scholarly journals Factorial Design to Optimize Biosurfactant Production byYarrowia lipolytica

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Gizele Cardoso Fontes ◽  
Priscilla Filomena Fonseca Amaral ◽  
Marcio Nele ◽  
Maria Alice Zarur Coelho
Keyword(s):  
Surface Tension ◽  
Factorial Design ◽  
Ammonium Sulfate ◽  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Standard Process ◽  
Emulsification Index ◽  
Full Factorial

In order to improve biosurfactant production byYarrowia lipolyticaIMUFRJ 50682, a factorial design was carried out. A24full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (ΔST) and emulsification index (EI). The best results (67.7% of EI and 20.9 mNm−1ofΔST) were obtained in a medium composed of 10 g 1−1of ammonium sulfate and 0.5 g 1−1of yeast extract. Then, the effects of carbon sources (glycerol, hexadecane, olive oil, and glucose) were evaluated. The most favorable medium for biosurfactant production was composed of both glucose (4% w/v) and glycerol (2% w/v), which provided an EI of 81.3% and aΔST of 19.5 mN m−1. The experimental design optimization enhancedΔEI by 110.7% andΔST by 108.1% in relation to the standard process.

scholarly journals Effects of Nitrogen and Carbon Sources on Biosurfactant Production by Hydrocarbon-utilizing Stenotrophomonas sp.

2019 ◽  
pp. 1-10
Author(s):  
Victor Ezebuiro ◽  
Ipeghan Jonathan Otaraku ◽  
Boma Oruwari ◽  
Gideon Chijioke Okpokwasili
Keyword(s):  
Surface Tension ◽  
Olive Oil ◽  
Yeast Extract ◽  
Emulsion Stability ◽  
Carbon Sources ◽  
Glass Slide ◽  
Biosurfactant Production ◽  
Tension Reduction ◽  
Emulsification Index ◽  
Surface Tension Reduction

Aim: This study investigated effects of nitrogen and carbon sources on the production of biosurfactant by a hydrocarbon-utilizing bacterium, Stenotrophomonas sp. Methodology: The hydrocarbon-utilizing bacterium was isolated with Bushnell Haas (BH) broth using enrichment method. Biosurfactant production was screened by evaluating the following characteristics: Emulsification index (E-24), oil spreading (displacement), tilted glass slide, haemolysis on blood agar, and lipase production. Effects of combination of nitrogen sources (yeast extract and NH4NO3, yeast extract and urea, yeast extract and asparagine, yeast extract and peptone, NaNO3 and peptone, NaNO3 and asparagine, and yeast extract and NaNO3) and carbon sources (glucose, fructose, galactose, cassava peel, soya bran, olive oil, sucrose, crude oil, diesel and glycerol) on biosurfactant production were determined with emulsion stability and surface tension as responses. The bacterium was identified based on phenotypic, microscopic, and biochemical characteristics. Results: The isolate produced colonies on BH agar containing either naphthalene or hexadecane as sole source of carbon after 48-h incubation. Screening characteristics for the production of biosurfactant by the isolate were as follows: 46% emulsification index, 3.1 cm2 oil displacement, 1.8 cm zone of clearance on tributyrin agar, γ-haemolysis, and positive tilted glass slide. The best carbon source with the highest emulsion stability (51.6%) was fructose whereas the best surface tension reduction (30.85 mN/m) was observed with olive oil as carbon sources after 7 days of incubation. For nitrogen, the combination of yeast extract and NH4NO3 gave the highest emulsion stability (60.7%) and the best surface tension reduction (39.58 mN/m). The data obtained were significant at P<0.05 and the bacterial isolate identified as Stenotrophomonas sp. Conclusion: This study has demonstrated the ability of the hydrocarbon-utilizing bacterium, Stenotrophomonas sp. to produce biosurfactant, indicated by reduction of surface tension and formation of stable emulsion. This method of biosurfactant production can be further scaled up for industrial purpose. 

scholarly journals Biosurfactant production by Pseudomonas aeruginosa isolated from aquaculture farm soil and its optimisation

2018 ◽  
Vol 65 (4) ◽  
Author(s):  
Ranjit kumar Nadella ◽  
Murugadas Vaiyapuri ◽  
Ahamed Basha kusunur ◽  
Toms Cheriath Joseph ◽  
Lalitha Kuttanappilly Velayudhan ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Yeast Extract ◽  
Bacterial Isolate ◽  
Bacterial Biomass ◽  
Nitrogen Sources ◽  
Biosurfactant Production ◽  
Screening Methods ◽  
Emulsification Index ◽  
Aquaculture Farm ◽  
Biochemical Characterisation

In the present study, aquaculture farm soil was screened for the biosurfactant producing bacteria. Total of 43 distinct morphological colonies were isolated from the farm soil and their biosurfactant production was evaluated by employing different screening methods. Fourteen biosurfactant producing bacterial isolates were selected based on the formation of dark blue halos on CTAB agar, emulsification index, oil spreading assay and BATH assay. Based on the results, bacterial isolate (BHA 9) showed highest production of biosurfactant and selected for further studies. Biochemical characterisation revealed that the bacterial isolate responsible for biosurfactant production is Gram negative, slender long rod shape bacteria and oxidase and catalase positive. Molecular characterisation of 16S r-DNArevealed that it belongs to Pseudomonas aeruginosa . Optimization studies were carried out at different temperatures (25, 30, 35 and 40 o C) using four different carbon sources (1%) i.e ., glucose, sucrose, maltose and starch and four nitrogen sources (1%) viz ., peptone, ammonium nitrate, beef extract and yeast extract at different pH (6, 7, 8, 9 and 10) and NaCl levels (0.50, 1, 1.50 and 2%). Emulsification index and the bacterial biomass (OD 600 ) were recorded at 24, 48, 72 and 96 h intervals. Optimum condition for biosurfactant production by this bacterium was achieved when glucose and yeast extract was used as carbón and nitrogen sources, respectively  maintaining a temperature of 35 o C, pH 8 and NaCl 1.5% measured in terms of emulsification index and bacterial biomass. This is the first reported study for the biosurfactant producing bacteria from aquaculture farm soil which may find its application in various fields.

scholarly journals β-Fructofuranosidase andβ–D-Fructosyltransferase from NewAspergillus carbonariusPC-4 Strain Isolated from Canned Peach Syrup: Effect of Carbon and Nitrogen Sources on Enzyme Production

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Gustavo Carvalho do Nascimento ◽  
Ryhára Dias Batista ◽  
Claudia Cristina Auler do Amaral Santos ◽  
Ezequiel Marcelino da Silva ◽  
Fabrício Coutinho de Paula ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Soybean Protein ◽  
Low Cost ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Carbon And Nitrogen ◽  
Production Values ◽  
Pure Carbon ◽  
Fermentation Parameters

β-fructofuranosidase (invertase) andβ-D-fructosyltransferase (FTase) are enzymes used in industrial processes to hydrolyze sucrose aiming to produce inverted sugar syrup or fructooligosaccharides. In this work, a blackAspergillussp. PC-4 was selected among six filamentous fungi isolated from canned peach syrup which were initially screened for invertase production. Cultivations with pure carbon sources showed that invertase and FTase were produced from glucose and sucrose, but high levels were also obtained from raffinose and inulin. Pineapple crown was the best complex carbon source for invertase (6.71 U/mL after 3 days of cultivation) and FTase production (14.60 U/mL after 5 days of cultivation). Yeast extract and ammonium chloride nitrogen sources provided higher production of invertase (6.80 U/mL and 6.30 U/mL, respectively), whereas ammonium nitrate and soybean protein were the best nitrogen sources for FTase production (24.00 U/mL and 24.90 U/mL, respectively). Fermentation parameters for invertase using yeast extract wereYP/S= 536.85 U/g andPP= 1.49 U/g/h. FTase production showed values ofYP/S= 2,627.93 U/g andPP= 4.4 U/h using soybean protein. The screening for best culture conditions showed an increase of invertase production values by 5.10-fold after 96 h cultivation compared to initial experiments (fungi bioprospection), while FTase production increased by 14.60-fold (44.40 U/mL) after 168 h cultivation.A. carbonariusPC-4 is a new promising strain for invertase and FTase production from low cost carbon sources, whose synthesized enzymes are suitable for the production of inverted sugar, fructose syrups, and fructooligosaccharides.

scholarly journals Effect of media composition (carbon and nitrogen sources) on the production of bioemulsifier from Serratia marcescens S10

2012 ◽  
Vol 6 (2) ◽  
pp. 9-14
Author(s):  
Entissar Faroun Ahmed ◽  
Shatha Salman Hassan
Keyword(s):  
Surface Tension ◽  
Serratia Marcescens ◽  
Corn Oil ◽  
Nitrogen Sources ◽  
Sesame Oil ◽  
Source Surface ◽  
Carbon And Nitrogen Sources ◽  
Emulsification Activity ◽  
Carbon And Nitrogen ◽  
Emulsification Index

he effect of different cultural conditions on production of bioemulsifier from Serratia marcescens S10 was determined; different carbon and nitrogen sources were used such as: different oils include: edible (vegetable) oils (olive oil, sesame oil, sun flower oil and corn oil) and heavy oils (oil 150, oil 60, oil 40) as carbon sources and (NH4Cl, casein, (NH4)2SO4, peptone, tryptone, gelatin and yeast extract) as nitrogen sources were added to production media. Bioemulsifier was estimated by measuring the surface tension (S.T), emulsification activity (E.A) and emulsification index (E24%). The best results of bioemulsifier production from Serratia marcescens S10 were obtained at pH8 and incubated at 37ºC for 5days, using sesame oil as carbon source: surface tension (S.T) was reduced from 67 to 41 mN/m and with emulsification index (E24%) of 92% and emulsification activity (E.A) 0.3 and when used ammonium sulfate as nitrogen source: highest results for the isolate S10: S.T was decreased from 67 mN/m to 24 mN/m, E24% = 88%, E.A = 0.28.

Gordonia (nocardia) amarae foaming due to biosurfactant production

2002 ◽  
Vol 46 (1-2) ◽  
pp. 519-524 ◽  
Author(s):  
K.R. Pagilla ◽  
A. Sood ◽  
H. Kim
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Stationary Phase ◽  
Activated Sludge ◽  
Sole Carbon Source ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Biomass Concentration ◽  
Culture Broth ◽  
Biosurfactant Production

Gordonia amarae, a filamentous actinomycete, commonly found in foaming activated sludge wastewater treatment plants was investigated for its biosurfactant production capability. Soluble acetate and sparingly soluble hexadecane were used as carbon sources for G. amarae growth and biosurfactant production in laboratory scale batch reactors. The lowest surface tension (critical micelle concentration, CMC) of the cell-free culture broth was 55 dynes/cm when 1,900 mg/L acetate was used as the sole carbon source. The lowest surface tension was less than 40 dynes/cm when either 1% (v/v) hexadecane or a mixture of 1% (v/v) hexadecane and 0.5% (w/v) acetate was used as the carbon source. The maximum biomass concentration (the stationary phase) was achieved after 4 days when acetate was used along with hexadecane, whereas it took about 8 days to achieve the stationary phase with hexadecane alone. The maximum biosurfactant production was 3 × CMC with hexadecane as the sole carbon source, and it was 5 × CMC with the mixture of hexadecane and acetate. Longer term growth studies (∼ 35 days of culture growth) indicated that G. amarae produces biosurfactant in order to solubilize hexadecane, and that adding acetate improves its biosurfactant production by providing readily degradable substrate for initial biomass growth. This research confirms that the foaming problems in activated sludge containing G. amarae in the activated sludge are due to the biosurfactant production by G. amarae when hydrophobic substrates such as hexadecane are present.

scholarly journals Screening and Characterization of Biosurfactant-Producing Bacillus Species Isolated from Contaminated Soils in Makurdi Metropolis

2021 ◽  
Vol 37 (2) ◽  
pp. 165-176
Author(s):  
W.C John ◽  
I.O Ogbonna ◽  
G.M Gberikon ◽  
C.C Iheukwumere
Keyword(s):  
Surface Tension ◽  
Contaminated Soils ◽  
Biosurfactant Production ◽  
Bacillus Species ◽  
Screening Methods ◽  
Industrial Biotechnology ◽  
Emulsification Index ◽  
Bacillus Spp ◽  
Polymerase Chain

Biosurfactants synthesized by microorganisms are chemically diverse and have gained interest industrially due to their surface and interfacial tensions-reducing activities. In this study Bacillus species from contaminated soils were screened and characterized for biosurfactant production. The study was carried out at the Microbiology Laboratory, Federal University of Agriculture Makurdi, Nigeria. The Bacillus species were isolated from kerosene shops, palm oil shops, nearby restaurants, mechanic workshops and abattoir effluents- contaminated soil samples collected from Makurdi metropolis. The Bacillus spp. were screened for biosurfactants production potentials using various screening methods (oil spreading, beta haemolysis, drop collapse and emulsification index). Specific primers were used to amplify the srfAA (surfactin gene) gene in the Bacillus isolates and the nucleotide sequences were determined at Inqaba Biotec, South Africa. The screening results were statistically analysed using analysis of variance (ANOVA) at 95 % confidence level. Isolate RT7(4)B exhibited the ability to produce biosurfactant, as well as the highest emulsification index (E24) of 73.25 % while isolate PO7(3)C gave the highest oil displacement of 6.77 mm. The supernatant obtained from isolate RT7(4)B showed reduction in surface tension of up to 30.26 mN/m. The isolates gave positive results for biosurfactant production when subjected to drop collapse and Beta haemolytic tests. The Polymerase chain reaction (PCR) results revealed amplifications of srfAA gene from 7 isolates. Based on these findings, the isolates used in this study can be utilized for biosurfactant production, and can also be useful for bioremediation and industrial biotechnology applications. Keywords: Biosurfactants; emulsification index; Bacillus; surface tension; Drop collapse

scholarly journals Biosurfactant production by Pseudomonas aeruginosa MSIC02 in cashew apple juice using a 24 full factorial experimental design

2014 ◽  
Vol 20 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Maria Rocha ◽  
Jocélia Mendes ◽  
Maria Giro ◽  
Vânia Melo ◽  
Luciana Gonçalves
Keyword(s):  
Surface Tension ◽  
Experimental Design ◽  
Glucose Concentration ◽  
Apple Juice ◽  
Biosurfactant Production ◽  
Factorial Experimental Design ◽  
Cashew Apple Juice ◽  
Cashew Apple ◽  
Full Factorial Experimental Design ◽  
Full Factorial

In this work, the production of biosurfactants from cashew apple juice by P. aeruginosa MSIC02 was investigate by carrying out a 24 full factorial experimental design, using temperature, glucose concentration from cashew apple juice, phosphorous concentration and cultivation time as variables. The response variable was the percentage of reduction in surface tension in the cell-free culture medium, since it indicates the surface-active agent production. Maximum biosurfactant production, equivalent to a 58% reduction in surface tension, was obtained at 37?C, with glucose concentration of 5.0 g/L and no phosphorous supplementation. Surface tension reduction was significant, since low values were observed in the cell-free medium (27.50 dyne/cm), indicating that biosurfactant was produced. The biosurfactant emulsified different hydrophobic sources and showed stability in the face of salinity, exposure to high temperatures and extreme pH conditions. These physiochemical properties demonstrate the potential for using biosurfactants produced by P. aeruginosa MSIC02 in various applications.

scholarly journals Effect of Carbon Sources, Nitrogen Sources and Prebiotics on Growth of Saccharomyces Boulardii

2019 ◽  
Vol 23 (2) ◽  
pp. 101-108
Author(s):  
Xin Yang ◽  
Guowei Shu ◽  
Zhangteng Lei ◽  
Guanli Du ◽  
Zemin Liu ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Gastrointestinal Diseases ◽  
Potassium Nitrate ◽  
Soluble Starch ◽  
Saccharomyces Boulardii ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Dry Cell

Abstract Saccharomyces boulardii (S. boulardii) has been used as a probiotic for the prevention or treatment of various human gastrointestinal diseases for many years. Thus, S. boulardii has a wide range of application prospects in medicine and food industry. The experiments were investigated with effecting of carbon sources (galactose, sucrose, fructose, maltose, lactose, glucose, and soluble starch), nitrogen sources (tryptone, casein, yeast extract, peptone, soy peptone, beef extract and malted milk; ammonium sulfate, urea, diammonium hydrogen citrate, triammonium citrate, ammonium nitrate, ammonium chloride and potassium nitrate), prebiotics (xylo-oligosaccharide, isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, stachyose, raffinose and inulin) on the number of viable cells and dry cell weight of S. boulardii. The optimum concentration of 5 % glucose or sucrose, 2 % peptone or yeast extract, 0.4 % urea, 0.2 % fructo-oligosaccharide and 0.6 % galacto-oligosaccharide for S. boulardii grew better in medium.

scholarly journals Effect of Different Carbon Sources on Biosurfactants’ Production by Three Strains ofLactobacillusspp.

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Tene Hippolyte Mouafo ◽  
Augustin Mbawala ◽  
Robert Ndjouenkeu
Keyword(s):  
Surface Tension ◽  
Sugar Cane ◽  
Low Cost ◽  
High Surface ◽  
Carbon Sources ◽  
Antimicrobial Activities ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Cane Molasses ◽  
Emulsification Index

The potential of three indigenous bacterial strains (Lactobacillus delbrueckiiN2,Lactobacillus cellobiosusTM1, andLactobacillus plantarumG88) for the production of biosurfactants using sugar cane molasses or glycerol as substrates was investigated through emulsifying, surface tension, and antimicrobial activities. The different biosurfactants produced with molasses as substrate exhibited high surface tension reduction from 72 mN/m to values ranged from 47.50 ± 1.78 to 41.90 ± 0.79 mN/m and high emulsification index ranging from 49.89 ± 5.28 to 81.00 ± 1.14%. Whatever theLactobacillusstrain or the substrate used, the biosurfactants produced showed antimicrobial activities againstCandida albicansLV1, some pathogenic and/or spoilage Gram-positive and Gram-negative bacteria. The yields of biosurfactants with molasses (2.43 ± 0.09 to 3.03 ± 0.09 g/L) or glycerol (2.32 ± 0.19 to 2.82 ± 0.05 g/L) were significantly (p<0.05) high compared to those obtained with MRS broth as substrate (0.30 ± 0.02 to 0.51 ± 0.09 g/L). Preliminary characterization of crude biosurfactants reveals that they are mainly glycoproteins and glycolipids with molasses and glycerol as substrate, respectively. Therefore, sugar cane molasses or glycerol can effectively be used byLactobacillusstrains as low-cost substrates to increase their biosurfactants production.

scholarly journals Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile Microbacterium terrae KNR 9 Using Plackett-Burman Design

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Kiransinh N. Rajput ◽  
Kamlesh C. Patel ◽  
Ujjval B. Trivedi
Keyword(s):  
Yeast Extract ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Cyclodextrin Glucanotransferase ◽  
Corn Flour ◽  
Medium Components ◽  
Buffering Agent ◽  
Statistical Screening

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) production using new alkaliphile Microbacterium terrae KNR 9 was investigated by submerged fermentation. Statistical screening for components belonging to different categories, namely, soluble and raw starches as carbon sources, complex organic and inorganic nitrogen sources, minerals, a buffering agent, and a surfactant, has been carried out for CGTase production using Plackett-Burman factorial design. To screen out k (19), number of variables, k+1 (20), number of experiments, were performed. Among the fourteen components screened, four components, namely, soluble starch, corn flour, yeast extract, and K2HPO4, were identified as significant with reference to their concentration effect and corresponding p value. Although soluble starch showed highest significance, comparable significance was also observed with corn flour and hence it was selected as a sole carbon source along with yeast extract and K2HPO4 for further media optimization studies. Using screened components, CGTase production was increased to 45% and 87% at shake flask level and laboratory scale fermenter, respectively, as compared to basal media.

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