Distribution and population structure of Citrus tristeza virus in Poncirus trifoliata

2017 ◽  
Vol 46 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Yan Zhou ◽  
Yinjie Liu ◽  
Kehong Liu ◽  
Fangyun Yang ◽  
Changyong Zhou
2016 ◽  
Vol 162 (2) ◽  
pp. 409-423
Author(s):  
Cui Xiao ◽  
Run-Xian Yao ◽  
Fang Li ◽  
Su-Ming Dai ◽  
Grazia Licciardello ◽  
...  

2001 ◽  
Vol 75 (17) ◽  
pp. 8054-8062 ◽  
Author(s):  
Luis Rubio ◽  
Marı́a Angeles Ayllón ◽  
Ping Kong ◽  
Andres Fernández ◽  
MaryLou Polek ◽  
...  

ABSTRACT We examined the population structure and genetic variation of four genomic regions within and between 30 Citrus tristeza virus (CTV) isolates from Spain and California. Our analyses showed that most isolates contained a population of sequence variants, with one being predominant. Four isolates showed two major sequence variants in some genomic regions. The two major variants of three of these isolates showed very low nucleotide identity to each other but were very similar to those of other isolates, suggesting the possibility of mixed infections with two divergent isolates. Incongruencies of phylogenetic relationships in the different genomic regions and statistical analyses suggested that the genomes of some CTV sequence variants originated by recombination events between diverged sequence variants. No correlation was observed between geographic origin and nucleotide distance, and thus from a genetic view, the Spanish and Californian isolates analyzed here could be considered members of the same population.


Genetics ◽  
1998 ◽  
Vol 150 (2) ◽  
pp. 883-890
Author(s):  
D Q Fang ◽  
C T Federici ◽  
M L Roose

Abstract Resistance to citrus tristeza virus (CTV) was evaluated in 554 progeny of 10 populations derived from Poncirus trifoliata. A dominant gene (Ctv) controlled CTV resistance in P. trifoliata. Twenty-one dominant PCR-based DNA markers were identified as linked to Ctv by bulked segregant analysis. Of the 11 closest markers to Ctv, only 2 segregated in all populations. Ten of these markers were cloned and sequenced, and codominant RFLP markers were developed. Seven RFLP markers were then evaluated in 10 populations. Marker orders were consistent in all linkage maps based on data of single populations or on combined data of populations with similar segregation patterns. In a consensus map, the six closest marker loci spanned 5.3 cM of the Ctv region. Z16 cosegregated with Ctv. C19 and AD08 flanked Ctv at distances of 0.5 and 0.8 cM, respectively. These 3 markers were present as single copies in the Poncirus genome, and could be used directly for bacterial artificial chromosome library screening to initiate a walk toward Ctv. BLAST searches of the GenBank database revealed high sequence similarities between 2 markers and known plant disease resistance genes, indicating that a resistance gene cluster exists in the Ctv region in P. trifoliata.


1997 ◽  
Vol 95 (4) ◽  
pp. 691-695 ◽  
Author(s):  
P. F. Mestre ◽  
M. J. Asíns ◽  
E. A. Carbonell ◽  
L. Navarro

HortScience ◽  
1999 ◽  
Vol 34 (2) ◽  
pp. 334-335 ◽  
Author(s):  
D.Q. Fang ◽  
M.L. Roose

`Chandler' pummelo [Citrus maxima (Burm.) Merrill] was found to be citrus tristeza virus (CTV)–resistant. The inheritance of this resistance in 84 progeny of two crosses derived from `Chandler' pummelo and trifoliate orange [Poncirus trifoliata (L.) Raf.] was controlled by a single dominant gene designated Ctv2. Progeny analysis of four molecular markers closely linked to the Ctv gene, which confers resistance to CTV in trifoliate orange, demonstrated that Ctv2 was an independently assorting gene from Ctv.


Genome ◽  
2001 ◽  
Vol 44 (3) ◽  
pp. 382-393 ◽  
Author(s):  
Zhong-Nan Yang ◽  
Xin-Rong Ye ◽  
Sandong Choi ◽  
Joe Molina ◽  
Francis Moonan ◽  
...  

The citrus tristeza virus resistance gene (Ctv) is a single dominant gene in Poncirus trifoliata, a sexually compatible relative of citrus. To clone this gene, a bacterial artificial chromosome (BAC) library has been constructed from an individual plant that was homozygous for Ctv. This library contains 45 696 clones with an average insert size of 80 kb, corresponding to 9.6 genome equivalents. Screening of the BAC library with five chloroplast DNA probes indicated that 0.58% of the BAC clones contained chloroplast-derived inserts. The chromosome walk across the Ctv locus was initiated using three closely linked genetic markers: C19, AD8, and Z16. The walk has been completed and a contig of ca. 1.2 Mb was constructed. Based on new data, the genetic map in the Ctv region was revised, with Ctv being located between AD8-Z16 and C19 at distances of 1.2 and 0.6 cM, respectively. Utilizing DNA fragments isolated from the contig as RFLP markers, the Ctv locus was further mapped to a region of ca. 300 kb. This contig contains several putative disease-resistance genes similar to the rice Xa21 gene, the tomato Cf-2 gene, and the Arabidopsis thaliana RPS2 gene. This library will therefore allow cloning of Ctv and other putative disease-resistance genes.Key words: Poncirus, citrus tristeza virus, chromosome walk, resistance gene.


2009 ◽  
Vol 44 (7) ◽  
pp. 701-705 ◽  
Author(s):  
Jorgino Pompeu Junior ◽  
Silvia Blumer

O objetivo deste trabalho foi avaliar a produtividade e as características agronômicas de laranjeira 'Valência', enxertadas em porta-enxertos de híbridos de trifoliata (Poncirus trifoliata). A produção de frutos, a de sólidos solúveis totais por planta, as dimensões e a eficiência produtiva de copas de laranjeira 'Valência', enxertadas em 13 híbridos de trifoliata, cultivados sem irrigação, foram avaliados por períodos que variaram de três a oito anos. As plantas também foram avaliadas, visualmente, quanto à manifestação dos sintomas de tristeza (Citrus tristeza virus) e de declínio-dos-citros, e foi utilizado o teste diagnóstico "dot immunobinding assay" (DIBA), para detecção da ocorrência do declínio antes do aparecimento dos sintomas. As plantas tinham oito anos de idade, no início das avaliações. Verificou-se que o citrandarin 'Sunki' x 'English' induziu as maiores produções de frutos em oito colheitas, sem diferir significativamente do citrange 'Troyer'. Em três anos de análise, o citrandarin 'Sunki' x 'English', sem diferir dos citranges 'Troyer' e 'Carrizo', também induziu as maiores produções de frutos e sólidos solúveis por planta. O citrentin 'Clementina' x trifoliata, os citrandarins 'Cleópatra' x 'Swingle' (715) e (1.614), 'Cleópatra' x 'Rubidoux' (1.600) e 'Cleópatra' x 'Christian' induziram a formação de laranjeiras da cultivar Valência com alturas iguais ou inferiores a 2,5 m. Nenhuma das plantas apresentou sintomas de tristeza ou declínio-dos-citros. Foi constatada a incompatibilidade entre a cultivar Valência e o trangpur 'Cravo' x 'Carrizo'.


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