Notes on rapid diagnostic tests for chronic Chagas disease

2017 ◽  
Vol 110 (1) ◽  
pp. 9-12 ◽  
Author(s):  
A. Angheben ◽  
F. Gobbi ◽  
D. Buonfrate ◽  
S. Tais ◽  
M. Degani ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Diagnostic Tests ◽  
Rapid Diagnostic Tests ◽  
Chronic Chagas Disease

scholarly journals International meeting: new diagnostic tests are urgently needed to treat patients with Chagas disease

2008 ◽  
Vol 41 (3) ◽  
pp. 315-319 ◽  
Keyword(s):  
Chagas Disease ◽  
Diagnostic Tests ◽  
Clinical Manifestations ◽  
Specific Treatment ◽  
Treatment Access ◽  
Limited Effectiveness ◽  
Technical Report ◽  
Blood Banks ◽  
Chronic Chagas Disease ◽  
Cruzi Infection

Trypanosoma cruzi infection is often not detected early on or actively diagnosed, partly because most infected individuals are either asymptomatic or oligosymptomatic. Moreover, in most places, neither blood banks nor healthcare units offer diagnostic confirmation or treatment access. By the time patients present clinical manifestations of advanced chronic Chagas disease, specific treatment with current drugs usually has limited effectiveness. Better-quality serological assays are urgently needed, especially rapid diagnostic tests for diagnosis patients in both acute and chronic phases, as well as for confirming that a parasitological cure has been achieved. Some new antigen combinations look promising and it is important to assess which ones are potentially the best, together with their requirements in terms of investigation and development. In August 2007, a group of specialized researchers and healthcare professionals met to discuss the state of Chagas infection diagnosis and to build a consensus for a plan of action to develop efficient, affordable, accessible and easy-to-use diagnostic tests for Chagas disease. This technical report presents the conclusions from that meeting.

scholarly journals Comparison of seven diagnostic tests to detect Trypanosoma cruzi infection in patients in chronic phase of Chagas disease

2014 ◽  
pp. 61-66 ◽  
Author(s):  
Luisa Fernanda Duarte ◽  
Oscar Roberto Flórez ◽  
Giovanna Rincón ◽  
Clara Isabel González
Keyword(s):  
Comparative Analysis ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Diagnostic Tests ◽  
Nuclear Dna ◽  
Quality Analysis ◽  
Control Group ◽  
Predictive Values ◽  
Chronic Chagas Disease ◽  
Cruzi Infection

Objective: To compare the diagnostic performance of seven methods to determine Trypanosoma cruzi infection in patients with chronic Chagas disease. Methods: Analytical study, using the case-control design, which included 205 people (patients with Chagasic cardiomyopathy, n= 100; control group, n= 105). Three enzyme linked immunosorbent assays, one indirect hemagglutination assay and one immunochromatographic test were assessed. Additionally, DNA amplification was performed via the PCR method using kinetoplast and nuclear DNA as target sequences. For the comparative analysis of diagnostic tests, the parameters used were sensitivity, specificity, positive and negative predictive values, Receiver Operator Characteristic (ROC), positive and negative likelihood ratio, as well as κ quality analysis. Results: The commercial Bioelisa Chagas test showed the highest sensitivity (98%), specificity (100%), and positive and negative predictive values; additionally it had the highest discriminatory power. Otherwise, the amplification of T. cruzi DNA in blood samples showed low values of sensitivity (kinetoplast DNA= 51%, nuclear DNA= 22%), but high values of specificity (100%), and moderate to low discriminatory ability. Conclusion: The comparative analysis among the different methods suggests that the diagnostic strategy of T. cruzi infection in patients with chronic Chagas disease can be performed using ELISA assays based on recombinant proteins and/or synthetic peptides, which show higher diagnosis performance and can confirm and exclude the diagnosis of T. cruzi infection. The molecular methods show poor performance when used in the diagnosis of patients with chronic Chagas disease.

scholarly journals Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis

2017 ◽  
Vol 11 (4) ◽  
pp. e0005501 ◽  
Author(s):  
Karina E. Egüez ◽  
Julio Alonso-Padilla ◽  
Carolina Terán ◽  
Zenobia Chipana ◽  
Wilson García ◽  
...  
Keyword(s):  
Chagas Disease ◽  
Diagnostic Tests ◽  
Disease Diagnosis ◽  
Rapid Diagnostic Tests

scholarly journals Simultaneous use of two rapid diagnostic tests for the diagnosis of Chagas disease

2018 ◽  
Vol 49 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Diego Mendicino ◽  
Carlina Colussi ◽  
Edgardo Moretti
Keyword(s):  
Chagas Disease ◽  
Diagnostic Tests ◽  
Rapid Diagnostic Tests ◽  
Validity And Reliability ◽  
Serum Samples ◽  
Combined Use ◽  
Single Use ◽  
Simultaneous Use ◽  
Κ Statistic

The sensitivity of rapid diagnostic tests (RDT) for Chagas disease is not great enough for their single use. The aim of this paper was to evaluate the performance of two RDTs for Chagas disease, used simultaneously. Two different RDTs (A and B) were performed in 64 and 42 serum samples that were negative and positive, respectively, by conventional serological techniques. Validity and reliability of both tests were evaluated individually and simultaneously. Sensitivity was 90.5% and 97.6%, and specificity was 100% and 93.8%, for RDT A and B, respectively. The κ statistic was 0.96. When both RDTs were used simultaneously, sensitivity was 97.4%, specificity was 100% and the discordance percentage 6.6%. The combined use of two RDTs with serum samples is an acceptable application in healthcare centres.

Chronic Wasting Disease Options of Surveillance in Carpathian Cervids: from the Identification of Characteristic Microscopic Pathologic Changes to Prionic Seeded Conversion and Amplification Assays

2001 ◽  
Vol 71 (3) ◽  
pp. 480-486
Author(s):  
Florica Barbuceanu ◽  
Stelian Baraitareanu ◽  
Stefania-Felicia Barbuceanu ◽  
Gabriel Predoi
Keyword(s):  
Enzyme Immunoassay ◽  
Diagnostic Tests ◽  
Chronic Wasting Disease ◽  
Rapid Diagnostic Tests ◽  
Diagnostic Methods ◽  
Wasting Disease ◽  
Western Immunoblotting ◽  
Negative Results ◽  
Antigen Capture ◽  
Confirmatory Tests

This paper describes the current diagnostic methods of Chronic Wasting Disease (CWD) in cervides used between 2013 and 2017 in Romania. The active surveillance of CWD involves the targeted groups screening by using rapid diagnostic tests (e.g., antigen capture enzyme immunoassay). If the first test does not provide certain negative results, then the confirmatory methods have been used, i.e. histopathology, immunohistochemistry and Western immunoblotting. These tests did not lead to the detection of CWD prions (PrPCWD) in Romania. This may be due to the absence or insufficient quantity of PrPCWD in samples, below the threshold of confirmatory tests.

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