Genetic Diversity of Gulin-niupicha [Camellia sinensis(L.)O. Kuntze] Germplasm Resources

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

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Genetic diversity and sampling strategy of Scutellaria baicalensis germplasm resources based on ISSR

Genetic Resources and Crop Evolution ◽

10.1007/s10722-012-9949-9 ◽

2013 ◽

Vol 60 (5) ◽

pp. 1673-1685 ◽

Cited By ~ 10

Author(s):

Chengke Bai ◽

Miaomiao Wen ◽

Longjin Zhang ◽

Guishuang Li

Keyword(s):

Genetic Diversity ◽

Sampling Strategy ◽

Scutellaria Baicalensis ◽

Germplasm Resources

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Measurement of Genetic Diversity of Chinese Seashore Paspalum Resources through Morphological and Sequence-related Amplified Polymorphism Analysis

Journal of the American Society for Horticultural Science ◽

10.21273/jashs04700-19 ◽

2019 ◽

Vol 144 (6) ◽

pp. 379-386

Author(s):

Yan Liu ◽

Hailin Guo ◽

Yi Wang ◽

Jingang Shi ◽

Dandan Li ◽

...

Keyword(s):

United States ◽

Genetic Diversity ◽

Cluster Analysis ◽

Genetic Variation ◽

Genetic Distance ◽

Morphological Characteristics ◽

The United States ◽

Germplasm Resources ◽

Seashore Paspalum ◽

Chinese Germplasm

Seashore paspalum (Paspalum vaginatum) is a notable warm-season turfgrass. Certain germplasm resources are distributed in the southern regions of China. The objectives of this study were to investigate the genetic diversity and genetic variation of Chinese seashore paspalum resources. Morphological characteristics and sequence-related amplified polymorphism (SRAP) markers were used to assess genetic relationships and genetic variation among 36 germplasm resources from China and six cultivars from the United States. The results showed significant variation for 13 morphological characteristics among 42 tested seashore paspalum accessions, and that the phenotypic cv was, in turn, turf height > turf density > internode length > inflorescence density > leaf width > reproductive branch height > spikelet width > leaf length > spikelet number > inflorescence length > internode diameter > inflorescence width > spikelet length. According to the morphological characteristics and cluster analysis, 42 seashore paspalum accessions were divided into six morphological types. In total, 374 clear bands were amplified using 30 SRAP primer combinations; among these bands, 321 were polymorphic with 85.83% polymorphism. SRAP marker cluster analysis showed that 42 seashore paspalum accessions were grouped into seven major groups, with a genetic similarity coefficient ranging from 0.4385 to 0.9893 and genetic distance values ranging from 0.0108 to 0.8244. The high level of genetic diversity occurred among Chinese germplasm, and the genetic distance was relatively high between Chinese germplasm and cultivars introduced from the United States. The patterns in morphological trait variations and genetic diversity will be useful for the further exploitation and use of Chinese seashore paspalum resources.

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Genetic diversity and population structure of Hemibagrus guttatus (Bagridae, Siluriformes) in the larger subtropical Pearl River based on COI and Cyt b genes analysis

Annales de Limnologie - International Journal of Limnology ◽

10.1051/limn/2021005 ◽

2021 ◽

Vol 57 ◽

pp. 7

Author(s):

Tianxu Kuang ◽

Fangmin Shuai ◽

Xinhui Li ◽

Weitao Chen ◽

Sovan Lek

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Sustainable Use ◽

Haplotype Diversity ◽

Pearl River ◽

Germplasm Resources ◽

Geographical Populations ◽

Commercially Important ◽

The Pearl River ◽

F Statistics

Understanding the genetic diversity and population structure of fish species is crucial for the sustainable use and protection of fish germplasm resources. Hemibagrus guttatus (Bagridae, Siluriformes) is widely distributed in the large subtropical Pearl River (China) and is commercially important. It's population have been declining. The genetic diversity of wild H. guttatus is not clear, despite its important ecological significance. In this paper, genes mitochondrial cytochrome c oxidase subunit I (COI) and cytochrome b (Cyt b) were used to analyze the genetic structure of H. guttatus population collected from six geographical populations in the main streams of the Pearl River. The results showed that the nucleotide diversity (π) and haplotype diversity (Hd) of wild H. guttatus was low (π < 0.005; Hd < 0.5). In addition, H. guttatus haplotypes did not cluster into clades according to geographical distribution, as revealed by neighbor-joining tree analysis. Analysis of molecular variance analysis (AMOVA) and F-statistics (Fst) values showed high homogeneity among wild H. guttatus populations. Our results suggest that there is degradation in germplasm resources of H. guttatus that could destabilize the sustainable use of this species and there was an urgent need for conservation of this species in South China.

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Genetic diversity of 324 cultivated tomato germplasm resources using agronomic traits and InDel markers

Euphytica ◽

10.1007/s10681-019-2391-8 ◽

2019 ◽

Vol 215 (4) ◽

Cited By ~ 4

Author(s):

Lan Jin ◽

Liping Zhao ◽

Yinlei Wang ◽

Rong Zhou ◽

Liuxia Song ◽

...

Keyword(s):

Genetic Diversity ◽

Agronomic Traits ◽

Germplasm Resources ◽

Indel Markers

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A Genetic Evaluation System for New Zealand White Rabbit Germplasm Resources Based on SSR Markers

Animals ◽

10.3390/ani10081258 ◽

2020 ◽

Vol 10 (8) ◽

pp. 1258

Author(s):

Jiali Li ◽

Bin Zhao ◽

Yang Chen ◽

Bohao Zhao ◽

Naisu Yang ◽

...

Keyword(s):

Genetic Diversity ◽

New Zealand ◽

Evaluation System ◽

Zealand White Rabbit ◽

Genetic Evaluation ◽

Germplasm Resources ◽

Polymorphic Loci ◽

Marker Combination ◽

New Zealand White Rabbits ◽

Two Generations

At present, there is an abundance of quality domestic rabbit breeds in China. However, due to the lack of technical standards for the genetic evaluation of rabbit germplasm resources, there have been a number of problems, such as poor breed conservation. By studying the genetic diversity of 130 New Zealand white rabbits (regardless of generation), we obtained the best simple sequence repeat (SSR) marker combination. We found that, when using microsatellite markers for the effective genetic evaluation of domestic rabbits, the number of records should be greater than 60 and the marker number more than 22. Through the comparative analysis of 30 combinations of 22 markers, the optimal combination of 22 markers was determined, and the 22 SSR polymorphic loci were distributed on different chromosomes. We performed a genetic analysis of 200 New Zealand white rabbits corresponding to two generations, using the best SSR polymorphic loci combination. There were no significant differences in the genetic diversity parameters between the two generations of rabbits (p > 0.05), indicating that the characteristics of this excellent rabbit germplasm have been effectively preserved. At the same time, we verified that the established method can be used to evaluate the breed conservation of rabbit germplasm resources.

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A new set of microsatellite loci for Cattleya walkeriana Gardner, an endangered tropical orchid species and its transferability to Cattleya loddigesii Lindl. and Cattleya nobilior Reichenbach

Plant Genetic Resources ◽

10.1017/s1479262117000193 ◽

2017 ◽

Vol 16 (3) ◽

pp. 284-287

Author(s):

Bruno C. Rossini ◽

Mariá B. Kampa ◽

Celso L. Marino ◽

Fernanda Bortolanza Pereira ◽

Fábio M. Alves ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Microsatellite Loci ◽

Null Alleles ◽

Germplasm Resources ◽

Significant Linkage Disequilibrium ◽

Orchid Species ◽

Expected Heterozygosity ◽

Tropical Orchid ◽

Significant Linkage

AbstractIn this study, we isolate and analyse a new set of microsatellite loci for Cattleya walkeriana. Twenty-two primer pairs were screened for C. walkeriana (n = 32) and assessed for their transferability to Cattleya loddigesii (n = 12) and Cattleya nobilior (n = 06). All loci amplified for C. walkeriana; however, for C. loddigesii and C. nobilior, four and five primers, respectively, did not present amplification. The polymorphic loci presented between 2 and 13 alleles per locus for both C. walkeriana and C. loddigesii, with respective averages of 5.1 and 4.2. For C. nobilior, we found between two and five alleles per locus, with an average of 2.6. For C. walkeriana, observed heterozygosity varied from 0.100 to 0.966, whereas expected heterozygosity ranged from 0.097 to 0.900. The observed and expected heterozygosity for C. loddigesii and C. nobilior were also estimated. We found no significant linkage disequilibrium between any pair of loci, and evidence of null alleles at four loci (Cw16, Cw24, Cw30 and Cw31) for C. walkeriana. The combined power to exclude the first parent and combined non-exclusion probability of identity were 0.999 and 2.3 × 10−20, respectively. These new loci can be used in studies of germplasm resources, and assessments of genotypic and genetic diversity and population structure, thus improving the accuracy of such analyses and their applicability in the conservation and protection of these endangered species.

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Genetic differences and variation in polysaccharide antioxidant activity found in germplasm resources for Job’s tears (Coix lacryma-jobi L.)

Botany ◽

10.1139/cjb-2019-0182 ◽

2020 ◽

Vol 98 (11) ◽

pp. 651-660

Author(s):

Lingyan Xuan ◽

Xiujie Xi ◽

Zixian Xu ◽

Huijun Xie ◽

Yunguo Zhu ◽

...

Keyword(s):

Genetic Diversity ◽

Antioxidant Activity ◽

Dna Sequences ◽

Antioxidant Activities ◽

Genetic Differences ◽

Germplasm Resources ◽

Factors Affecting ◽

Ssr Analysis ◽

Job’S Tears

Job’s tears (Coix lacryma-jobi L.) is an ancient plant with high nutritional and medicinal value. In this study, using 11 Chinese germplasm resources for Job’s tears, we examined genetic differences among the germplasms and differences in the in vitro antioxidant activities of coixan, and sought to identify inter-relationships between these two variables. We found that the intraspecific conservation of DNA sequences was high, with ITS regions and cpDNA trnL-F and trnH-psbA non-coding sequences showing no sequence variation, whereas the GBSSI gene showed a certain degree of variation among the different germplasms. EST-SSR analysis also revealed a relatively low level of genetic diversity among the germplasms. Coixan was shown to be an efficient antioxidant, and among the germplasms examined, the LNYX, FJPC, and AHBZ had the highest antioxidant activities. However, none of the four in vitro antioxidant activity indices we assessed were significantly correlated with the geographical origin of the germplasm (latitude and longitude); however, one of them was significantly associated with genetic diversity. Although the factors affecting the antioxidant activity of coixan are complex, the role of heredity should not be ignored. Our findings have implications for the scientific evaluation, identification, and sustainable utilization of the germplasm resources for Job’s tears.

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