Species delimitation and sex associations in the bee genus Thygater, with the aid of molecular data, and the description of a new species

Abstract Neoechinorhynchus is one of the most speciose genera of acanthocephalans, with approximately 116 described species. A recent study, aimed at establishing the genetic diversity of Neoechinorhynchus in Middle American freshwater fishes, validated nine species molecularly and morphologically and revealed the existence of 10 putative candidate species. Neoechinorhynchus golvani, a parasite commonly found in cichlids throughout Middle America with an allegedly large intraspecific morphological variability, was found to represent a species complex; species delimitation methods uncovered three additional genetic lineages. Here, we re-analyse the morphological and molecular data for N. golvani species complex infecting cichlids in that geographical area. A multivariate analysis of variance (MANOVA) was conducted particularly for the length of apical, middle and posterior hooks of the species/lineages of Neoechinorhynchus in cichlids, revealing morphological variation in the length of apical hooks for Lineage 8, although no morphological distinction was observed for Lineages 9 and 10. A new concatenated phylogenetic analysis of one mitochondrial and two ribosomal DNA genes was used to further corroborate the species delimitation among lineages; Neoechinorhynchus Lineage 8 was found to be morphologically and genetically distinct from its sister taxa, N. golvani and other two undescribed genetic lineages, and is formally described as a new species. Neoechinorhynchus costarricense n. sp. is described from the intestines of eight species of cichlids in Costa Rica. The new species is distinguished from the other species/lineages of Neoechinorhynchus in cichlids mainly by the size of the apical hooks of the proboscis.

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Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City

ZooKeys ◽

10.3897/zookeys.892.39558 ◽

2019 ◽

Vol 892 ◽

pp. 93-133 ◽

Cited By ~ 4

Author(s):

Alejandro Valdez-Mondragón ◽

Claudia I. Navarro-Rodríguez ◽

Karen P. Solís-Catalán ◽

Mayra R. Cortez-Roldán ◽

Alma R. Juárez-Sánchez

Keyword(s):

New Species ◽

Mexico City ◽

Molecular Species ◽

Species Delimitation ◽

Morphological Characters ◽

Molecular Data ◽

Integrative Taxonomy ◽

Taxonomic Approach ◽

Unique Species ◽

A New Species

A new species of the spider genus Loxosceles Heineken & Lowe, 1832, Loxosceles tenochtitlan Valdez-Mondragón & Navarro-Rodríguez, sp. nov., is described based on adult male and female specimens from the states of Mexico City, Estado de Mexico and Tlaxcala. Integrative taxonomy including traditional morphology, geometric and lineal morphology, and molecules (DNA barcodes of cytochrome c oxidase subunit 1 (CO1) and internal transcribed spacer 2 (ITS2)), were used as evidence to delimit the new species. Four methods were used for molecular analyses and species delimitation: 1) corrected p-distances under neighbor joining (NJ), 2) automatic barcode gap discovery (ABGD), 3) general mixed yule coalescent model (GMYC), and 4) poisson tree processes (bPTP). All molecular methods, traditional, geometric and lineal morphology were consistent in delimiting and recognizing the new species. Loxosceles tenochtitlansp. nov. is closely related to L. misteca based on molecular data. Although both species are morphologically similar, the average p-distance from CO1 data was 13.8% and 4.2% for ITS2 data. The molecular species delimitation methods recovered well-supported monophyletic clusters for samples of L. tenochtitlansp. nov. from Mexico City + Tlaxcala and for samples of L. misteca from Guerrero. Loxosceles tenochtitlansp. nov. is considered a unique species for three reasons: (1) it can be distinguished by morphological characters (genitalic and somatic); (2) the four different molecular species delimitation methods were congruent to separate both species; and (3) there is variation in leg I length of males between both species, with the males of L. misteca having longer legs than males of L. tenochtitlansp. nov., also morphometrically, the shape of tibiae of the palp between males of both species is different.

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A New Species of the Genus Thermophis(Serpentes: Colubridae) from Shangri-La, Northern Yunnan, China, with a Proposal for an Eclectic Rule for Species Delimitation

Asian Herpetological Research ◽

10.3724/sp.j.1245.2014.00228 ◽

2014 ◽

Vol 5 (4) ◽

pp. 228 ◽

Cited By ~ 5

Author(s):

PENG Lifang ◽

LU Changhu ◽

HUANG Song ◽

GUO Peng ◽

ZHANG Yaping

Keyword(s):

New Species ◽

Species Delimitation ◽

A New Species

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Description of a new species Coomansus batxatensis (Mononchida, Mononchidae) from Vietnam, with an updated key to species

Journal of Helminthology ◽

10.1017/s0022149x21000201 ◽

2021 ◽

Vol 95 ◽

Author(s):

T.T.T. Vu

Keyword(s):

New Species ◽

Buccal Cavity ◽

Small Body ◽

Molecular Data ◽

Cavity Size ◽

Key To Species ◽

Tooth Apex ◽

A New Species ◽

Posterior Position ◽

28S Ribosomal Dna

Abstract Coomansus batxatensis sp. nov., recorded from Vietnam, is described and illustrated and its phylogenetic relationship within the Mononchida is analysed. The molecular data (18S and 28S ribosomal DNA) are provided for the new species. The new species is characterized by small body size (body length, L = 0.7–0.9 mm); buccal cavity sub-rectangular in shape, flattened at base, 21–24 × 12–13 μm or 1.9 (1.7–2.0) times as long as wide; posterior position of dorsal tooth apex (59–63% from the base of buccal cavity); pars refringens vaginae with faint and small (2.5 × 1.7 μm) teardrop-shaped pieces, short pars distalis vaginae; and males with short spicules (50–51.5 μm) with rounded head and conical blade part. The new species is close to Coomansus parvus but differs from it by the smaller buccal cavity size, more posterior position of the dorsal tooth apex, longer tail and presence of males. An updated identification key to Coomansus species and a compendium of all the species known are presented.

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A new species of Cynomops (Chiroptera: Molossidae) from the northwestern slope of the Andes

Mammalia ◽

10.1515/mammalia-2020-0068 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Daniela Arenas-Viveros ◽

Pamela Sánchez-Vendizú ◽

Alan Giraldo ◽

Jorge Salazar-Bravo

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Molecular Data ◽

Mtdna Sequences ◽

The Andes ◽

Western Side ◽

Morphological And Molecular Data ◽

A New Species ◽

Lines Of Evidence

Abstract The systematics and taxonomy of the broadly distributed bats of the genus Cynomops has changed considerably in the last few years. Among the major changes, Cynomops abrasus was split into two species of large-bodied forms (Cynomops mastivus and C. abrasus) distributed east of the Andes. However, large Colombian specimens identified as C. abrasus from the western side of the Andes had yet to be included in any revisionary work. Phylogenetic analysis performed in this study, using mtDNA sequences (Cytochrome-b), revealed that these Colombian individuals are more closely related to Cynomops greenhalli. Morphological and molecular data allowed us to recognize populations from western Colombia, western Ecuador and northwestern Peru, as members of a new species of Cynomops. Characters that allow for its differentiation from C. greenhalli include a larger forearm, paler but more uniform ventral pelage, more globular braincase, and well-developed zygomatic processes of the maxilla (almost reaching the postorbital constriction). This study serves as another example of the importance of including multiple lines of evidence in the recognition of a new species. Given its rarity and the advanced transformation of its habitat, this new species is particularly important from a conservation perspective.

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Taxonomic assessment of the genus Procamallanus (Nematoda) in Middle American cichlids (Osteichthyes) with molecular data, and the description of a new species from Nicaragua and Costa Rica

Parasitology Research ◽

10.1007/s00436-021-07148-1 ◽

2021 ◽

Author(s):

Ana Santacruz ◽

Marta Barluenga ◽

Gerardo Pérez-Ponce de León

Keyword(s):

New Species ◽

Costa Rica ◽

Molecular Data ◽

A New Species

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Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

Journal of Helminthology ◽

10.1017/s0022149x21000109 ◽

2021 ◽

Vol 95 ◽

Author(s):

M.M. Montes ◽

J. Barneche ◽

Y. Croci ◽

D. Balcazar ◽

A. Almirón ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

National Park ◽

Molecular Data ◽

Rrna Gene ◽

28S Rrna ◽

The Family ◽

Parasitological Survey ◽

Morphological And Molecular Data ◽

A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

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