Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production

2011 ◽  
Vol 62 (2) ◽  
pp. 753-763 ◽  
Author(s):  
Rashmi Rekha Saikia ◽  
Suresh Deka ◽  
Manab Deka ◽  
Ibrahim M. Banat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soil ◽  
Nitrogen Sources ◽  
Biosurfactant Production

scholarly journals Biosurfactant production by Pseudomonas aeruginosa isolated from aquaculture farm soil and its optimisation

2018 ◽  
Vol 65 (4) ◽  
Author(s):  
Ranjit kumar Nadella ◽  
Murugadas Vaiyapuri ◽  
Ahamed Basha kusunur ◽  
Toms Cheriath Joseph ◽  
Lalitha Kuttanappilly Velayudhan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Yeast Extract ◽  
Bacterial Isolate ◽  
Bacterial Biomass ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Screening Methods ◽  
Emulsification Index ◽  
Aquaculture Farm ◽  
Biochemical Characterisation

In the present study, aquaculture farm soil was screened for the biosurfactant producing bacteria. Total of 43 distinct morphological colonies were isolated from the farm soil and their biosurfactant production was evaluated by employing different screening methods. Fourteen biosurfactant producing bacterial isolates were selected based on the formation of dark blue halos on CTAB agar, emulsification index, oil spreading assay and BATH assay. Based on the results, bacterial isolate (BHA 9) showed highest production of biosurfactant and selected for further studies. Biochemical characterisation revealed that the bacterial isolate responsible for biosurfactant production is Gram negative, slender long rod shape bacteria and oxidase and catalase positive. Molecular characterisation of 16S r-DNArevealed that it belongs to Pseudomonas aeruginosa . Optimization studies were carried out at different temperatures (25, 30, 35 and 40 o C) using four different carbon sources (1%) i.e ., glucose, sucrose, maltose and starch and four nitrogen sources (1%) viz ., peptone, ammonium nitrate, beef extract and yeast extract at different pH (6, 7, 8, 9 and 10) and NaCl levels (0.50, 1, 1.50 and 2%). Emulsification index and the bacterial biomass (OD 600 ) were recorded at 24, 48, 72 and 96 h intervals. Optimum condition for biosurfactant production by this bacterium was achieved when glucose and yeast extract was used as carbón and nitrogen sources, respectively  maintaining a temperature of 35 o C, pH 8 and NaCl 1.5% measured in terms of emulsification index and bacterial biomass. This is the first reported study for the biosurfactant producing bacteria from aquaculture farm soil which may find its application in various fields.

scholarly journals Factorial Design to Optimize Biosurfactant Production byYarrowia lipolytica

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Gizele Cardoso Fontes ◽  
Priscilla Filomena Fonseca Amaral ◽  
Marcio Nele ◽  
Maria Alice Zarur Coelho
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Standard Process ◽  
Emulsification Index ◽  
Full Factorial

In order to improve biosurfactant production byYarrowia lipolyticaIMUFRJ 50682, a factorial design was carried out. A24full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (ΔST) and emulsification index (EI). The best results (67.7% of EI and 20.9 mNm−1ofΔST) were obtained in a medium composed of 10 g 1−1of ammonium sulfate and 0.5 g 1−1of yeast extract. Then, the effects of carbon sources (glycerol, hexadecane, olive oil, and glucose) were evaluated. The most favorable medium for biosurfactant production was composed of both glucose (4% w/v) and glycerol (2% w/v), which provided an EI of 81.3% and aΔST of 19.5 mN m−1. The experimental design optimization enhancedΔEI by 110.7% andΔST by 108.1% in relation to the standard process.

scholarly journals Rhamnolipid Biosurfactant Production by Strains of Pseudomonas aeruginosa Using Low-Cost Raw Materials

2002 ◽  
Vol 18 (6) ◽  
pp. 1277-1281 ◽  
Author(s):  
K.S.M. Rahman ◽  
T.J. Rahman ◽  
S. McClean ◽  
R. Marchant ◽  
I.M. Banat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Raw Materials ◽  
Low Cost ◽  
Biosurfactant Production ◽  
Rhamnolipid Biosurfactant

Bioremediation of a Benzo[a]Pyrene-Contaminated Soil Using a Microbial Consortium with Pseudomonas aeruginosa, Candida albicans, Aspergillus flavus, and Fusarium sp.

2015 ◽  
Vol 226 (9) ◽  
Author(s):  
Dafne Q. Waszak ◽  
Ana Cristina B. da Cunha ◽  
Marcio R. A. Agarrallua ◽  
Cristine S. Goebel ◽  
Carlos H. Sampaio
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aspergillus Flavus ◽  
Contaminated Soil ◽  
Microbial Consortium ◽  
Fusarium Sp
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