Screening of the best carbon and nitrogen sources for pseudomonas aeruginosa as a potential polyhydroxyalkanoates (PHAs) and rhamnolipids producer

ABSTRACT The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.

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The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa

Molecular Microbiology ◽

10.1046/j.1365-2958.2001.02435.x ◽

2001 ◽

Vol 40 (4) ◽

pp. 917-931 ◽

Cited By ~ 137

Author(s):

Takayuki Nishijyo ◽

Dieter Haas ◽

Yoshifumi Itoh

Keyword(s):

Pseudomonas Aeruginosa ◽

Regulatory System ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Two Component

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Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production

Process Biochemistry ◽

10.1016/j.procbio.2010.05.032 ◽

2010 ◽

Vol 45 (9) ◽

pp. 1504-1510 ◽

Cited By ~ 19

Author(s):

Rodrigo Siqueira Reis ◽

Surza Lucia Gonçalves da Rocha ◽

Donat Alexander Chapeaurouge ◽

Gilberto Barbosa Domont ◽

Lidia Maria Melo Santa Anna ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Rhamnolipid Production

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Metabolism of Chicken Feathers and Concomitant Electricity Generation by Pseudomonas aeruginosa by Employing Microbial Fuel Cell (MFC)

Journal of Waste Management ◽

10.1155/2014/928618 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Venkatesh Chaturvedi ◽

Pradeep Verma

Keyword(s):

Pseudomonas Aeruginosa ◽

Fuel Cell ◽

Microbial Fuel Cell ◽

Nitrogen Sources ◽

Electricity Production ◽

Malt Extract ◽

Feather Degradation ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Chicken Feathers

Keratinolytic potential of Pseudomonas aeruginosa strain SDS3 has been evaluated for the metabolism of chicken feathers. Results indicated that strain SDS3 showed complete metabolism of 0.1 and 0.5% (w/v) chicken feathers in minimal medium. Feathers were metabolized up to 80% at 1% (w/v) concentration. Maximum soluble protein (480.8±17.1 μg/mL) and keratinase (15.4±0.25 U/mL) were observed in the presence of 1% chicken feathers after five days of incubation. The effect of carbon and nitrogen sources showed that feather degradation was stimulated by complex carbon/nitrogen sources such as starch, malt extract, tryptone, and beef extract and was inhibited by simple carbon and nitrogen sources. Electricity production by employing chicken feathers as a substrate in microbial fuel cell (MFC) was evaluated. It was observed that maximum voltage corresponding to 141 mV was observed after 14 days of incubation. Maximum power density of 1206.78 mW/m2 and maximum current density of 8.6 mA/m2 were observed. The results clearly indicate that chicken feathers can be successfully employed as a cheap substrate for electricity production in MFC. This is the first report showing employment of chicken feathers as substrate in MFC.

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