahan dinilai cukup efektif. Di dalam pengembangan varietas tahan, seleksi ketahanan calon varietas baru harus dilakukan dan hal ini akan melibatkan inokulasi buatan. Tujuan dari penelitian ini adalah untuk mendapatkan metode inokulasi buatan Peronosclerospora maydis yang efektif pada tanaman jagung pada skala rumah kaca. Penelitian dilaksanakan di Laboratorium Bioteknologi Proteksi Tanaman dan rumah kaca Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran pada bulan Oktober hingga Desember 2018. Bahan tanaman yang diinokulasikan berupa kecambah dan bibit tanaman jagung. Metode inokulasi buatan yang diujikan terdiri atas: (1) Sisip daun terinfeksi bulai pada kecambah (SD), (2) Semprot suspensi konidia pada kecambah (SI), (3) Sisip daun bergejala bulai + semprot suspensi konidia pada kecambah (SS), (4) Rendam kecambah pada suspensi konidia (RS), (5) Semprot suspensi konidia pada bibit (SB), dan dua perlakuan tanpa inokulasi (kontrol). Dari hasil percobaan metode sisip daun (SD) menyebabkan 83,3% tanaman jagung terinfeksi bulai dan dinilai sebagai metode inokulasi buatan yang efisien.Kata kunci: bulai, jagung, inokulasi buatan, Peronosclerospora maydis. ABSTRACTDowny mildew disease caused by Peronosclerospora maydis in maize plants can cause yield loss up to 100%. Disease management of downy mildew by using resistant varieties is considered quite effective. One of the steps to develop resistant varieties is resistance testing of the new variety candidates that involve artificial inoculation. The purpose of this study was to obtain an effective artificial inoculation method of Peronosclerospora maydis  in maize plants in greenhouse. The research was conducted at the Laboratory of Biotechnology of Plant Protection and Glasshouse Department of Plant Pests and Diseases, Agriculture Faculty, Universitas Padjadjaran from October to December 2018. The plant materials used for inoculation were sprout and seedling of maize plant. The artificial inoculation methods tested were (1) infected leaf insertion among the sprouts (SD), (2) conidia suspension spraying on the sprouts (SI), (3) infected leaf insertion + conidia suspension spraying on the sprouts (SS), (4) sprout soaking in conidia suspension (RS), (5) conidia suspension spraying on the seedling (SB), and two treatments without inoculation (controls). The result showed that the insertion of infected leaves among the sprouts (SD) treatment resulted in 83.3% of infected maize plants by the downy mildew and was considered as the most efficient inoculation method. 

scholarly journals An Artificial Inoculation Method to Select Mature Onion Bulbs Resistant to Fusarium Basal Rot

HortScience ◽  
2020 ◽  
Vol 55 (11) ◽  
pp. 1840-1847
Author(s):  
Subhankar Mandal ◽  
Christopher S. Cramer
Keyword(s):  
Basal Plate ◽  
Artificial Inoculation ◽  
Economic Damage ◽  
Screening Methods ◽  
Field Inoculation ◽  
Inoculation Method ◽  
Short Day ◽  
Onion Bulbs ◽  
Basal Rot ◽  
Fusarium Basal Rot

Fusarium basal rot (FBR) of onion, which is caused by Fusarium oxysporum f.sp. cepae (Hanzawa) Snyder & Hansen (FOC) results in a substantial loss of marketable bulbs worldwide. One of the main reasons for the lack of FBR-resistant short-day cultivars is the unreliable screening methods available for the mature bulb stage when significant economic damage occurs. The objective of this study was to develop an artificial inoculation method with better quantification of inoculum for an effective selection of FBR-resistant mature onion bulbs. Mature bulbs of seven New Mexican short-day onion cultivars, along with susceptible and tolerant controls, were selected and evaluated for FBR resistance using mycelial and conidial inoculation methods, respectively. Transversely cut basal plates of mature bulbs were inoculated artificially with mycelia or conidia (12 × 105 spores/mL in 2014 and 3 × 105 spores/mL in 2015 embedded in potato dextrose agar plug) of a virulent FOC isolate ‘CSC-515’. Mature bulb evaluation using a visual rating scale (1 = no disease; 9 = >70% basal plate infected) revealed a high degree of FBR severity and incidence irrespective of the genetic background of the cultivars, minimizing the chance of disease escape, which is a significant problem in field inoculation. An attempt to inoculate intact basal plates postharvest resulted in minimal disease development, suggesting that mechanical resistance was conferred by the dry outer layer of the basal plate. The high selection pressure conferred by the conidial inoculation method developed in this study can effectively screen FBR-resistant onion bulbs to replace an unreliable field screening. Concentrations of the conidia lower than 3 × 105 spores/mL are recommended to detect subtle genetic differences in FBR resistance among the onion cultivars and their selected population.

Establishment of an Artificial Inoculation Method of Ustilaginoidea virens without Damaging the Rice Panicle Sheaths

Plant Disease ◽  
2021 ◽  
Author(s):  
Xianfeng Hu ◽  
Jian Wang ◽  
Rongyu Li ◽  
Xiaomao Wu ◽  
Xiubing Gao ◽  
...  
Keyword(s):  
Natural Infection ◽  
Infection Process ◽  
Artificial Inoculation ◽  
Flowering Stage ◽  
Ustilaginoidea Virens ◽  
Inoculation Method ◽  
Young Panicle ◽  
False Smut ◽  
The Mean ◽  
Sheath Structure

Rice false smut (RFS) is a destructive disease of rice worldwide caused by Ustilaginoidea virens. There is a lack of efficient and stable artificial inoculation method to simulate the natural infection of U. virens, which is an important factor limiting further research on the disease. The purpose of this study was to establish an artificial inoculation method, which can simulate the natural infection process of U. virens without destroying the panicle sheath structure of rice. In this research, rice plants were inoculated by soaking roots at the seedling stage, spraying at the tillering stage, injecting at the booting stage, and again spraying at the flowering stage to determine the appropriate artificial inoculation time. The panicle sheath instillation method and injection inoculation method were compared. The results show that stages 6 to 8 of young panicle differentiation are an important period for U. virens infection. There were no significant differences in the mean rates of infected panicles, mean rates of infected grains, and maximum infected grains per panicle between the two inoculation methods. However, the frequency of RFS ball occurrence at the upper part of the panicles was significantly higher on the spikelets inoculated by the injection method than that of spikelets inoculated by natural infection and panicle sheath instillation. Therefore, panicle sheath instillation method was more similar to the natural infection of U. virens in the field. This research exhibited an innovative artificial inoculation method for identification of U. virens pathogenicity and evaluation of rice resistance against RFS.

Application of Gene Editing Technology on Germplasm Resources

2018 ◽  
Vol 20 (6) ◽  
pp. 82
Author(s):  
Hongbing Han ◽  
Kabin Xie ◽  
Gang Cao ◽  
Jianhui Tian ◽  
Dong Wang
Keyword(s):  
Gene Editing ◽  
Germplasm Resources

Seasonal wound presence and susceptibility to Neonectria ditissima infection in New Zealand apple trees

2015 ◽  
Vol 68 ◽  
pp. 250-256 ◽  
Author(s):  
N.T. Amponsah ◽  
M. Walter R.M. Beresford ◽  
R.W.A. Scheper
Keyword(s):  
New Zealand ◽  
Artificial Inoculation ◽  
Early Winter ◽  
Leaf Fall ◽  
Apple Orchards ◽  
Apple Trees ◽  
Leaf Scar ◽  
Fruit Thinning ◽  
Different Types

Leaf scar wounds are important sites for Neonectria ditissima infection of apple trees Monitoring leaf fall in Scilate/Envy and Braeburn trees to estimate leaf scar wound presence showed maximum leaf scar incidence occurred in June (early winter) Wounds detected in New Zealand apple orchards were bud scale scars fruit thinning and picking wounds leaf scars and pruning cuts Picking wounds are caused during harvest where the pedicel is detached from the shoot Susceptibility of these different types of wounds was determined using artificial inoculation of N ditissima conidia during the season Pruning cut wounds were the most susceptible followed by fruit picking and thinning wounds and the least susceptible were leaf scar wounds No infections were observed when bud scale wounds were inoculated There was no difference in wound susceptibility between cultivars but overall Scilate/Envy wounds developed more lesions than Braeburn wounds

Genetic Diversity of Gulin-niupicha [Camellia sinensis(L.)O. Kuntze] Germplasm Resources

2014 ◽  
Vol 40 (12) ◽  
pp. 2118
Author(s):  
Shao-Jie LIU ◽  
Lin CHI ◽  
Wen-Gang XIE ◽  
Nan HAN ◽  
Ying-Hui CHEN ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Camellia Sinensis ◽  
Germplasm Resources
Sign in / Sign up

Export Citation Format

Share Document