Keratinase production by Bacillus pumilus GHD in solid-state fermentation using sugar cane bagasse: optimisation of culture conditions using a Box-Behnken experimental design

2011 ◽  
Vol 61 (3) ◽  
pp. 663-672 ◽  
Author(s):  
Ghada E. A. Awad ◽  
Mona A. Esawy ◽  
Walla A. Salam ◽  
Bassem M. Salama ◽  
Amal F. Abdelkader ◽  
...  
1998 ◽  
Vol 5 (1-4) ◽  
pp. 75-78 ◽  
Author(s):  
J Cordova ◽  
M Nemmaoui ◽  
M Ismaı̈li-Alaoui ◽  
A Morin ◽  
S Roussos ◽  
...  

Author(s):  
Michele Rigon Spier ◽  
Adenise Lorenci Woiciechowski ◽  
Luciana Porto de Souza Vandenberghe ◽  
Carlos Ricardo Soccol

The use of agro industrials products cassava starch and sugar cane bagasse present a great potential as substrate and support, respectively, showing low production costs for amylases production under solid-state fermentation by Aspergillus niger LPB 28. The effect of various factors was examined and some variables were optimized to ?-amylase and amyloglucosidase production. Inoculum rate in different concentrations (105, 106, 107 and 108 spores/g of cassava starch) were used to inoculate the solid substrate for the SSF. The material was supplemented with nitrogen sources; calcium source and micronutrients solution. The ratios of cassava starch to sugar cane bagasse (1/1; 1.5/1; 1/1.5; 1/2; 2/1; 1/3; 3/1) were tested and the initial moisture content varying 80, 85 and 90%. Cultivation was carried out at temperatures 25, 30 and 35ºC for 48, 60 and 72 h. Study of influence of pH initial in SSF was conduced in pH 4.0, 5.0, 6.0, 7.0 and 8.0. Statistical analysis was carried out using the software STATISTICA 5.1 (StatSoft, Tulsa, OK, USA). A 32 complete factorial design (2 factors, 3 levels and 9 experiments) was used to study the effect of the factors in ?-amylase and amyloglucosidase production. The results showed cassava starch and sugar cane bagasse at the ratios of 2/1 was the best for optimum production of ?-amylase and amyloglucosidase. The maximum yield (?-amylase 1732.95 U/g of cassava starch and amyloglucosidase 2044.94 U/g of cassava starch) was achieved with optimized process parameters such as incubation period (60 hours), moisture level was 90%, inoculation rate (105 spores/g of dried material), pH 4.0 and fermentation temperature (30ºC). The nitrogen source (KNO3) also enhanced the ?-amylase and amyloglucosidase activities. In kinetic characterization of enzymes the Michaelis-Menten constant KM and maximum velocity Vmax (KM 10.84 g/l, Vmax 3.42 g/l) for ?-amylase and KM 11.32 g/l and Vmax 5.18 g/l for amyloglucosidase.


1994 ◽  
Vol 16 (8) ◽  
pp. 865-870 ◽  
Author(s):  
Olga Bravo ◽  
Alexis Ferrer ◽  
Cateryna Aiello ◽  
Anselmo Ledesma ◽  
Miguel D�vila

1975 ◽  
Vol 17 (2) ◽  
pp. 291-293 ◽  
Author(s):  
K. Lakshminarayana ◽  
K. Chaudhary ◽  
S. Ethiraj ◽  
P. Tauro

2014 ◽  
Vol 45 (2) ◽  
pp. 373-377 ◽  
Author(s):  
F. Veana ◽  
J.L. Martínez-Hernández ◽  
C.N. Aguilar ◽  
R. Rodríguez-Herrera ◽  
G. Michelena

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