Genetic diversity of physic nut (Jatropha curcas L.) revealed by SSR markers

2011 ◽  
Vol 14 (2) ◽  
pp. 105-110 ◽  
Author(s):  
Yaowalak Na-ek ◽  
Arunee Wongkaew ◽  
Thitaporn Phumichai ◽  
Nongluck Kongsiri ◽  
Rungsarid Kaveeta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Jatropha Curcas ◽  
Physic Nut ◽  
Jatropha Curcas L

scholarly journals Analysis of the genetic diversity of physic nut, Jatropha curcas L. accessions using RAPD markers

2012 ◽  
Vol 39 (6) ◽  
pp. 6505-6511 ◽  
Author(s):  
M. Y. Rafii ◽  
M. Shabanimofrad ◽  
M. W. Puteri Edaroyati ◽  
M. A. Latif
Keyword(s):  
Genetic Diversity ◽  
Jatropha Curcas ◽  
Rapd Markers ◽  
Physic Nut ◽  
Jatropha Curcas L

scholarly journals Developmenrt of EST-SSR and genomic-SSR markers to assess genetic diversity in Jatropha Curcas L.

2010 ◽  
Vol 3 (1) ◽  
Author(s):  
Mingfu Wen ◽  
Haiyan Wang ◽  
Zhiqiang Xia ◽  
Meiling Zou ◽  
Cheng Lu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Jatropha Curcas ◽  
Jatropha Curcas L ◽  
Genomic Ssr

scholarly journals PENGEMBANGAN MARKA SIMPLE SEQUENCE REPEAT UNTUK Jatropha spp.

2020 ◽  
Vol 17 (4) ◽  
pp. 140
Author(s):  
DARMAWAN SAPTADI ◽  
R.R. SRI HARTATI ◽  
ASEP SETIAWAN ◽  
BAMBANG HELIYANTO ◽  
SUDARSONO SUDARSONO
Keyword(s):  
Ssr Markers ◽  
Jatropha Curcas ◽  
Simple Sequence Repeat ◽  
Genomic Dna ◽  
Ssr Marker ◽  
Sequence Repeat ◽  
Physic Nut ◽  
Jatropha Curcas L ◽  
Dna Database ◽  
Simple Sequence

<p>ABSTRAK</p><p>Pemuliaan tanaman jarak pagar (Jatropha curcas L.) untukmenghasilkan varietas berdaya hasil dan berkadar minyak tinggi perludilakukan. Penggunaan marka molekuler dapat membantu mempercepattercapainya tujuan pemuliaan tanaman jarak pagar. Marka simple sequencerepeat (SSR) merupakan marka ko-dominan yang efektif untuk mendu-kung program pemuliaan tanaman, tetapi penerapannya pada jarak pagarmasih terbatas. Penelitian yang dilakukan bertujuan untuk : (i) merancangprimer spesifik SSR menggunakan aksesi DNA jarak pagar yang tersediadi GenBank DNA database dan (ii) mengevaluasi efektivitas pasanganprimer yang dirancang untuk menghasilkan marka SSR yang polimorfikuntuk jarak pagar dan J. multifida. Dua puluh delapan pasang primerspesifik SSR telah berhasil dirancang menggunakan aksesi DNA asal jarakpagar yang ada di GenBank DNA database. DNA genomik jarak pagar danJ. multifida yang diisolasi dapat digunakan sebagai templat untukamplifikasi PCR. Dari 28 pasang primer yang dikembangkan, semuanyamampu menghasilkan marka SSR dari genom jarak pagar dan hanya 19pasang primer yang menghasilkan marka SSR dari genom J. multifida.Dari 19 pasangan primer spesifik SSR yang dievaluasi mampu dihasilkan44 alel dengan ukuran produk amplifikasi berkisar antara 100-360 bp.Sebanyak 35 alel (79,5%) yang diamati merupakan alel yang polimorfik.Marka SSR yang didapatkan tidak polimorfik intra-aksesi jarak pagar atauintra-aksesi J. multifida tetapi polimorfik untuk inter-aksesi kedua spesies.Karena marka SSR yang dihasilkan bersifat polimorfik untuk aksesi jarakpagar dengan aksesi J. multifida maka dapat digunakan sebagai markauntuk mendeteksi hasil persilangan F 1 inter-spesies J. curcas x J. multifida.</p><p>Kata kunci : Jatropha curcas L., jarak pagar, J. multifida, DNA berulang,rancangan primer</p><p>ABSTRACT</p><p>Development of Simple Sequence Repeat Markers forJatropha spp.</p><p>Breeding of physic nut (Jatropha curcas L.) to obtain new varietiesthat are high in yield and oil content needs to be conducted. Molecularmarker could be used to assist breeding of physic nut (J. curcas). Simplesequence repeat (SSR) marker is a co-dominant marker and theoretically itcould be used to support physic nut breeding program. However, onlylimited information has been available regarding molecular analysis ofphysic nut. The objectives of this research were: (i) to design SSR specificprimer based on DNA sequences available in the GenBank DNA databaseand (ii) to evaluate effectiveness of the primer pairs to produce polymor-phic SSR markers for J. curcas and J. multifida. Twenty eight primer pairswere designed and developed using physic nut DNA available in theGenBank DNA database. Total genomic DNA isolated from J. curcas andJ. multifida could be used as DNA templates for PCR amplification. Of the28 primer pairs developed in this research yielded SSR marker using J.curcas genomic DNA, while only 19 out of 28 pairs yielded SSR markersusing J. multifida genomic DNA. As many as 44 alleles with the size ofamplified products ranged from 100-360 bp were identified. Thirty fivealleles (79.5%) out of 44 identified ones were polymorphic. Results ofanalysis indicated that identified SSR markers generated using thedesigned primers were not polymorphic intra accession of J. curcas norintra-accession of J. multifida either. However, the generated SSR markerswere polymorphic for inter-accession of the two Jatropha species. Sincethe generated markers were only polymorphic for J. curcas and J.multifida, they could be used as markers for identifying interspecific F 1hybrids derived from crossing between J. curcas and J. multifida.</p><p>Key words: Jatropha curcas L., physic nut, J. multifida, DNA repeatsequence, primer design</p>

scholarly journals Morphophysiological Traits as Indicators of Genetic Diversity in Brazilian Populations of Jatropha curcas L. Grown Under Water Deficit

2020 ◽  
Vol 8 (3) ◽  
pp. 527
Author(s):  
Leandro Dias da Silva ◽  
Fábio Pinto Gomes ◽  
Mateus Pires Barbosa ◽  
Raul Antônio Araújo do Bonfim ◽  
Mikaela Oliveira Souza ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plant Height ◽  
Principal Components ◽  
Jatropha Curcas ◽  
Biodiesel Production ◽  
Physiological Traits ◽  
Guaiacol Peroxidase ◽  
Jatropha Curcas L ◽  
Root Area ◽  
Genetic Dissimilarity

Jatropha curcas L. (Euphorbiaceae) is a species grown in tropicalcountries and used for biodiesel production. Morphophysiological traits were analyzed to assess the genetic diversity in nine genotypes of J. curcas under deficit water. Seeds of plants from different populations, collected in diferente brazilian regions, were grown under two water regimes (100% and 50% tank capacity). Multivariate analysis was used to characterize genetic diversity. The UPGMA dendrogram built from the genetic distance group indicated the segregation of genotypes into five groups for growth traits and six groups for physiological traits. Then, an principal components analysis was carried out, to evaluate the pattern of character variations and then segregate the characteristics that could distinguish parental genotypes for use in plant breeding. Results showed 65.50% and 56.02% for the two first principal components of growth and physiological traits, respectively. Total root area was the most determining trait for genetic dissimilarity (18.9%) and group formation, followed by plant height (17.9%) and number of leaves (17.6%). On the other hand, stomatal conductance (gs) (24.9%) and guaiacol peroxidase (GPX) (20.9%) were the most determining physiological traits. The analysis of morphophysiological traits indicated CNPAE-298 and 299 as the most distant genotypes among the plant groups. Under water-limited conditions, total root area, plant height, gs and GPX were the most efficient traits to explain genetic dissimilarity among the genotypes, and for this reason they should be referred as a priority for further studies on genotypes selection in this species.

scholarly journals Estimation of outcrossing rates in interspecific backcross plants of Jatropha curcas (L.) using AFLP and SSR markers

2015 ◽  
Vol 21 (4) ◽  
pp. 605-609 ◽  
Author(s):  
Pratima Sinha ◽  
Md Aminul Islam ◽  
Madan Singh Negi ◽  
Shashi Bhushan Tripathi
Keyword(s):  
Ssr Markers ◽  
Jatropha Curcas ◽  
Interspecific Backcross ◽  
Jatropha Curcas L ◽  
Outcrossing Rates

The genetic diversity of Jatropha Curcas (L.) germplasm in Ghana as revealed by random amplified polymorphic DNA (RAPD) primers

2012 ◽  
Vol 86 (3) ◽  
pp. 443-450 ◽  
Author(s):  
E. Owusu Danquah ◽  
R. Akromah ◽  
S. J. Quashie-Sam ◽  
W. Oduro ◽  
D. Falk ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Jatropha Curcas ◽  
Random Amplified Polymorphic Dna ◽  
Jatropha Curcas L

scholarly journals Leaf sampling to assess mineral nutrient composition of physic nut plants (Jatropha curcas L.)

2016 ◽  
Vol 10 (8) ◽  
pp. 1069-1074
Author(s):  
Rosiane de Lourdes Silva de Lima ◽  
◽  
Carlos Alberto Vieira de Azevedo ◽  
Hans Raj Gheyi ◽  
Jairo Osvaldo Cazetta ◽  
...  
Keyword(s):  
Jatropha Curcas ◽  
Nutrient Composition ◽  
Mineral Nutrient ◽  
Physic Nut ◽  
Jatropha Curcas L ◽  
Leaf Sampling
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