Large-Scale Production of Glaciozyma antarctica Antifreeze Protein 1 (Afp1) by Fed-Batch Fermentation of Pichia pastoris

2017 ◽  
Vol 43 (1) ◽  
pp. 133-141 ◽  
Author(s):  
Mahzan Md Tab ◽  
Noor Haza Fazlin Hashim ◽  
Nazalan Najimudin ◽  
Nor Muhammad Mahadi ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Batch Fermentation ◽  
Large Scale ◽  
Antifreeze Protein ◽  
Scale Production ◽  
Fed Batch ◽  
Large Scale Production ◽  
Fed Batch Fermentation

scholarly journals Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris — functional and kinetic asymmetry revealed in proteoliposomes

2013 ◽  
Vol 1828 (9) ◽  
pp. 2238-2246 ◽  
Author(s):  
Piero Pingitore ◽  
Lorena Pochini ◽  
Mariafrancesca Scalise ◽  
Michele Galluccio ◽  
Kristina Hedfalk ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production

scholarly journals High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris

2019 ◽  
Vol 21 (1) ◽  
pp. 279
Author(s):  
Qinghua Zhou ◽  
Zhixin Su ◽  
Liangcheng Jiao ◽  
Yao Wang ◽  
Kaixin Yang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Yarrowia Lipolytica ◽  
Large Scale ◽  
Gene Dosage ◽  
Life Time ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Scale Production ◽  
Large Scale Production ◽  
High Level

As a promising biocatalyst, Yarrowia lipolytica lipase 2 (YlLip2) is limited in its industrial applications due to its low thermostability. In this study, a thermostable YlLip2 mutant was overexpressed in Pichia pastoris and its half-life time was over 30 min at 80 °C. To obtain a higher protein secretion level, the gene dosage of the mutated lip2 gene was optimized and the lipase activity was improved by about 89%. Then, the YlLip2 activity of the obtained strain further increased from 482 to 1465 U/mL via optimizing the shaking flask culture conditions. Subsequently, Hac1p and Vitreoscilla hemoglobin (VHb) were coexpressed with the YlLip2 mutant to reduce the endoplasmic reticulum stress and enhance the oxygen uptake efficiency in the recombinant strains, respectively. Furthermore, high-density fermentations were performed in a 3 L bioreactor and the production of the YlLip2 mutant reached 9080 U/mL. The results demonstrated that the expression level of the thermostable YlLip2 mutant was predominantly enhanced via the combination of these strategies in P. pastoris, which forms a consolidated basis for its large-scale production and future industrial applications.

Maximization of Production of Secreted Recombinant Proteins in Pichia pastoris Fed-Batch Fermentation

2008 ◽  
Vol 21 (2) ◽  
pp. 386-393 ◽  
Author(s):  
Wenhui Zhang ◽  
Jayanta Sinha ◽  
Leonard A. Smith ◽  
Mehmet Inan ◽  
Michael M. Meagher
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Proteins ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation

Cloning, large-scale production and characterization of fusion protein (P-TUFT-ALT-2) of Brugian abundant larval transcript-2 with tuftsin in Pichia pastoris

2018 ◽  
Vol 48 (9) ◽  
pp. 823-833 ◽  
Author(s):  
Rajkumar Paul ◽  
Selvarajan Karthik ◽  
Ponnusamy Vimalraj ◽  
Sankaranarayanan Meenakshisundaram ◽  
Perumal Kaliraj
Keyword(s):  
Pichia Pastoris ◽  
Fusion Protein ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production
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